Alginate self-adhesive hydrogel combined with dental pulp stem cells and FGF21 repairs hemisection spinal cord injury via apoptosis and autophagy mechanisms

Zhu, Sipin; Ying, Yibo; Wu, Qiuji; Ni, Zhichao; Huang, Zhiyang; Cai, Peihan; Tu, Yurong; Ying, Weiyang; Ye, Jiahui; Zhang, Renkan; Zhang, Yifan; Chen, Min; Xiang, Ziyue; Dou, Haicheng; Huang, Qi-Shan; Li, Xiaokun; He, Huacheng; Xiao, Jian; Ye, Qingsong; Wang, Zhouguang

doi:10.1016/j.cej.2021.130827

Cited by 29 publications

(24 citation statements)

References 79 publications

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“…Most previous studies focused on the development of differentiation medium to induce MSCs into cells derived from three different germ layers ( Lee et al, 2004 ; Xu et al, 2004 ; Anghileri et al, 2008 ; Pavlova et al, 2012 ; Andrzejewska et al, 2019 ; Higuchi et al, 2019 ), and these studies demonstrated that MSCs had great potential to differentiate into cells derived from all three germ layers ( Lee et al, 2004 ; Xu et al, 2004 ; Anghileri et al, 2008 ; Pavlova et al, 2012 ; Andrzejewska et al, 2019 ; Higuchi et al, 2019 ); in some ways, these characteristics are comparable to hPSCs ( Higuchi et al, 2015 ; Andrzejewska et al, 2019 ). Currently, only a few studies have investigated the effect of cell culture biomaterials on MSC differentiation into specific lineages of cells, especially neural cell lineages ( Prabhakaran et al, 2009 ; Zhang et al, 2016 ; Ghorbani et al, 2018 ; Luo et al, 2018a ; Luo et al, 2018b ; Hsiao et al, 2020 ; Luo et al, 2020 ; Albashari et al, 2021 ; Zhu et al, 2021b ).…”

Section: Introductionmentioning

confidence: 99%

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Gao

Tian

Liu

et al. 2022

Front. Cell Dev. Biol.

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Stem cells serve as an ideal source of tissue regeneration therapy because of their high stemness properties and regenerative activities. Mesenchymal stem cells (MSCs) are considered an excellent source of stem cell therapy because MSCs can be easily obtained without ethical concern and can differentiate into most types of cells in the human body. We prepared cell culture materials combined with synthetic polymeric materials of poly-N-isopropylacrylamide-co-butyl acrylate (PN) and extracellular matrix proteins to investigate the effect of cell culture biomaterials on the differentiation of dental pulp stem cells (DPSCs) into neuronal cells. The DPSCs cultured on poly-L-ornithine (PLO)-coated (TPS-PLO) plates and PLO and PN-coated (TPS-PLO-PN) plates showed excellent neuronal marker (βIII-tubulin and nestin) expression and the highest expansion rate among the culture plates investigated in this study. This result suggests that the TPS-PLO and TPS-PN-PLO plates maintained stable DPSCs proliferation and had good capabilities of differentiating into neuronal cells. TPS-PLO and TPS-PN-PLO plates may have high potentials as cell culture biomaterials for the differentiation of MSCs into several neural cells, such as cells in the central nervous system, retinal cells, retinal organoids and oligodendrocytes, which will expand the sources of cells for stem cell therapies in the future.

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Section: Introductionmentioning

confidence: 99%

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Gao

Tian

Liu

et al. 2022

Front. Cell Dev. Biol.

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“…As the methacrylation degree increases, the cross-linking degree increases and the pore size of the freeze-dried hydrogels decreases (Figure 3c,d). Since the coexistence and the distribution of metal ions in the hydrogel can be identified by energy dispersive spectroscopy (EDS), 14 we also conducted EDS to further confirm the homogeneousness of the hydrogels. The characteristic element peaks of the gallium in the EDS spectra indicate that Ga 3+ have been incorporated into both Ga 3+ -cross-linked and dual-cross-linked hydrogels (Figure S1).…”

Section: H Nmr (12)mentioning

confidence: 99%

“…Alginate is a natural polymer derived from algal material with a homopolymeric structure of (1,4)-linked β- d -mannuronate (M block) and α- l -guluronate (G block) and is often used to make hydrogels. − Because of its biocompatibility, low toxicity, relatively low cost, and similarity to the extracellular matrix, it is widely used in wound healing, , drug delivery, , and tissue engineering applications. , The G-block of alginate is electrochemically cross-linked with divalent or polyvalent metal cations to form a gel with an “Eggshell” structure model . While this gentle gel approach is exciting, alginate hydrogels that interact with ions have some disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Gallium(III)-Mediated Dual-Cross-Linked Alginate Hydrogels with Antibacterial Properties for Promoting Infected Wound Healing

Mao

Kong

et al. 2022

ACS Appl. Mater. Interfaces

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The metal gallium has enormous promise in fighting infections by disrupting bacterial iron metabolism via a “Trojan horse” trick. It is well worth trying to study the potential of gallium-mediated hydrogel for treating infected wounds. Herein, on the basis of a conventional gelation strategy of sodium alginate combined with metal ions, Ga3+ has been innovatively given a dual role in a dual-cross-linked hydrogel. It acts nor only as a cross-linking agent to form a hydrogel material but also as a therapeutic agent to slow-release and continuously treat infected wounds. Further photo-cross-linking is introduced to improve the mechanical properties of the hydrogel. Thus, a new gallium ionic- and photo-dual-cross-linked alginate hydrogel, with broad-spectrum antimicrobial activity and strengthened mechanical performance, for the treatment of infected wounds is reported. The morphology, degradability, swelling behavior, rheological properties, and gallium release kinetics together indicated the homogeneous and the strengthened mechanical performance of this hydrogel but did not impede the release of gallium ions. Interestingly, in vitro and in vivo results also demonstrated its favorable biocompatibility, reduced bacterial growth, and accelerated infected wound healing, making the gallium-incorporated hydrogel an ideal antimicrobial dressing.

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“…HGs can also be used to simultaneously release drugs and different growth factors with or without cells embedded in the 3D network [ 172 ]. Following this strategy, the synergistic release of methylprednisolone sodium succinate and growth factors can protect axons and tissues from secondary injury, and promotes scar-boundary- and cavity-free wound healing, resulting in permissive bridges for axonal regrowth [ 2 ].…”

Section: Drug Delivery To the Spinal Cord: The Role Of Biomaterials I...mentioning

confidence: 99%

Biomaterial-Mediated Factor Delivery for Spinal Cord Injury Treatment

et al. 2022

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Spinal cord injury (SCI) is an injurious process that begins with immediate physical damage to the spinal cord and associated tissues during an acute traumatic event. However, the tissue damage expands in both intensity and volume in the subsequent subacute phase. At this stage, numerous events exacerbate the pathological condition, and therein lies the main cause of post-traumatic neural degeneration, which then ends with the chronic phase. In recent years, therapeutic interventions addressing different neurodegenerative mechanisms have been proposed, but have met with limited success when translated into clinical settings. The underlying reasons for this are that the pathogenesis of SCI is a continued multifactorial disease, and the treatment of only one factor is not sufficient to curb neural degeneration and resulting paralysis. Recent advances have led to the development of biomaterials aiming to promote in situ combinatorial strategies using drugs/biomolecules to achieve a maximized multitarget approach. This review provides an overview of single and combinatorial regenerative-factor-based treatments as well as potential delivery options to treat SCIs.

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Alginate self-adhesive hydrogel combined with dental pulp stem cells and FGF21 repairs hemisection spinal cord injury via apoptosis and autophagy mechanisms

Cited by 29 publications

References 79 publications

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Gallium(III)-Mediated Dual-Cross-Linked Alginate Hydrogels with Antibacterial Properties for Promoting Infected Wound Healing

Biomaterial-Mediated Factor Delivery for Spinal Cord Injury Treatment

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