Aldosterone effects on electrolyte transport of the lower intestine (coprodeum and colon) of the fowl (Gallus domesticus) in vitro

Thomas, David Hurst; Jallageas, Monique; Munck, B. G.; Skadhauge, Erik

doi:10.1016/0016-6480(80)90094-5

Cited by 54 publications

(19 citation statements)

References 11 publications

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“…With our dietary VT: regime we obtained a twofold higher PA level. Commercial diet in the study from Thomas et al (1980) decreased PA levels to 160 • 37 pM. The commercial diet in their study is comparable with our HS diet, but the concentration of circulating aldosterone was also under the limit of detection in hens with HS diet in our observations.…”

Section: Plasma Aldosterone Levelssupporting

confidence: 86%

“…Thomas et al (1980) showed similar effects. With wheat and barley diet (LS) the PA level in the study from Thomas et al (1980) reached 265 _+ 17 pM. With our dietary VT: regime we obtained a twofold higher PA level.…”

Section: Plasma Aldosterone Levelssupporting

confidence: 76%

“…As discussed previously (Thomas et al, 1980;Skadhauge et al, 1983;Arnason et al, 1986), the concentration of plasma aldosterone (PA) is high in low-NaC1 fed birds and decreased by resalination. The spironolactone dose in our study lowered PA level in one group of LS hens to about half the control values; in the second group with the higher spironolactone dosage, the PA level was below the limit of detection.…”

Section: Endogenous Aldosterone Levelmentioning

confidence: 88%

“…Because coprodeal epithelia under HS conditions exhibit no Na current and are poorly amiloride-sensitive (Thomas et al, 1980) it was not possible to apply our method to this dietary condition and to obtain permeability measurements for low Na-transporting coprodeal epithelia (Bindslev, 1979).…”

Section: Depolarizationmentioning

confidence: 98%

“…Aldosterone induces new transport sites (Bindslev, 1979;Eldrup, Mollgard & Bindslev, 1980) and they seem to originate from preformed cytoplasmic vesicles below the apical cell surface . Recent investigators compared the different time-course of aldosterone injections (Thomas et al, 1980;Clauss et al, 1984) with the time-course of NaC1 depletion or restoration (Thomas & Skadhauge, 1979Skadhauge et al, 1983;Clauss et al, 1984). All these studies indicated a time-dependent dietary stimulation of Na transport, and that in spite of already augmented plasma aldosterone levels after two days (Arnason et al, 1986) 'it takes several days for the epithelium to achieve its maximal transport rate.…”

Section: Introductionmentioning

confidence: 94%

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Effects of adrenal steroids on Na transport in the lower intestine (Coprodeum) of the hen

et al. 1987

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The influence of adrenal steroids on sodium transport in hen coprodeum was investigated by electrophysiological methods. Laying hens were maintained on low-NaCl diet (LS), or on high-NaCl diet (HS). HS hens were pretreated with aldosterone (128 micrograms/kg) or dexamethasone (1 mg/kg) before experiment. A group of LS hens received spironolactone (70 or 160 mg/kg, for three days). The effects of these dietary and hormonal manipulations on the amiloride-sensitive part of the short-circuit current were examined. This part is in excellent agreement with the net Na flux, and therefore a direct electrical measurement for Na transport. After depolarizing the basolateral membrane potential with a high K concentration, the apical Na permeability and the intracellular Na activity were investigated by current-voltage relations for the different experimental conditions. Plasma aldosterone concentrations (PA) were low in HS hens, dexamethasone-treated HS hens and spironolactone-treated LS hens (less than 70 pM). In contrast LS hens and aldosterone-treated HS hens had a PA concentration of 596 +/- 70 and 583 +/- 172 pM, respectively. LS diet (chronic stimulation) had the largest stimulatory effect on Na transport and apical Na permeability. Hormone-treated animals had three- to fourfold lower values. Spironolactone supply in LS hens decreased Na transport and apical Na permeability about 50%. The results provide evidence that both mineralo- and gluco-corticoids stimulate Na transport in this tissue by increasing the apical Na permeability. Quantitative differences between acute and chronic stimulation reveal a secondary slower adaptation in apical membrane properties.

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Section: Plasma Aldosterone Levelssupporting

confidence: 86%

Section: Plasma Aldosterone Levelssupporting

confidence: 76%

Section: Endogenous Aldosterone Levelmentioning

confidence: 88%

Section: Depolarizationmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 94%

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Effects of adrenal steroids on Na transport in the lower intestine (Coprodeum) of the hen

et al. 1987

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Physiological roles and regulation of transport activities in the avian lower intestine

Laverty

Skadhauge

1999

J. Exp. Zool.

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Urates and allantoic regulation in embryonic Japanese quail, Coturnix coturnix japonica

McNabb

1986

J. Exp. Zool.

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During embryonic development, allantoic fluid represents the shifting balance between renal excretion and reabsorption by chorioallantoic membranes. Allantoic contents of Na+, K+, Cl-, urate, pH, and water were followed over days 10-15 of the 16 day incubation. Water volume remained near 0.9 ml until day 13, then declined very rapidly. The pH declined more steadily, from 8 to 5.5. Contents of Na+ and Cl- fell regularly to final values 80-88% below day 10. The K+ content changed differently and nearly doubled by day 13 but returned to day 10 values at the end. Urate content rose until day 13, then fell suddenly to low levels. This was due to the abrupt precipitation of most urate into masses not sampled by our method, so that after day 13, urate was underestimated (probably by 90-96%). Ion binding by urates was low (about 3% of Na+ and Cl-, 10% of K+) and appeared to be nonspecific. The underestimate of urate contents means, however, that in late incubation about one third of allantoic Na+ and 65-70% of K+ and Cl- are bound to precipitated urate and do not appear in balance sheets of allantoic ions. These precipitated ions account for the significant amounts of Na+ and K+ that remain in the allantoic remnant, left in the eggshell after hatching, but whose presence is not predicted by analysis of allantoic fluid.

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Aldosterone effects on electrolyte transport of the lower intestine (coprodeum and colon) of the fowl (Gallus domesticus) in vitro

Cited by 54 publications

References 11 publications

Effects of adrenal steroids on Na transport in the lower intestine (Coprodeum) of the hen

Effects of adrenal steroids on Na transport in the lower intestine (Coprodeum) of the hen

Physiological roles and regulation of transport activities in the avian lower intestine

Urates and allantoic regulation in embryonic Japanese quail, Coturnix coturnix japonica

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