Alginate Matrix of MDA-MB-231 Breast Cancer Cell 3D Culturing Alters CD44 and CD24 mRNA Levels and Induces ALDH1 Expression

Souza, Layane Duarte e; Ferraz, Elisa Raquel Anastácio; Salviano, Ísis; Fonseca, A S; Felzenzswalb, Israel; Mencalha, André Luiz

doi:10.17265/1934-7391/2017.05.002

Cited by 2 publications

(1 citation statement)

References 45 publications

(78 reference statements)

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“…Alginate has been explored for cell encapsulation in many studies due to thermally stable gelation under cold conditions and ion exchange reactions [138]. Alginate hydrogels have been explored for the 3D encapsulation of breast cancer cells [139], human leukemia cells [140], and enrichment of cancer stem cells from human hepatocarcinoma cells [141,142]. Recently a hybrid hydrogel of Alginate and Matrigel was investigated with breast cancer cell lines MDA-MB-231 to design 3D material to be successfully used as a substrate for breast cancer cell culture [143].…”

Section: Alginatementioning

confidence: 99%

3D Modeling of Epithelial Tumors—The Synergy between Materials Engineering, 3D Bioprinting, High-Content Imaging, and Nanotechnology

Trivedi

et al. 2021

IJMS

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The current statistics on cancer show that 90% of all human cancers originate from epithelial cells. Breast and prostate cancer are examples of common tumors of epithelial origin that would benefit from improved drug treatment strategies. About 90% of preclinically approved drugs fail in clinical trials, partially due to the use of too simplified in vitro models and a lack of mimicking the tumor microenvironment in drug efficacy testing. This review focuses on the origin and mechanism of epithelial cancers, followed by experimental models designed to recapitulate the epithelial cancer structure and microenvironment, such as 2D and 3D cell culture models and animal models. A specific focus is put on novel technologies for cell culture of spheroids, organoids, and 3D-printed tissue-like models utilizing biomaterials of natural or synthetic origins. Further emphasis is laid on high-content imaging technologies that are used in the field to visualize in vitro models and their morphology. The associated technological advancements and challenges are also discussed. Finally, the review gives an insight into the potential of exploiting nanotechnological approaches in epithelial cancer research both as tools in tumor modeling and how they can be utilized for the development of nanotherapeutics.

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Section: Alginatementioning

confidence: 99%

3D Modeling of Epithelial Tumors—The Synergy between Materials Engineering, 3D Bioprinting, High-Content Imaging, and Nanotechnology

Trivedi

et al. 2021

IJMS

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Programmed death-ligand 1 expression in human cancer three-dimensional cell culture models

Hudson

Cross

Jordan-Mahy

et al. 2022

Preprint

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Programmed death-ligand 1 (PD-L1) expression is a survival mechanism employed by tumours to mediate immune evasion and tumour progression. PD-1/PD-L1-targeted therapies have revolutionised the cancer therapy landscape due to their ability to promote durable anti-tumour immune responses in select patients with advanced cancers. However, some patients are unresponsive, hyper-progressive or develop resistance. Better characterisation of the 3D architecture of solid tumours by utilising 3D cell culture could provide an environment that more closely recapitulates in vivo human tumours for investigating tumour-intrinsic PD-L1 signalling and immunotherapy responses. Here we investigated whether PD-L1 expression by human breast, prostate and colorectal cancer cell lines altered in 3D cell culture models compared to their 2D monolayer counterparts. We found that PD-L1 expression changed in 3D-cultured cancer cells when compared to 2D-cultured cells. Additionally, the expression of immunological markers, PD-1, PD-L2, CD44, DR4, DR5, Fas, and HLA-ABC were assessed in 3D cell culture and compared to their expression in 2D. These markers were also altered in 3D compared to 2D-cultured cells, highlighting the importance of utilising 3D models which may better able the investigation of tumour-intrinsic PD-L1 signalling, responses to PD-1/PD-L1-targeted therapy, and combination therapies.

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Alginate Matrix of MDA-MB-231 Breast Cancer Cell 3D Culturing Alters CD44 and CD24 mRNA Levels and Induces ALDH1 Expression

Cited by 2 publications

References 45 publications

3D Modeling of Epithelial Tumors—The Synergy between Materials Engineering, 3D Bioprinting, High-Content Imaging, and Nanotechnology

3D Modeling of Epithelial Tumors—The Synergy between Materials Engineering, 3D Bioprinting, High-Content Imaging, and Nanotechnology

Programmed death-ligand 1 expression in human cancer three-dimensional cell culture models

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