Alexandrium pacificum Litaker sp. nov (Group IV): Resting cyst distribution and toxin profile of vegetative cells in Bizerte Lagoon (Tunisia, Southern Mediterranean Sea)

Fertouna-Bellakhal, Mouna; Dhib, Amel; Fathalli, Afef; Bellakhal, Meher; Chomérat, Nicolas; Masseret, Estelle; Laabir, Mohamed; Turki, Souâd; Aleya, Lotfi

doi:10.1016/j.hal.2015.07.007

Cited by 43 publications

(15 citation statements)

References 70 publications

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“…catenella , [ 56 ]) was cultured under the same conditions but at a temperature of 20°C, which corresponds to its optimal growth [ 57 ]. OOBZT14, PLBZT14, and CMBZT14 strains were isolated from the Bizerte Bay [ 34 ] while the ABZ1 strain was obtained from the culture collection of the Center for Marine Biodiversity, Exploitation and Conservation (Montpellier University, France) and was originally isolated from the Bizerte lagoon [ 58 ]. Both sites are located in Northern Tunisia, Southern Mediterranean Sea.…”

Section: Methodsmentioning

confidence: 99%

New insights on the species-specific allelopathic interactions between macrophytes and marine HAB dinoflagellates

et al. 2017

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Macrophytes are known to release allelochemicals that have the ability to inhibit the proliferation of their competitors. Here, we investigated the effects of the fresh leaves of two magnoliophytes (Zostera noltei and Cymodocea nodosa) and thalli of the macroalgae Ulva rigida on three HAB-forming benthic dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima, and Coolia monotis). The effects of C. nodosa and U. rigida were also tested against the neurotoxic planktonic dinoflagellate Alexandrium pacificum Litaker sp. nov (former Alexandrium catenella). Co-culture experiments were conducted under controlled laboratory conditions and potential allelopathic effects of the macrophytes on the growth, photosynthesis and toxin production of the targeted dinoflagellates were evaluated. Results showed that U. rigida had the strongest algicidal effect and that the planktonic A. pacificum was the most vulnerable species. Benthic dinoflagellates seemed more tolerant to potential allelochemicals produced by macrophytes. Depending on the dinoflagellate/macrophyte pairs and the weight of leaves/thalli tested, the studied physiological processes were moderately to heavily altered. Our results suggest that the allelopathic activity of the macrophytes could influence the development of HAB species.

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Section: Methodsmentioning

confidence: 99%

New insights on the species-specific allelopathic interactions between macrophytes and marine HAB dinoflagellates

et al. 2017

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“…The planktonic dinoflagellate Alexandrium catenella (ABZ1) was cultured under the same conditions, but at a temperature of 20 °C and salinity 35 corresponding to its optimal growth. OOBZT14 and ABZ1 strains were isolated in the Bay and the lagoon of Bizerte (Western Mediterranean Tunisia), respectively (Ben-Gharbia et al, 2016;Fertouna-Bellakhal et al, 2015 Cu and 0.085,0.27,0.85,2.7,8.5,27 and 85 nM of TBT.…”

Section: Dinoflagellate Culturesmentioning

confidence: 99%

“…In the context of the increasing anthropization of marine environments, particularly in confined waters (e.g. harbours or lagoons), where A. catenella forms recurrent blooms (Fertouna-Bellakhal et al, 2015;Laanaia et al, 2013), this dinoflagellate could potentially take advantage of this higher tolerance to contaminants in impacted marine ecosystems.…”

Section: Microalgae Growthmentioning

confidence: 99%

Effects of copper and butyltin compounds on the growth, photosynthetic activity and toxin production of two HAB dinoflagellates: The planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata

et al. 2018

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Controlled laboratory experiments were conducted to test the effects of copper (Cu) and butyltins (BuT) on the growth, photosynthetic activity and toxin content of two HABs (Harmful Algal Blooms) dinoflagellates, the planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata. Microalgae were exposed to increasing concentrations of Cu (10 to 31 nM) or BuT (0.084 to 84 nM) for seven days. When considering the growth, EC values were 0.16 (±0.09) nM and 0.03 (±0.02) nM of Cu for A. catenella and O. cf. ovata, respectively. Regarding BuT, EC was 14.2 (±6) nM for O. cf. ovata, while A. catenella growth inhibition appeared at BuT concentrations ≥27 nM. Photosynthetic activity of the studied dinoflagellates decreased with increasing Cu and BuT concentrations. For O. cf. ovata, the response of this physiological parameter to contamination was less sensitive than the biomass. Cu exposure induced the formation of temporary cysts in both organisms that could resist adverse conditions. The ovatoxin-a and -b concentrations in O. cf. ovata cells increased significantly in the presence of Cu. Altogether, the results suggest a better tolerance of the planktonic A. catenella to Cu and BuT. This could result in a differentiated selection pressure exerted by these metals on phytoplankton species in highly polluted waters. The over-production of toxins in response to Cu stress could pose supplementary health and socio-economic threats in the contaminated marine ecosystems where HABs develop.

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“…For aquaculture activities, mass mortalities lead to production losses with severe economic impacts (Hoagland et al, 2002;Park et al, 2013). Among HAB dinoflagellates, the invasive Alexandrium catenella was originally identified in the Gulf of California (Sommer et al, 1937), but its occurrence is now reported in many marine ecosystems in France, Japan, Korea, South Africa and Tunisia (Vila et al, 2001;Masseret et al, 2009;Anderson et al, 2012;Laabir et al, 2012;Fertouna-Bellakhal et al, 2015). Alexandrium catenella produces a saxitoxin and its derivatives, which are responsible for the paralytic shellfish poisoning (PSP) syndrome (Anderson, 1994;Anderson et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

In silicoprediction of the secretome from the invasive neurotoxic marine dinoflagellateAlexandrium catenella

Chetouhi

Laabir

Masseret

et al. 2019

Environ Microbiol Rep

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Summary Alexandrium catenella, a marine dinoflagellate responsible for harmful algal blooms (HABs), proliferates with greater frequency, distribution and intensity, in disturbed marine coastal ecosystems. The proteins secreted into seawater may play a crucial role in maintaining this dinoflagellate in these ecosystems, but this possibility has never been investigated before. In this study, the A. catenella secretome was predicted from its transcriptome by combining several bioinformatics tools. Our results predict a secretome of 2 779 proteins, among which 79% contain less than 500 amino acids, suggesting that most secreted proteins are short in length. The predicted secretome includes 963 proteins (35%) with Pfam domains: 773 proteins with one Pfam domain and 190 proteins with two or more Pfam domains. Their functional annotation showed that they are mainly involved in (i) proteolysis, (ii) stress responses and (iii) primary metabolism. In addition, 47% of the secreted proteins appear to be enzymes, primarily peptidases, known to be biologically active in the extracellular medium during stress responses. Finally, this study provides a wealth of candidates of proteins secreted by A. catenella, which may interact with the marine environment and help this dinoflagellate develop in various environmental conditions.

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Alexandrium pacificum Litaker sp. nov (Group IV): Resting cyst distribution and toxin profile of vegetative cells in Bizerte Lagoon (Tunisia, Southern Mediterranean Sea)

Cited by 43 publications

References 70 publications

New insights on the species-specific allelopathic interactions between macrophytes and marine HAB dinoflagellates

New insights on the species-specific allelopathic interactions between macrophytes and marine HAB dinoflagellates

Effects of copper and butyltin compounds on the growth, photosynthetic activity and toxin production of two HAB dinoflagellates: The planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata

In silicoprediction of the secretome from the invasive neurotoxic marine dinoflagellateAlexandrium catenella

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