Alginate-Collagen Fibril Composite Hydrogel

Baniasadi, Mahmoud; Minary‐Jolandan, Majid

doi:10.3390/ma8020799

Cited by 91 publications

(54 citation statements)

References 68 publications

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“…Similar results were observed with cell aggregates, where the presence of Col increased the migration length and area. This behavior can be attributed to presence of the integrin interaction site Arginine, Glycine und Aspartate, (RGD sequence) naturally contained in Col inducing cell adhesion [38]. A direct correlation of cell adhesion and RGD density was shown also for electro spun HA functionalized with RGD peptide by Kim et al [39].…”

Section: Discussionmentioning

confidence: 90%

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

Schwab

Hélary

Richards

et al. 2020

Preprint

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Biofabrication is providing scientists and clinicians the ability to produce engineered tissues with desired shapes, chemical and biological gradients. Typical resolutions achieved with extrusion-based bioprinting are at the macroscopic level. However, for capturing the fibrillar nature of the extracellular matrix (ECM), it is necessary to arrange ECM components at smaller scales, down to the sub-micron and the molecular level. In this study, we introduce a (bio)ink containing hyaluronan (HA) as tyramine derivative (THA) and collagen (Col). Similarly to other connective tissues, in this (bio)ink Col is present in fibrillar form and HA as viscoelastic space filler. THA was enzymatically crosslinked under mild conditions allowing simultaneous Col fibrillogenesis, thus achieving a homogeneous distribution of Col fibrils within the viscoelastic HA-based matrix. THA-Col composite displayed synergistic properties in terms of storage modulus and shear-thinning, translating into good printability. Shear-induced alignment of the Col fibrils along the printing direction was achieved and quantified via immunofluorescence and second harmonic generation. Cell-free and cell-laden constructs were printed and characterized, analyzing the influence of the controlled microscopic anisotropy on cell behavior and chondrogenic differentiation. THA-Col showed cell instructive properties modulating hMSC adhesion, morphology and sprouting from spheroids stimulated by the presence and the orientation of Col fibers. Actin filament staining showed that hMSCs embedded into aligned constructs displayed increased cytoskeleton alignment along the fibril direction. Based on gene expression of cartilage/bone markers and matrix production, hMSCs embedded into the bioink displayed chondrogenic differentiation comparable or superior to standard pellet culture by means of proteoglycan production (Safranin O staining and proteoglycan quantification) as well as increase in cartilage related genes. The possibility of printing matrix components with control over microscopic alignment brings biofabrication one step closer to capturing the complexity of native tissues.

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Section: Discussionmentioning

confidence: 90%

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

Schwab

Hélary

Richards

et al. 2020

Preprint

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“…The tan d values were approximately the same for all gels with the exception of the glutaric anhydride and maleic anhydride gels. 46 The overall trends were similar but it was not possible to directly compare the values obtained with the different techniques they utilised. Kaufman et al compared nanoindentation values with macroscale compression tests for pHEMA gels and found a greater discrepancy with the most compliant (i.e.…”

Section: Resultsmentioning

confidence: 99%

Using the hydrolysis of anhydrides to control gel properties and homogeneity in pH-triggered gelation

et al. 2015

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The pH of an aqueous solution of a low molecular weight gelator can be adjusted through the hydrolysis of a number of anhydrides to the corresponding acids. The rate of hydrolysis and hence of pH change can be used to control the rate of gel formation. This rate does not affect the primary assembly of the low molecular weight gelator, but does affect the mechanical properties of the resulting gels, as well as the homogeneity and reproducibility of the gels. The mechanical properties are compared by both rheology and dynamic nanoindentation.

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“…This may lead to the pooling of fibrin gel. Furthermore as fibrin hydrogel is reported to have a storage modulus of ∼9.5 kPa, while acidified calcium carbonate alginate gels have been reported to have moduli up to ∼1 kPa, it may be expected that alginate is more likely to permanently deform and be ejected due to cardiac contraction. One potential explanation for the reported increased alginate retention could be due to the swelling nature of the alginate used .…”

Section: Discussionmentioning

confidence: 99%

An in vitro investigation to assess procedure parameters for injecting therapeutic hydrogels into the myocardium

et al. 2016

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Localized delivery of stem cells is potentially a promising therapeutic strategy for regenerating damaged myocardium. Many studies focus on limiting the biologic component of cell loss, but few address the contribution of mechanical factors. This study investigates optimal parameters for retaining the largest volume of cell loaded hydrogels post intramyocardial injection, without compromising cell viability. In vitro, hydrogel was injected into porcine hearts using various needle designs. Hydrogel retention and distribution pattern was then determined. The two most promising needles were then investigated to understand the effect of needle geometry on stem cell viability. The needle to best impact cell viability was then used to investigate the effect of differing hydrogels on retention and distribution. Three-dimensional experimental modeling revealed needles with smaller diameter's to have greater poloxamer 407 hydrogel retention. No difference in retention existed among various needle designs of similar gauge, despite differences in bolus geometries. When hMSC's, embedded in fibrin hydrogel, were injected through helical and 26G bevel needles no difference in the percent of live cells was seen at 48 h. However, the helical group had almost half the metabolic activity of the 26G bevel group at both time points, and had a significant decline in the percent of live cells from 24 to 48 h. Varying gel type resulted in significantly more alginate being retained in the tissue in comparison to fibrin or poloxamer hydrogels. In conclusion, mechanical properties of injected hydrogels, and the diameter of the needle used, highly influences the volume of hydrogel retained. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2618-2629, 2017.

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Alginate-Collagen Fibril Composite Hydrogel

Cited by 91 publications

References 68 publications

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

Using the hydrolysis of anhydrides to control gel properties and homogeneity in pH-triggered gelation

An in vitro investigation to assess procedure parameters for injecting therapeutic hydrogels into the myocardium

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