Alginate-immobilized lipase by electrostatic extrusion for the purpose of palm oil hydrolysis in lecithin/isooctane system

Knežević, Zorica; Bobic, Svetlana; Milutinović, A.; Obradović, Bojana; Mojović, Ljiljana; Bugarski, Branko

doi:10.1016/s0032-9592(02)00085-7

Cited by 77 publications

(51 citation statements)

References 25 publications

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“…The same interpretation can be applied to the MBA content in hydrogels: if the hydrogels of the same acid content and different MBA contents are compared (i.e., 90/10/2 and 90/10/4) it can be concluded that an increase in crosslinking agent content leads to the decrease of the lipase SA. The immobilized lipase activity yield was in the range between 31.0 and 86.5 %, which was comparable or much higher than the results achieved by other authors and by applying some different immobilization methods [30][31][32]. The highest amount of immobilized lipase and SA were achieved for hydrogels 85/15/2, 90/10/ 2, and 90/10/4.…”

Section: Sem and Ifm Analysessupporting

confidence: 74%

Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions

et al. 2012

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Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5°C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25°C.

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Section: Sem and Ifm Analysessupporting

confidence: 74%

Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions

et al. 2012

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“…Increasing the concentration of CuSO 4 and other cationic solutions had a negative effect on immobilization yield. It was earlier reported that increasing of the alginate or CuSO 4 concentration limits the substrate transfer into the alginate bead (Knezevic et al 2002;Teerapatsakul et al 2008). Also in the immobilisation of Candida rugosa lipase, increasing the alginate concentration decreased immobilisation yield, but increasing the concentration of the cross-linking agent, CaCl 2 had little effect (Won et al 2005).…”

Section: Resultsmentioning

confidence: 88%

Assessing the tolerance of immobilized laccase from a salt-tolerant strain of Trichoderma viride Pers NFCCI-2745 to heavy metal ions, detergents and copper chelating agents

Divya

Prasanth

Sadasivan

2014

Int. J. Environ. Sci. Technol.

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The biotreatment of industrial effluents that contain heavy metals, detergents and metal chelating agents represents a great concern to industries as these compounds are either toxic to microbes or they act as noncompetitive inhibitors/denaturing agents for several enzymes. So, it is essential to know the effect of various agents usually present in the treatment plant on activity of the enzyme, for extending its applications in in situ treatment processes. This study describes the immobilization conditions of laccase and analysis of the sensitivity of immobilized laccase from Trichoderma viride Pers NFCCI-2745 to heavy metals, detergent and copper chelating agents. The concentrations and combinations of cations influenced the activity of immobilized enzyme and its yield. The immobilized Cu-Ba alginate enzyme showed comparatively higher activity compared to the Cu-alginate enzyme. Most of the metal ions tested enhanced laccase activity at lower concentration. Immobilized laccase retained more than 70 % of its activity even in the presence of 20 mM copper chelating agents like EDTA and sodium thioglycolic acid. Sodium azide, on the other hand, inhibited 80 % of the activity of all the beads even at a very low concentration of 0.5 mM. Cu-Ba and Cu-Ni alginate enzyme exhibited comparatively higher tolerance than Cualginate beads to EDTA and sodium thioglycolate. Tween 20 and cetyl trimethyl ammonium bromide significantly increased the activity of all the beads. These properties of the immobilized laccase may be aptly considered and suitably utilized in treating phenolic effluents that may contain heavy metals, detergents and certain copper chelating agents which may otherwise pose a threat to enzymatic activity.

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“…This is because the anion alginate crosslinked with Ca 2+ ions, the increase in the concentration of alginate cross allegedly makes connective tissue more and more. The amount of connective tissue makes the rate of diffusion to cross into or out through the smaller matrix [8,13,14]. The Candida rugosa lipase (56 kDa) can not diffuse out the matrix, so that the value loading is high.…”

Section: Optimization Ofcandidarugosalipaseimmobilizedinca-alginate Mmentioning

confidence: 99%

Esterification of Glucose by Coconut Oil Fatty Acids Using Immobilized CandidarugosaLipaseE.C.3.1.1.3. inCa-alginate Matrix

Handayani¹

2012

IOSRJAC

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Alginate-immobilized lipase by electrostatic extrusion for the purpose of palm oil hydrolysis in lecithin/isooctane system

Cited by 77 publications

References 25 publications

Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions

Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions

Assessing the tolerance of immobilized laccase from a salt-tolerant strain of Trichoderma viride Pers NFCCI-2745 to heavy metal ions, detergents and copper chelating agents

Esterification of Glucose by Coconut Oil Fatty Acids Using Immobilized CandidarugosaLipaseE.C.3.1.1.3. inCa-alginate Matrix

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