Alginate-based hydrogels with improved adhesive properties for cell encapsulation

Sarker, Bapi; Rompf, Julia; Silva, Raquel; Lang, Nadine; Detsch, Rainer; Kaschta, Joachim; Fabry, Ben; Boccaccını, Aldo R.

doi:10.1016/j.ijbiomac.2015.03.061

Cited by 134 publications

(94 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result of the cell viability kinetic is consistent with similar studies done with human osteoblast-like MG-63 cells, which were encapsulated in ADA-GEL hydrogel bioplotted structures [27] as well as in microcapsules [41]. Bioplotted ADA-GEL structures loaded with HCT116 cells also showed high cell viabilities after the plotting process [42].…”

Section: Resultssupporting

confidence: 85%

“…This result is in contradiction to studies using PCL and cell-loaded pure alginate as in this case no influence or interaction of the immobilised cells with the PCL structure was reported [36]. The results could be explained by favourable properties of the ADA-GEL in comparison to pure alginate considering a faster degradation, influencing cell mobility, and cell-material interaction [23,41]. …”

Section: Resultsmentioning

confidence: 63%

See 1 more Smart Citation

Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering

et al. 2016

Self Cite

View full text Add to dashboard Cite

Hydrogel optimisation for biofabrication considering shape stability/mechanical properties and cell response is challenging. One approach to tackle this issue is to combine different additive manufacturing techniques, e.g., hot-melt extruded thermoplastics together with bioplotted cell loaded hydrogels in a sequential plotting process. This method enables the fabrication of 3D constructs mechanically supported by the thermoplastic structure and biologically functionalised by the hydrogel phase. In this study, polycaprolactone (PCL) and polyethylene glycol (PEG) blend (PCL-PEG) together with alginate dialdehyde gelatine hydrogel (ADA-GEL) loaded with stromal cell line (ST2) were investigated. PCL-PEG blends were evaluated concerning plotting properties to fabricate 3D scaffolds, namely miscibility, wetting behaviour and in terms of cell response. Scaffolds were characterised considering pore size, porosity, strut width, degradation behaviour and mechanical stability. Blends showed improved hydrophilicity and cell response with PEG blending increasing the degradation and decreasing the mechanical properties of the scaffolds. Hybrid constructs with PCL-PEG blend and ADA-GEL were fabricated. Cell viability, distribution, morphology and interaction of cells with the support structure were analysed. Increased degradation of the thermoplastic support structure and proliferation of the cells not only in the hydrogel, but also on the thermoplastic phase, indicates the potential of this novel material combination for biofabricating 3D tissue engineering scaffolds.

show abstract

Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 63%

Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is clear from the SEM images that ADA-GEL promoted cell migration due to its lower polymeric mesh density and the release of gelatin from the matrix(11). Similar outcome has been observed for osteosarcoma cells in ADA-GEL matrix(6,12). The improved cell attachment could be explained by the gelatin content of the ADA-GEL providing cell adhesion motifs in comparison to pure alginate.…”

supporting

confidence: 64%

“…3D environments are achieved by embedding cells in a hydrogel (12). In general, hydrogels from naturally occurring biopolymers such as alginate and gelatin are an interesting class of biomaterials widely used in biomedical applications because of their biocompatibility and biodegradability (13).…”

Section: Introductionmentioning

confidence: 99%

Biofabrication of 3D Alginate-Based Hydrogel for Cancer Research: Comparison of Cell Spreading, Viability, and Adhesion Characteristics of Colorectal HCT116 Tumor Cells

Ivanovska

Zehnder

Lennert

et al. 2016

Tissue Engineering Part C: Methods

Self Cite

View full text Add to dashboard Cite

Hydrogels are an important class of biomaterials as they could mimic the extracellular matrix (ECM). Among the naturally occurring biopolymers, alginate and gelatin are extensively used for many biomedical applications. For developing biofabrication constructs as three-dimensional (3D) cell culture models, realistic imaging of cell spreading and proliferation inside the hydrogels represents a major challenge. Therefore, we aimed to establish a system that can mimic the structural architecture, composition, and biological functions of the ECM for cancer research approaches. For this, we compared the cell behavior of human colon cancer HCT116 cells in two biofabricated hydrogels as follows: pure alginate and cross-linked alginate-gelatin (ADA-GEL) matrixes. Our data indicate that cells from the ADA-GEL matrix showed highest proliferation and cellular networks through the material. Analyzing the mRNA expression of several integrins of cells cultured inside of the matrix, we showed that mRNA expression of integrin subunits differed based on the cell focal adhesion characteristics. Furthermore, we showed that recultured ADA-GEL immobilized cells do not differ from parental HCT116 cells regarding migration and proliferation capabilities. Comparing adhesion and other phenotypic characteristics of HCT116 tumor cells, we suggest that ADA-GEL hydrogel is a more suitable 3D system than pure alginate and seems to optimally mimic the physiological behavior of the tumor microenvironment. For the first time, we present a functional 3D hydrogel construct for colon cancer cells, which are supporting their physiological cell attachment, spreading, and viability. We strongly believe that it will be applicable as a suitable in vitro 3D tumor model to study different aspects of tumor cell behavior.

show abstract

“…Partrap et al [18], for example utilized self-assembled micelles formed from cetyl trimethyl ammonium bromide (CTAB) surfactant to produce a template for alginate gelation resulting in hydrogels with controllable pore size ranging from 30 to 160µm. Alginate's lack of inherent cell adhesive properties, is a major limitation in scaffolding applications leading to arrested development of anchorage-dependent cells and tissue formation [19]. Attempts to resolve this shortcoming have involved modifying alginate or the finished scaffold with extra cellular matrix (ECM) proteins, such as fibronectin, vitronectin, laminin, and collagen for binding with cell adhesive receptors.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Production and in vitro evaluation of macroporous, cell-encapsulating alginate fibres for nerve repair

Lin

Wang

Wertheim

et al. 2017

Materials Science and Engineering: C

View full text Add to dashboard Cite

The prospects for successful peripheral nerve repair using fibre guides are considered to be enhanced by the use of a scaffold material, which promotes attachment and proliferation of glial cells and axonal regeneration. Macroporous alginate fibers were produced by extraction of gelatin particle porogens from wet spun fibers produced using a suspension of gelatin particles in 1.5% w/v alginate solution. Gelatin loading of the starting suspension of 40.0, 57.0, and 62.5% w/w resulted in gelatin loading of the dried alginate fibers of 16, 21, and 24% w/w respectively. Between 45 and 60% of the gelatin content of hydrated fibers was released in 1 h in distilled water at 37°C, leading to rapid formation of a macroporous structure. Confocal laser scanning microscopy (CLSM) and image processing provided qualitative and quantitative analysis of mean equivalent macropore diameter (48-69 µm), pore size distribution, estimates of maximum porosity (14.6%) and pore connectivity.CLSM also revealed that gelatin residues lined the macropore cavities and infiltrated into the body of the alginate scaffolds, thus, providing cell adhesion molecules, which are potentially advantageous for promoting growth of glial cells and axonal extension. Macroporous alginate fibres encapsulating nerve cells [primary rat dorsal root ganglia (DRGs)] were produced by wet spinning alginate solution containing dispersed gelatin particles and DRGs. Marked outgrowth was evident over a distance of 150 µm at day 11 in cell culture, indicating that pores and channels created within the alginate hydrogel were providing a favourable environment for neurite development. These findings indicate that macroporous alginate fibres encapsulating nerve cells may provide the basis of a useful strategy for nerve repair.

show abstract

Alginate-based hydrogels with improved adhesive properties for cell encapsulation

Cited by 134 publications

References 28 publications

Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering

Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering

Biofabrication of 3D Alginate-Based Hydrogel for Cancer Research: Comparison of Cell Spreading, Viability, and Adhesion Characteristics of Colorectal HCT116 Tumor Cells

Production and in vitro evaluation of macroporous, cell-encapsulating alginate fibres for nerve repair

Contact Info

Product

Resources

About