Algicide capacity of Paucibacter aquatile DH15 on Microcystis aeruginosa by attachment and non-attachment effects

Le, Ve Van; Ko, So-Ra; Kang, Mingyeong; Lee, Sang‐Ah; Oh, Hee‐Mock; Ahn, Chi‐Yong

doi:10.1016/j.envpol.2022.119079

Cited by 35 publications

(17 citation statements)

References 79 publications

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“…In line with our observations, previously characterized P. putida SK02 and Pseudomonas fluorescens SK09 showed interactive effect of cell‐to‐cell contact and algicidal substances during the cell lysis of the diatom, Stephanodiscus hantzschii (Jung et al, 2008; Kang et al, 2005). The recent findings of Van Le et al (2022) also reported efficient cyanolytic activity caused by Paucibacter aquatile DH15 against M. aeruginosa due to the combined effect of direct and indirect attacks. Hence, the present study suggests that the mode in which P. fluorescens BG‐E attacks the two studied Pseudanabaena species is strain‐specific, and these findings highlight the potential use of P. fluorescens BG‐E as a biological control agent to mitigate the proliferation of Pseudanabaena blooms in freshwaters.…”

Section: Discussionmentioning

confidence: 86%

“…Although the identification of an effective biological control agent is challenging, recognizing and isolating bacteria with the ability to lyse Microcystis sp. is considered possible (Van Wichelen et al, 2016;Yang et al, 2020). Accordingly, many studies have focused on cyanolytic bacteria that are isolated from freshwater as effective biological controlling agents against harmful and nuisance cyanobacterial blooms such as Microcystis (Gumbo et al, 2010;Guo et al, 2016;Liu et al, 2015Liu et al, , 2019.…”

Section: Shirani Manel Kumari Widana Gamagementioning

confidence: 99%

“…Additionally, some of these cyanolytic bacteria have the potential to degrade cyanotoxins such as MCs and suppress blooms instantaneously (Li et al, 2016). Consequently, considerable attention has been paid to heterotrophic bacteria in freshwater due to their potential as agents to control or terminate the excessive growth of cyanobacteria (Van Le et al, 2022; Wang et al, 2020; Zhang et al, 2019) and to degrade cyanotoxins (Chaffin et al, 2022; Idroos & Manage, 2018; Nájera et al, 2017). Although the identification of an effective biological control agent is challenging, recognizing and isolating bacteria with the ability to lyse Microcystis sp.…”

Section: Introductionmentioning

confidence: 99%

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A novel cyanolytic bacterium, Pseudomonas fluorescens BG‐E as a potential biological control agent for freshwater bloom‐forming cyanobacteria Pseudanabaena spp.

Wijesooriya

Masakorala

Gamage

2023

Journal of Phycology

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The majority of bacterial antagonists identified to date are active against Microcystis. Therefore, this study aimed to isolate and characterize novel cyanolytic bacterial strains antagonistic against bloom‐forming filamentous cyanobacteria. The bacterial strain BG‐E isolated from the Bandagiriya Wewa in Sri Lanka was identified as Pseudomonas fluorescens (MZ007859) based on the 16S rRNA gene sequencing. BG‐E showed 82% and 73% cyanolytic activity (CA) against Pseudanabaena sp. LW2 (MW288948) and Pseudanabaena lonchoides LW1 (MW288940), respectively, after 10 days of inoculation. The light microscopic images affirmed the complete disintegration in the filamentous structures of the tested Pseudanabaena species. The bacterial cell density of 15% v/v showed the CA with 95% and 89% cell lysis, respectively, in P. lonchoides and Pseudanabaena sp. LW2. Moreover, the results showed that >50% CA could be achieved by 0.100 and 1.00 (OD730) cell densities for these same species. The highest CA of the cell‐free supernatant of BG‐E against P. lonchoides and bacterial culture against Pseudanabaena sp. LW2 illustrated the species‐specific mode of action of BG‐E. Although BG‐E efficiently lysed the tested cyanobacterial species, the results of the MC‐biodegradation assay confirmed its inability to degrade MC‐LR cyanotoxin. Further, the BG‐E strain lacks the mlrABCD gene cluster which is known to be responsible for the enzymatic degradation of MCs. The overall findings highlighted the applicability of P. fluorescens BG‐E as a biological controlling agent to terminate blooms of freshwater filamentous cyanobacteria genus Pseudanabaena. The incorporation of cyanotoxin‐degrading heterotrophic bacteria is recommended as a means of controlling toxic Pseudanabaena blooms.

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Section: Discussionmentioning

confidence: 86%

Section: Shirani Manel Kumari Widana Gamagementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel cyanolytic bacterium, Pseudomonas fluorescens BG‐E as a potential biological control agent for freshwater bloom‐forming cyanobacteria Pseudanabaena spp.

Wijesooriya

Masakorala

Gamage

2023

Journal of Phycology

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“…Approximately 30% of bacteria reported are suggested to be direct algicidal bacteria, whereas the remaining 70% are indirect ones [ 20 ]. Algicidal bacteria displaying both mechanisms have been rarely identified [ 21 ]. Both modes of algicidal bacteria and their active algicidal chemicals are considered as effective agents for controlling HCBs.…”

Section: Introductionmentioning

confidence: 99%

Discovery of a High-Efficient Algicidal Bacterium against Microcystis aeruginosa Based on Examinations toward Culture Strains and Natural Bloom Samples

Zhang

Xie

Zhang³

et al. 2023

Toxins

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Harmful cyanobacterial blooms occur worldwide and pose a great threat to aquatic ecosystems and public health. The application of algicidal bacteria represents an eco-friendly strategy for controlling harmful cyanobacterial blooms; thus, searching for a high efficiency of algicidal bacteria has been becoming an important and continuous task in science. Herein, we identified a bacterial strain coded Streptomyces sp. HY with a highly algicidal activity, and investigated its algicidal efficiency and mechanism against Microcystis aeruginosa. The strain HY displayed high algicidal activity toward Microcystis aeruginosa cells, with a removal rate of 93.04% within 2 days via indirect attack. Streptomyces sp. HY also showed the ability to lyse several genera of cyanobacterial strains, including Dolichospermum, Pseudanabaena, Anabaena, and Synechocystis, whereas it showed a minor impact on the green alga Scenedesmus obliquus, demonstrating its selectivity specially for targeting cyanobacteria. Its algicidal mechanism involved damages to the photosynthesis system, morphological injury of algal cells, oxidative stress, and dysfunction of the DNA repair system. Furthermore, HY treatment reduced the expression levels of genes (mcyB and mcyD) related to microcystin biosynthesis and decreased the total content of microcystin-leucine-arginine by 79.18%. Collectively, these findings suggested that the algicidal bacteria HY is a promising candidate for harmful cyanobacterial bloom control.

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“…Bacterial control of natural algal blooms by the Roseobacter lineage prevalent in algal blooms dominated by dinoflagellates and E. huxleyi was described in one of the early studies by Gonzalez and co-authors 25 . In freshwater systems that function as sources of drinking water for human use, it is essential to reach a detailed understanding of the control of harmful algal blooms (HAB) by bacteria [26][27][28] . The mesocosm experiments provided a possibility of modeling algal-microbial interactions in larger volumes and conditions closer to the natural environment than can be obtained in lab cultures [29][30][31][32] .…”

Section: Introductionmentioning

confidence: 99%

Dynamics of associated microbiomes during algal bloom development: to see and to be seeing

Meirkhanova,

Zhumakhanova,

Len

et al. 2023

Preprint

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Our understanding of the interactions between bacteria and phytoplankton in the freshwater phycosphere, including the development of algal blooms, is very limited. To identify the taxa and compositional variation within microbial communities, we performed 16S rRNA amplicon sequencing research on samples collected weekly through summer from mesocosms that differed in temperature and mixing regimes. We investigated, for the first time, the abundance diversity of microalgae, including Chlorophyta, Cryptophyta, and Cyanobacteria species, using visualization-based FlowCAM analysis and classification of microbial communities to species level by nanopore next-generation sequencing. We found that nanopore metagenomics, in parallel with complementary imaging flow cytometry, can depict the fine temporal dynamics of microbiomes associated with visually identified Microcystis morphospecies, Chlorophyta, and Cryptophyta during algal bloom development. Our results showed that the temporal characteristics of microbiomes combined with a visual approach may be a key tool to predict the metacommunity structure and dynamics of algal blooms in response to anthropogenic effects and climate change.

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Algicide capacity of Paucibacter aquatile DH15 on Microcystis aeruginosa by attachment and non-attachment effects

Cited by 35 publications

References 79 publications

A novel cyanolytic bacterium, Pseudomonas fluorescens BG‐E as a potential biological control agent for freshwater bloom‐forming cyanobacteria Pseudanabaena spp.

A novel cyanolytic bacterium, Pseudomonas fluorescens BG‐E as a potential biological control agent for freshwater bloom‐forming cyanobacteria Pseudanabaena spp.

Discovery of a High-Efficient Algicidal Bacterium against Microcystis aeruginosa Based on Examinations toward Culture Strains and Natural Bloom Samples

Dynamics of associated microbiomes during algal bloom development: to see and to be seeing

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