Alginate/poloxamer hydrogel obtained by thiol-acrylate photopolymerization for the alleviation of the inflammatory response of human keratinocytes

Popescu, Irina; Turtoi, Mihaela; Suflet, Dana Mihaela; Dinu, Maria Valentina; Darie-Niță, Raluca Nicoleta; Anghelache, Maria; Calin, Manuela; Constantin, Marieta

doi:10.1016/j.ijbiomac.2021.03.082

Cited by 41 publications

(29 citation statements)

References 61 publications

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“…The separated proteins were transferred (using a Trans-Blot Semi-Dry transfer cell, Bio-Rad) to a nitrocellulose membrane (0.45 µm, Bio-Rad), blocked with 1% BSA for 1 hour [ 29 ], and incubated overnight with the primary antibody: Mouse anti-insulin receptor-β chain (INS R-β) (1:500, Santa Cruz Biotechnology cat. No.…”

Section: Methodsmentioning

confidence: 99%

Synthesis, Characterization, and In Vitro Insulin-Mimetic Activity Evaluation of Valine Schiff Base Coordination Compounds of Oxidovanadium(V)

et al. 2021

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Type 2 diabetes became an alarming global health issue since the existing drugs do not prevent its progression. Herein, we aimed to synthesize and characterize a family of oxidovanadium(V) complexes with Schiff base ligands derived from L-/D-valine (val) and salicylaldehyde (sal) or o-vanillin (van) as insulin-mimetic agents and to assess their potential anti-diabetic properties. Two new oxidovanadium(V) complexes, [{VVO(R-salval)(H2O)}(μ2-O){VVO(R-salval)}] and [{VVO(R-vanval)(CH3OH)}2(μ2-O)], and their S-enantiomers were synthesized and characterized. The compounds exhibit optical activity as shown by crystallographic and spectroscopic data. The stability, the capacity to bind bovine serum albumin (BSA), the cytotoxicity against human hepatoma cell line, as well as the potential anti-diabetic activity of the four compounds are investigated. The synthesized compounds are stable for up to three hours in physiological conditions and exhibit a high capacity of binding to BSA. Furthermore, the synthesized compounds display cytocompatibility at biologically relevant concentrations, exert anti-diabetic potential and insulin-mimetic activities by inhibiting the α-amylase and protein tyrosine phosphatase activity, and a long-term increase of insulin receptor phosphorylation compared to the insulin hormone. Thus, the in vitro anti-diabetic potential and insulin-mimetic properties of the newly synthesized oxidovanadium(V) compounds, correlated with their cytocompatibility, make them promising candidates for further investigation as anti-diabetic drugs.

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Section: Methodsmentioning

confidence: 99%

Synthesis, Characterization, and In Vitro Insulin-Mimetic Activity Evaluation of Valine Schiff Base Coordination Compounds of Oxidovanadium(V)

et al. 2021

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“…To evaluate the anti-inflammatory potential of MNP-Dex/PCA, M1 polarized macrophages were incubated for 48 h with MNP-Dex/PCA containing 350 µM PCA, and relevant controls (plain MNP-Dex, free PCA, and Dexa). Then, the IL-6 cytokine level was measured in culture medium using a bead-based flow cytometry technique (Human IL-6 Flex Set Kit, BD Biosciences, San Jose, CA, USA) as previously described [ 29 ]. Samples were centrifuged at 10,000 rpm for 10 min and analyzed using the Gallios TM flow cytometer (Beckman Coulter Life Sciences, Brea, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Development of Dextran-Coated Magnetic Nanoparticles Loaded with Protocatechuic Acid for Vascular Inflammation Therapy

Anghelache

Turtoi

Petrovici³

et al. 2021

Pharmaceutics

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Vascular inflammation plays a crucial role in the progression of various pathologies, including atherosclerosis (AS), and thus it has become an attractive therapeutic target. The protocatechuic acid (PCA), one of the main metabolites of complex polyphenols, is endowed with anti-inflammatory activity, but its formulation into nanocarriers may increase its bioavailability. In this study, we developed and characterized dextran shell‒iron oxide core nanoparticles loaded with PCA (MNP-Dex/PCA) and assessed their cytotoxicity and anti-inflammatory potential on cells acting as key players in the onset and progression of AS, namely, endothelial cells (EC) and monocytes/macrophages. The results showed that MNP-Dex/PCA exert an anti-inflammatory activity at non-cytotoxic and therapeutically relevant concentrations of PCA (350 μM) as supported by the reduced levels of inflammatory molecules such as MCP-1, IL-1β, TNF-α, IL-6, and CCR2 in activated EC and M1-type macrophages and functional monocyte adhesion assay. The anti-inflammatory effect of MNP-Dex/PCA was associated with the reduction in the levels of ERK1/2 and p38-α mitogen-activated protein kinases (MAPKs) and NF-kB transcription factor. Our data support the further development of dextran shell-magnetic core nanoparticles as theranostic nanoparticles for guidance, imaging, and therapy of vascular inflammation using PCA or other anti-inflammatory compounds.

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“…For the coupling of methacrylate/acrylate, methacryloyl chloride/acryloyl chloride reacts with hydroxyl groups on both ends, resulting in higher mechanical properties [241,242]. In this context, in 2021, Popescu et al developed a hydrogel from a natural polymer and poloxamer 407 obtained by thiol-acrylate photopolymerization to be employed as a wound dressing [243].…”

Section: Derivativesmentioning

confidence: 99%

Non-Ionic Surfactants for Stabilization of Polymeric Nanoparticles for Biomedical Uses

et al. 2021

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Surfactants are essential in the manufacture of polymeric nanoparticles by emulsion formation methods and to preserve the stability of carriers in liquid media. The deposition of non-ionic surfactants at the interface allows a considerable reduction of the globule of the emulsion with high biocompatibility and the possibility of oscillating the final sizes in a wide nanometric range. Therefore, this review presents an analysis of the three principal non-ionic surfactants utilized in the manufacture of polymeric nanoparticles; polysorbates, poly(vinyl alcohol), and poloxamers. We included a section on general properties and uses and a comprehensive compilation of formulations with each principal non-ionic surfactant. Then, we highlight a section on the interaction of non-ionic surfactants with biological barriers to emphasize that the function of surfactants is not limited to stabilizing the dispersion of nanoparticles and has a broad impact on pharmacokinetics. Finally, the last section corresponds to a recommendation in the experimental approach for choosing a surfactant applying the systematic methodology of Quality by Design.

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Alginate/poloxamer hydrogel obtained by thiol-acrylate photopolymerization for the alleviation of the inflammatory response of human keratinocytes

Cited by 41 publications

References 61 publications

Synthesis, Characterization, and In Vitro Insulin-Mimetic Activity Evaluation of Valine Schiff Base Coordination Compounds of Oxidovanadium(V)

Synthesis, Characterization, and In Vitro Insulin-Mimetic Activity Evaluation of Valine Schiff Base Coordination Compounds of Oxidovanadium(V)

Development of Dextran-Coated Magnetic Nanoparticles Loaded with Protocatechuic Acid for Vascular Inflammation Therapy

Non-Ionic Surfactants for Stabilization of Polymeric Nanoparticles for Biomedical Uses

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