Alginate microbeads as inoculant carriers for plant growth-promoting bacteria

Bashan, Yoav; Hernández, Juan-Pablo; Leyva, Luis A.; Bacilio, Macario

doi:10.1007/s00374-002-0481-5

Cited by 229 publications

(188 citation statements)

References 42 publications

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“…It is also observed that the bacteria tend to occupy the pores present in the gel matrix (Bashan, 1986b). Although a variety of biomaterials are available, alginate is preferred for encapsulation of microbial cells by several workers (Bashan et al, 2002) and the cell density within alginate beads can be maintained as high as 10 10 -10 11 CFU/g of bead (Chang et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…A number of recent studies carried out on the application of immobilized, mainly entrapped and encapsulated, cells of microorganisms undoubtedly shows their advantages over traditionally used free cell inoculation in processes such as rock phosphate solubilization and nitrogen fixation (Bashan et al, 2002;Fenice et al, 2000). The potential modification in formulation described here will be useful to agro industry.…”

Section: A-cmentioning

confidence: 93%

“…Immobilization of microbial cells into polymer matrix has proved to be advantageous over direct soil inoculation (Cassidy et al, 1996). The main goals of encapsulation of PGPB is to protect them from harsh soil environment, reduce microbial competition and release them gradually to facilitate colonization of plant roots (Bashan et al, 2002;Vassilev et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

Young¹,

Rekha²,

Lai³

et al. 2006

Biotech & Bioengineering

154

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The key to achieving successful, reproducible results following the introduction of beneficial microbes into soil relies on the survival rate of the inoculated bacteria in a heterogeneous soil environment and hence an improved encapsulation method was developed. Owing to the constraints associated with the inoculum formulation, in this study, encapsulation of a plant growth promoting bacteria (PGPB) isolate Bacillus subtilis CCpg104 was attempted with alginate by enriching the bead microenvironment with humic acid. High viability of the encapsulated bacteria was observed with minimum cell loss upon storage for 5 months. Steady and constant cell release from the bead was observed for 1 week at different pH. Encapsulated cells remained active as evidenced by their ability to solubilize calcium phosphate in vitro. Successful plant growth promotion of lettuce by the encapsulated bacteria under gnotobiotic and sterile environment was also achieved. Feasibility of this improved encapsulation technique is mainly due to the dual benefits of humic acid to microbe and plant and its chemical properties allowing an easy mixing with alginate without interfering in the formation of the alginate gel beads by cross-linking with Ca 2þ ions. Thus, the encapsulation method described in this study can be effectively used to protect the PGPB inoculum from adverse conditions of the soil for their successful establishment in the rhizosphere. ß

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Section: Discussionmentioning

confidence: 99%

Section: A-cmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

Young¹,

Rekha²,

Lai³

et al. 2006

Biotech & Bioengineering

154

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“…식물 근권 토양에서 추출한 g-DNA를 template로 사용하였으며, 1차 PCR은 DNA template 2 µl, 27F 1 µl, 515R 1 µl, 10X taq polymerase buffer 2 µl, 2.5 mM dNTP 2.5 µl, Ex-taq polymerase (TaKaRa Shuzo Co., Japan) 0.125 µl를 섞고 3차 증류수를 추가하여 최종부피를 25 µl로 보정하였다. Azospirillum brasilense가 18일 배양 후 토마토 유묘를 비접종 대조군에 비해 shoot 길이 생장을 약 30% 증가시킨 결과와 비교 하여 상당히 높다 (Bashan et al, 2002). 비록 대상 식물 종과 환 경조건이 다르지만 alginate에 고정한 Pseudomonas fluorescens BAM-4와 Burkholderia cepacia BAM-12가 밀의 shoot 길이 생 장을 비접종 대조군보다30-40% 정도 증가시킨 결과보다 우수 하였으며 (Minaxi, 2011), 상추(Lectuca sativa)를 대상으로 humic acid를 첨가한 alginate에 Pseudomonas putida CC-FR2-4를 고 정화하여 접종했을 때 현탁액 접종보다 shoot와 뿌리 길이가 5-10% 정도로 생장이 증가한 결과와 유사하였다 (Rekha et al, 2007).…”

Section: 세균군집 Dna 추출 및 증폭unclassified

“…이제까지 고정화 PGPR 균주에 의한 식물생장촉진 연구는 많 았지만 균주의 근권 잔류에 대해서는 조사하지 않거나 (Rekha et al, 2007;Schoebitz et al, 2014) 고정화 담체에서 방출되는 생 균수만을 측정한 경우가 대부분이었으며 (Bashan et al, 2002;Minaxi, 2011 …”

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Growth Promotion of Tomato by Application of Immobilized Arthrobacter woluwensis ED in Alginate Beads

Kwon¹,

Song

2014

The Korean Journal of Microbiology

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In order to increase the persistence of plant growth promoting rhizobacteria (PGPR) in rhizpsphere soil, the growth of tomato was examined after the application of Arthrobacter woluwensis ED immobilized in alginate bead, which was known as PGPR. When tomato seedlings were treated with A. woluwensis ED of 1 × 10 6 cells g soil -1 and incubated for 30 days in a plant growth chamber, the shoot length, root length, fresh weight and dry weight of the grown tomato plants treated with the suspended inoculants significantly increased by 36.2, 59, 51.1, and 37.5%, respectively compared to those of the uninoculated control. The treatment of the immobilized bacteria increased those by 42, 67.4, 62.5, and 60.4%, respectively compared to those of the uninoculated control. Therefore, the enhancement of tomato growth by the treatment of the immobilized bacteria was higher than those by the suspended inoculants. The effects of the inoculation on indigenous bacterial community and the fate of the inoculated bacteria were monitored by denaturing gradient gel electrophoresis analysis. The DNA band intensity of A. woluwensis ED in the tomato rhizosphere treated with the suspended inoculants continuously decreased after the inoculation, but the band intensity in the tomato rhizosphere soils treated with the immobilized inoculants showed the maximum at 1 week after inoculation and the decreasing rate was less than that of the suspended inoculants, which indicated the longer maintenance of the immobilized bacteria at rhizosphere soils. Therefore, encapsulation of PGPR in alginate beads may be more effective than liquid inoculant for the plant growth promotion and survival of PGPR at plant rhizosphere.

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Plant Probiotics for Nutrient Acquisition by Agriculturally Important Grasses: A Comprehensive Review of the Science and the Application

Swift

Denton

Melino

2019

Annual Plant Reviews Online

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Intensification of agricultural land and the overuse of inorganic fertilisers has led to soil acidification, depletion of organic matter, and environmental pollution. Approaches to protect soil health, including the enhancement of microbial diversity, are integral to improving crop productivity and food security. Metagenomics has rapidly improved our understanding of soil microbial diversity and function, while genetic techniques have helped to dissect the complex signal exchange between microorganisms and plants. This article presents and evaluates reported beneficial effects of plant growth‐promoting bacteria (PGPB), focussing on those capable of mobilising or solubilising nutrients and/or stimulating plant growth when applied to agriculturally important grasses. The agricultural industry has capitalised on these scientific advancements, generating microbial formulations for specific crop responses. However, scientific methodologies must be applied in order to overcome inherent limitations of many PGPBs including their inability to be cultured, their poorly defined or multiple modes of action, a low level of integration with the crop partner, and an unpredictability in translating beneficial plant responses to the field. Novel approaches such as engineered rhizospheres, enhancing endophytic systems, cereal nodule development, and the use of inoculant consortiums will be necessary to sustain growth in the biofertiliser industry.

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Alginate microbeads as inoculant carriers for plant growth-promoting bacteria

Cited by 229 publications

References 42 publications

Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

Growth Promotion of Tomato by Application of Immobilized Arthrobacter woluwensis ED in Alginate Beads

Plant Probiotics for Nutrient Acquisition by Agriculturally Important Grasses: A Comprehensive Review of the Science and the Application

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