Algerian Propolis Potentiates Doxorubicin Mediated Anticancer Effect Against Human Pancreatic PANC-1 Cancer Cell Line through Cell Cycle Arrest, Apoptosis Induction and P-Glycoprotein Inhibition

Rouibah, Hassiba; Kebsa, Wided; Lahouel, Mesbah; Zihlif, Malek; Ahram, Mamoun; Aburmeleih, Bachaer; Mustafa, Ebtihal; Al‐Ameer, Hamzeh J.

doi:10.2174/1871520618666180110143239

Cited by 16 publications

(6 citation statements)

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“…Hence, treating the prostate cancer cells is quite hard and thus a new therapeutic strategy should be developed by focusing more on anti-inflammatory and antioxidative drugs. Existing evidence has conferred that propolis and its active components can strongly suppress the proliferation and migration as well as enhance apoptosis in various prostate cancer cell lines like LNCaP, PC-3, PANC-1, and DU-145 [77,79].…”

Section: Chemopreventive On Prostate Cancer and Underlying Mechanismsmentioning

confidence: 99%

“…Propolis and its components display strong anti-proliferative activity via suppression of proliferating cell nuclear antigen and vascular cell adhesion molecule 1 in human prostate PC-3 cancer cells [80]. Also, propolis supplementation could significantly arrest the cell cycle in prostate cancer cells by modulation of the expression of cyclin A, B, and D1, and Cdk as well as p21 [79,81]. Moreover, propolis can induce apoptosis by decreasing the cellular levels of apoptosis inhibitor proteins including cellular inhibitor apoptosis protein 1/2 (cIAP-1/2) [81,82].…”

Section: Chemopreventive On Prostate Cancer and Underlying Mechanismsmentioning

confidence: 99%

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Chemopreventive and Chemotherapeutic Effect of Propolis and Its Constituents: A Mini-review

et al. 2020

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Propolis is a bee wax rich in various phytocomponents and traditionally used to treat various ailments. Propolis is reported to possess an array of biological properties including anti-inflammatory, antioxidant, anti-cancer, and anti-diabetic as well as cardioprotective, hepatoprotective, renoprotective, and derma protective activities. A plethora of studies confirmed that propolis is effective against various types of cancer including head and neck, lung, liver, brain (glioma), pancreas, kidney, prostate, skin (melanoma), breast, oral, esophagus, gastric, colorectal, and bladder cancers. However, many researchers have demonstrated that propolis displays potent chemoprotective/chemopreventive or anti-cancer activity against only a few types of cancers like oral, gastrointestinal, dermal (melanoma), breast, and prostate cancers. Therefore, this mini-review only summarizes the chemopreventive/chemotherapeutic activities of propolis and its updated underlying mechanisms. Taken together, propolis displays potent chemoprotective or anti-cancer effect due to the presence of various phytocomponents which contribute to pro-apoptotic, cytotoxic, anti-proliferative (cell cycle arrest), anti-metastatic, anti-invasive, anti-angiogenic and anti-genotoxic or anti-mutagenic properties along with antioxidant, immunomodulatory, and anti-inflammatory functions. Hence, propolis could be used as an adjuvant for treating various cancers along with standard chemotherapeutic drugs. However, many large-scale clinical studies are needed to justify such applications.

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Section: Chemopreventive On Prostate Cancer and Underlying Mechanismsmentioning

confidence: 99%

Section: Chemopreventive On Prostate Cancer and Underlying Mechanismsmentioning

confidence: 99%

Chemopreventive and Chemotherapeutic Effect of Propolis and Its Constituents: A Mini-review

et al. 2020

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“…In HepG2 cells, treatment with manuka honey and Doxorubicin had a synergistic effect on the induction of apoptosis [ 39 ]. In a different study, Algerian propolis potentiated the apoptotic effect of Doxorubicin in human pancreatic cancer cells [ 99 ].…”

Section: Resultsmentioning

confidence: 99%

Differential Apoptotic Effects of Bee Product Mixtures on Normal and Cancer Hepatic Cells

et al. 2023

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Most effective anticancer drugs normally generate considerable cytotoxicity in normal cells; therefore, the preferential activation of apoptosis in cancer cells and the reduction of toxicity in normal cells is a great challenge in cancer research. Natural products with selective anticancer properties used as complementary medicine can help to achieve this goal. The aim of the present study was to analyze the effect of the addition of bee products [propolis (PR) or royal jelly (RJ) or propolis and royal jelly (PR+RJ), 2–10%] to thyme (TH) and chestnut honeys (CH) on the differential anticancer properties, mainly the cytotoxic and pro-apoptotic effects, in normal and cancer hepatic cells. The cytotoxic effects of samples were analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (0–250 mg/mL) and the effects on apoptosis were analyzed using cell cycle analysis, TdT-dUTP terminal nick-end labeling (TUNEL) assay, DR5 (Death Receptor 5) and BAX (BCL-2-Associated X) activation, and caspases 8, 9, and 3 activities. Both honey samples alone and honey mixtures had no or very little apoptotic effect on normal cells. Antioxidant honey mixtures enhanced the apoptotic capacity of the corresponding honey alone via both extrinsic and intrinsic pathways. Of all the samples, chestnut honey enriched with 10% royal jelly and 10% propolis (sample 14, CH+10RJ+10PR) showed the highest apoptotic effect on tumor liver cells. The enrichment of monofloral honey with bee products could be used together with conventional anticancer treatments as a dietary supplement without side effects. On the other hand, it could be included in the diet as a natural sweetener with high added value.

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“…One of the most studied compounds of propolis is CAPE (20,21). It is known that there is a synergistic effect between propolis and anticancer agents, and that blast cells are directed to apoptosis and cell differentiation due to this effect (22,23). Various chemotherapeutic agents used for treatment can damage normal tissues and cells while healing damaged cells and tissues (24).…”

Section: Discussionmentioning

confidence: 99%

Effects of olive oil-based propolis, caffeic acid phenethyl ester, and methylprednisolone on differentiation of human acute myeloid leukemia cells

DOGAN¹,

Ozcimen²,

Silici³

2023

jrp

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Leukemia, originating from the hematopoietic system, is a malignant disease for the treatment of which various glucocorticoids are used. The use of natural products alongside conventional therapy has focused on increasing drug-drug interactions and reducing potential side effects. Based on the idea that propolis and caffeic acid phenethyl ester (CAPE) can stimulate cancer cells to differentiation and enhance the effect of chemotherapeutic drugs, we conducted this study. In this study, we investigated whether the synergistic effect of olive oil-based propolis and CAPE is effective with metylprednisolone (MP), which is used for supportive therapy in AML. In the study, OEP (Olive Oil-Based Propolis), CAPE, MP and their combinations were applied to HL -60 cells for 1-3 days. Then, CD11b, CD14 and CD68 antigens were detected on HL -60 cells using flow cytometry techniques to determine the cellular differentiations. As a result, HL -60 cells were found to be significantly directed to CD11b differentiation. OEP and CAPE will enhance the effect of chemotherapy by differentiating cells and ensure treatment completion by minimizing damage to normal tissues and cells.

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Algerian Propolis Potentiates Doxorubicin Mediated Anticancer Effect Against Human Pancreatic PANC-1 Cancer Cell Line through Cell Cycle Arrest, Apoptosis Induction and P-Glycoprotein Inhibition

Cited by 16 publications

References 0 publications

Chemopreventive and Chemotherapeutic Effect of Propolis and Its Constituents: A Mini-review

Chemopreventive and Chemotherapeutic Effect of Propolis and Its Constituents: A Mini-review

Differential Apoptotic Effects of Bee Product Mixtures on Normal and Cancer Hepatic Cells

Effects of olive oil-based propolis, caffeic acid phenethyl ester, and methylprednisolone on differentiation of human acute myeloid leukemia cells

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