Alginate Microspheres Elicit Innate M1-Inflammatory Response in Macrophages Leading to Bacillary Killing

Vaghasiya, Kalpesh; Eram, Aisha; Sharma, Ankur; Ray, Eupa; Adlakha, Suneera; Verma, Rajkumar

doi:10.1208/s12249-019-1458-0

Cited by 11 publications

(4 citation statements)

References 41 publications

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“…Ultimately, pure alginate microsphere stimulates M1 polarization with a high level of pro‐inflammatory cytokines secretion. [ 192 ]…”

Section: Influence Of Polysaccharide‐coated Particles On Macrophagementioning

confidence: 99%

The Influence of Polysaccharides‐Based Material on Macrophage Phenotypes

Bratlie

2021

Macromolecular Bioscience

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Macrophage polarization is a key factor in determining the success of implanted tissue engineering scaffolds. Polysaccharides (derived from plants, animals, and microorganisms) are known to modulate macrophage phenotypes by recognizing cell membrane receptors. Numerous studies have developed polysaccharide‐based materials into functional biomaterial substrates for tissue regeneration and pharmaceutical application due to their immunostimulatory activities and anti‐inflammatory response. They are used as hydrogel substrates, surface coatings, and drug delivery carriers. In addition to their innate immunological functions, the newly endowed physical and chemical properties, including substrate modulus, pore size/porosity, surface binding chemistry, and the mole ratio of polysaccharides in hybrid materials may regulate macrophage phenotypes more precisely. Growing evidence indicates that the sulfation pattern of glycosaminoglycans and proteoglycans expressed on polarized macrophages leads to the changes in protein binding, which may alter macrophage phenotype and influence the immune response. A comprehensive understanding of how different types of polysaccharide‐based materials alter macrophage phenotypic changes can be beneficial to predict transplantation/implantation outcomes. This review focuses on recent advances in promoting wound healing and balancing macrophage phenotypes using polysaccharide‐based substrates/coatings and new directions to address the limitations in the current understanding of macrophage responses to polysaccharides.

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“…Ultimately, pure alginate microsphere stimulates M1 polarization with a high level of pro‐inflammatory cytokines secretion. [ 192 ]…”

Section: Influence Of Polysaccharide‐coated Particles On Macrophagementioning

confidence: 99%

The Influence of Polysaccharides‐Based Material on Macrophage Phenotypes

Bratlie

2021

Macromolecular Bioscience

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“…The use of biopolymers in mucosally-administered vaccines has substantially improved the current vaccine formulations and has great potential for the future (204). Some of the presently investigated biopolymers include alginate (205)(206)(207)(208)(209)(210)(211)(212) and gellan (213,214). Although these are still in early stages of study, other biopolymers, such as chitosan (95-97, 215-232), starch (233), and β-glucan (234-241), have already been tested in animal trials with encouraging success.…”

Section: Mucosal Adjuvantsmentioning

confidence: 99%

Immunomodulation as a Novel Strategy for Prevention and Treatment of Bordetella spp. Infections

2019

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Well-adapted pathogens have evolved to survive the many challenges of a robust immune response. Defending against all host antimicrobials simultaneously would be exceedingly difficult, if not impossible, so many co-evolved organisms utilize immunomodulatory tools to subvert, distract, and/or evade the host immune response. Bordetella spp. present many examples of the diversity of immunomodulators and an exceptional experimental system in which to study them. Recent advances in this experimental system suggest strategies for interventions that tweak immunity to disrupt bacterial immunomodulation, engaging more effective host immunity to better prevent and treat infections. Here we review advances in the understanding of respiratory pathogens, with special focus on Bordetella spp., and prospects for the use of immune-stimulatory interventions in the prevention and treatment of infection.

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“…Several alginate-based particles with controllable drug encapsulation efficiencies and release profiles have been reported [ 38 , 39 ]. These particles preserve the bioactivity of small molecules and various drugs, including proteins [ 40 , 41 ] and cytokines [ 42 , 43 ]. Their hydrogel network is commonly formed via ionic cross-linking of alginate with divalent cations [ 38 , 44 , 45 , 46 ], such as Ca 2+ and Ba 2+ .…”

Section: Introductionmentioning

confidence: 99%

Anisotropic, Hydrogel Microparticles as pH-Responsive Drug Carriers for Oral Administration of 5-FU

Teora¹,

Panavaité²,

Sun³

et al. 2023

Pharmaceutics

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In the last 20 years, the development of stimuli-responsive drug delivery systems (DDS) has received great attention. Hydrogel microparticles represent one of the candidates with the most potential. However, if the role of the cross-linking method, polymer composition, and concentration on their performance as DDS has been well-studied, still, a lot needs to be explained regarding the effect caused by the morphology. To investigate this, herein, we report the fabrication of PEGDA–ALMA-based microgels with spherical and asymmetric shapes for 5-fluorouracil (5-FU) on-demand loading and in vitro pH-triggered release. Due to anisotropic properties, the asymmetric particles showed an increased drug adsorption and higher pH responsiveness, which in turn led to a higher desorption efficacy at the target pH environment, making them an ideal candidate for oral administration of 5-FU in colorectal cancer. The cytotoxicity of empty spherical microgels was higher than the cytotoxicity of empty asymmetric microgels, suggesting that the gel network’s mechanical proprieties of anisotropic particles were a better three-dimensional environment for the vital functions of cells. Upon treatment with drug-loaded microgels, the HeLa cells’ viability was lower after incubation with asymmetric particles, confirming a minor release of 5-FU from spherical particles.

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Alginate Microspheres Elicit Innate M1-Inflammatory Response in Macrophages Leading to Bacillary Killing

Cited by 11 publications

References 41 publications

The Influence of Polysaccharides‐Based Material on Macrophage Phenotypes

The Influence of Polysaccharides‐Based Material on Macrophage Phenotypes

Immunomodulation as a Novel Strategy for Prevention and Treatment of Bordetella spp. Infections

Anisotropic, Hydrogel Microparticles as pH-Responsive Drug Carriers for Oral Administration of 5-FU

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