Algibacter lectus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from green algae

Abstract

Three strains of the marine, gliding, pigmented, facultatively anaerobic, heterotrophic, Gram-negative bacteria were isolated from the green algae Acrosiphonia sonderi (Kütz) Kornm and Ulva fenestrata Ruprecht inhabiting the Sea of Japan. 16S rDNA sequence analysis indicated that the strains were members of the family Flavobacteriaceae, in which they occupied separate lineages. The predominant cellular fatty acids were i15 : 0, a15 : 0, i15 : 1, 15 : 0, 15 : 1ω6c, i15 : 0 3-OH and i17 : 0 3-OH. The DNA base compositions were 31–33 mol% G+C. Based on the phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, the novel bacteria should be placed in a novel taxon as Algibacter lectus gen. nov., sp. nov. with type strain KMM 3902T (=KCTC 12103T=DSM 15365T).

Formosa agariphila sp. nov., a budding bacterium of the family Flavobacteriaceae isolated from marine environments, and emended description of the genus Formosa

“…The major lipoquinone was MK-6. Polar lipids were determined as described previously (Nedashkovskaya et al, 2004b). The only phospholipid of KMM 3901 T and KMM 3962 was phosphatidylethanolamine, in accordance with the results reported for Formosa algae by Ivanova et al (2004).…”

Gaetbulibacter saemankumensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat sediment in Korea

“…Algibacter (Nedashkovskaya et al, 2004b), Winogradskyella (Nedashkovskaya et al, 2005a) and Bizionia (Nedashkovskaya et al, 2005b). Recently, three Gramnegative, slightly halophilic, yellow-pigmented bacterial strains, SMK-12 T , SMK-36 and SMK-45, were isolated from a tidal flat sediment in Korea.…”

“…The family Flavobacteriaceae forms an evolutionary lineage of descent within the domain Bacteria, together with the families Bacteroidaceae, Cytophagaceae, Sphingobacteriaceae, Spirosomaceae and several taxa unaffiliated to any family (Woese et al, 1985;Bernardet et al, 1996;Bernardet et al, 2002). The family Flavobacteriaceae accommodates bacteria that are motile by gliding or non-motile, chemo-organotrophic, Gram-negative and rod-shaped, and contain menaquinone-6 as the major isoprenoid quinone (Bernardet et al, 1996(Bernardet et al, , 2002Nedashkovskaya et al, 2004b). Currently, the family Flavobacteriaceae comprises more than 20 genera, including the recently described genera Formosa (Ivanova et al, 2004), Ulvibacter (Nedashkovskaya et al, 2004a),…”

“…Differential characteristics of Gaetbulibacter saemankumensis gen. nov., sp. nov. and phylogenetically related genera of the family Flavobacteriaceae Genera: 1, Gaetbulibacter gen. nov.; 2, Algibacter (data from Nedashkovskaya et al, 2004b); 3, Formosa (Ivanova et al 2004;Nedashkovskaya et al 2004bNedashkovskaya et al , 2005b; 4, Bizionia (Nedashkovskaya et al 2005b). +, Positive; 2, negative; V, variable; ND, not determined.…”

Psychroserpens mesophilus sp. nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae isolated from a young biofilm

“…The sequence was compared against the 16S rRNA gene sequences of strains belonging to P. burtonensis and of members of the genera Winogradskyella (Lau et al, 2005;Nedashkovskaya et al, 2005a), Gelidibacter (Bowman et al, 1997;Bowman & Nichols, 2005;Macián et al, 2002) and Subsaximicrobium (Bowman & Nichols, 2005), Subsaxibacter broadyi (Bowman & Nichols, 2005), Yeosuana aromativorans (Kwon et al, 2005b), Bizionia paragorgiae (Nedashkovskaya et al, 2005b), Formosa algae (Ivanova et al, 2004), Algibacter lectus (Nedashkovskaya et al, 2004) and Lacinutrix copepodicola (Bowman & Nichols, 2005). Closest sequence similarity was to P. burtonensis (95?0-95?6 %).…”