Algal photosynthesis converts nitric oxide into nitrous oxide

Abstract

One sentence summary: Green microalgae produce N2O using flavodiiron proteins in the light and a cytochrome P450 NO reductase in the dark. AbstractNitrous oxide (N2O), a potent greenhouse gas in the atmosphere, is produced mostly from aquatic ecosystems, to which algae substantially contribute. However, mechanisms of N2O production by photosynthetic organisms are poorly described. Here, we show that the green microalga Chlamydomonas reinhardtii reduces NO into N2O using the photosynthetic electron transport. Through the study of C. reinhardtii mutants deficient in flavodiiron proteins (FLVs) or in a cytochrome p450 (CYP55), we show that FLVs contribute to NO reduction in the light, while CYP55 operates in the dark. Furthermore, NO reduction by both pathways is restricted to Chlorophytes, organisms particularly abundant in ocean N2O-producing hotspots. Our results provide a mechanistic understanding of N2O production in eukaryotic phototrophs and represent an important step toward a comprehensive assessment of greenhouse gas emission by aquatic ecosystems.