This study examines the numerous pre-processing techniques used to convert biomass into greater sustainable biofuels and commodities, highlighting the boom in productivity and the supply of a greater uniform, dry, and suitable feedstock. by using addressing the demanding situations associated with biomass size, layout, moisture content, and variability, this study delves into mechanical procedures, drying, torrefaction, palletization, hydrolysis, hydrothermal, and microwave-primarily based strategies as possible solutions. It explores the utilization of various biomass types, which include wood, woody biomass, herbaceous streams, and agricultural streams, and assesses their impact on bioenergy production and environmental sustainability. The study also considers the position of algae, specially microalgae, in offering bioactive materials with substantial health advantages and their ability in overcoming land-use concerns associated with traditional biomass. moreover, the paper evaluates the environmental affects and sustainability of biomass usage, advocating for microalgae as a promising feedstock for 1/3-technology biofuels. The research is grounded in the context of the increasing need to reduce reliance on fossil fuels due to urbanization and population enlargement, which make contributions to environmental degradation.