Alfalfa as a vegetable source of β-carotene: The change mechanism of β-carotene during fermentation

Zong, Cheng; Shao, Tao; Chiou, Jiachi; Wu, Aili; Huang, Zhongyong; Chen, Chen; Zhu, Jiugang; Zhihao, Dong; Liu, Qinhua; Li, Mao

doi:10.1016/j.foodres.2023.113104

Cited by 4 publications

(1 citation statement)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The α-tocopherol and carotenoids concentrations in diets could influence vitamin content in milk, as well as induce positive effects on antioxidant activity and the immune status of animals [ 33 – 35 ]. The higher concentrations of α-tocopherol and β-carotene observed in the L. plantarum 24-7 treated silage might be due to the strain’s ability to prevent α-tocopherol and β-carotene from being degraded or to promote biosynthesis of these two substances by microbes in silage [ 36 , 37 ], which could modulate the OS status. In addition, the changes in T-AOC, GSH-Px, CAT as well as SOD activities of the baled silages inoculated with the antioxidant LAB strain were similar to those observed for silage ensiled in the laboratory silos, which mainly exhibited an increased T-AOC, GSH-Px, and CAT with a decreased SOD [ 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of antioxidant-rich Lactiplantibacillus plantarum inoculated alfalfa silage on rumen fermentation, antioxidant and immunity status, and mammary gland gene expression in dairy goats

Zhang,

Usman,

et al. 2024

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background Milk synthesis in lactating animals demands high energy metabolism, which results in an increased production of reactive oxygen metabolites (ROM) causing an imbalance between oxidants and antioxidants thereby inducing oxidative stress (OS) on the animals. To mitigate OS and postpartum disorders in dairy goats and gain insight into the impact of dietary choices on redox status during lactation, a feeding trial was conducted using alfalfa silage inoculated with a high-antioxidant strain of Lactiplantibacillus plantarum. Methods Twenty-four Guanzhong dairy goats (38.1 ± 1.20 kg) were randomly assigned to two dietary treatments: one containing silage inoculated with L. plantarum MTD/1 (RSMTD-1), and the other containing silage inoculated with high antioxidant activity L. plantarum 24-7 (ES24-7). Results ES24-7-inoculated silage exhibited better fermentation quality and antioxidant activity compared to RSMTD-1. The ES24-7 diet elevated the total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities in milk, serum, and feces of lactating goats (with the exception of T-AOC in milk). Additionally, the diet containing ES24-7 inoculated silage enhanced casein yield, milk free fatty acid (FFA) content, and vitamin A level in the goats’ milk. Furthermore, an increase of immunoglobulin (Ig)A, IgG, IgM, interleukin (IL)-4, and IL-10 concentrations were observed, coupled with a reduction in IL-1β, IL-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α concentrations in the serum of lactating goats fed ES24-7. Higher concentrations of total volatile fatty acid (VFA), acetate, and propionate were observed in the rumen fluid of dairy goats fed ES24-7 inoculated silage. Moreover, the diet containing ES24-7 inoculated silage significantly upregulated the expression of nuclear factor erythroid 2 like 2 (NFE2L2), beta-carotene oxygenase 1 (BCO1), SOD1, SOD2, SOD3, GPX2, CAT, glutathione-disulfide reductase (GSR), and heme oxygenase 1 (HMOX1) genes in the mammary gland, while decreased the levels of NADPH oxidase 4 (NOX4), TNF, and interferon gamma (IFNG). Conclusions These findings indicated that feeding L. plantarum 24-7 inoculated alfalfa silage not only improved rumen fermentation and milk quality in lactating dairy goats but also boosted their immunity and antioxidant status by modulating the expression of several genes related to antioxidant and inflammation in the mammary gland.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of antioxidant-rich Lactiplantibacillus plantarum inoculated alfalfa silage on rumen fermentation, antioxidant and immunity status, and mammary gland gene expression in dairy goats

Zhang,

Usman,

et al. 2024

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

High internal phase emulsions stabilized by fluorescent phycocyanin for improved stability and bioaccessibility of β‐carotene

Cheng,

Sun,

Zhang

et al. 2024

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUNDHigh internal phase emulsions (HIPE) are distinguished from ordinary emulsions by higher oil‐phase percentage and better storage stability. Recently, HIPE stabilized with protein‐based particles has received more attention. However, organic precipitation, chemical cross‐linking and thermal denaturation are often needed to stabilize emulsions with natural proteins, and there is an urgent need to reduce the pollution of organic reagents.RESULTSHIPE loaded with β‐carotene stabilized by phycocyanin was prepared under mild conditions. It demonstrated strong stability in terms of temperature and storage, as evidenced by its 94.17% retention rate and 81.06% bioavailability. This stability was ascribed to the efficient defense against heat and UV rays, which was probably associated with the oil‐droplet environment and interfacial protection of phycocyanin. It is speculated that the possible main interaction site between phycocyanin and sorbitol exists near amino acids 110 to 120 of the B chain. The hydrogen bond and hydrophobic interaction between them make the phycocyanin fully adsorbed on the oil–water interface when sorbitol is stable, forming a strong oil–water structure, which increases the stability of the emulsion.CONCLUSIONThe outstanding fluorescence characteristics provide a feasible alternative for fluorescent emulsions to distribute and trace active compounds in vitro. HIPE loaded with β‐carotene might have potential as a 3D printing material for edible functional foods. © 2024 Society of Chemical Industry.

show abstract

Hexanoic acid addition helps to clarify the possible mechanisms of the increased β-carotene content during alfalfa fermentation

ZONG,

ZHAO,

JIANG

et al. 2024

Journal of Integrative Agriculture

View full text Add to dashboard Cite

Alfalfa as a vegetable source of β-carotene: The change mechanism of β-carotene during fermentation

Cited by 4 publications

References 41 publications

Effects of antioxidant-rich Lactiplantibacillus plantarum inoculated alfalfa silage on rumen fermentation, antioxidant and immunity status, and mammary gland gene expression in dairy goats

Effects of antioxidant-rich Lactiplantibacillus plantarum inoculated alfalfa silage on rumen fermentation, antioxidant and immunity status, and mammary gland gene expression in dairy goats

High internal phase emulsions stabilized by fluorescent phycocyanin for improved stability and bioaccessibility of β‐carotene

Hexanoic acid addition helps to clarify the possible mechanisms of the increased β-carotene content during alfalfa fermentation

Contact Info

Product

Resources

About