Alginate type and RGD density control myoblast phenotype

Rowley, Jon A.; Mooney, David J.

doi:10.1002/jbm.1287.abs

Cited by 148 publications

(222 citation statements)

References 25 publications

Supporting

Mentioning

198

Contrasting

Unclassified

Order By: Relevance

“…Importantly, it has to be noticed that this detachment was not attributed to a film rupture, as was checked by confocal laser scanning microscopy using fluorescently labeled PLL for visualizing the film [48] (data not shown). Such aggregate formation and spontaneous detachment after 2-3 days has indeed already been observed for C2C12 cells cultured on alginate matrices of various compositions [15] . In this case, the cells that tended to aggregate were apparently the non-fused ones.…”

Section: Effect Of Film Cross-linking On Early Differentiationsupporting

confidence: 72%

“…Most of these studies deal with "model" 3D gels of polyacrylamide (PA) or polydimethylsiloxane (PDMS), which can be prepared easily and whose elasticity can be tuned by varying the cross-linker concentration [6,14] . Biodegradable alginate gels were also used to investigate the relation between chondrocyte and myoblast adhesion and gel elasticity [12,15] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

246

263

View full text Add to dashboard Cite

Beside chemical properties and topographical features, mechanical properties of gels have been recently demonstrated to play an important role in various cellular processes, including cell attachment, proliferation, and differentiation. In this work, we used multilayer films made of poly (L-lysine)/Hyaluronan (PLL/HA) of controlled stiffness to investigate the effects of mechanical properties of thin films on skeletal muscle cells (C2C12 cells) differentiation. Prior to differentiation, cells need to adhere and proliferate in growth medium. Stiff films (E 0 > 320 kPa) promoted formation of focal adhesions and organization of the cytoskeleton as well as an enhanced proliferation, whereas soft films were not favorable for cell anchoring, spreading or proliferation. Then C2C12 cells were switched to a low serum containing medium to induce cell differentiation, which was also greatly dependent on film stiffness. Although myogenin and troponin T expressions were only moderately affected by film stiffness, the morphology of the myotubes exhibited striking stiffness-dependent differences. Soft films allowed differentiation only for few days and the myotubes were very short and thick. Cell clumping followed by aggregates detachment could be observed after ~2 to 4 days. On stiffer films, significantly more elongated and thinner myotubes were observed for up to ~ 2 weeks. Myotube striation was also observed but only for the stiffer films. These results demonstrate that film stiffness modulates deeply adhesion, proliferation and differentiation, each of these processes having its own stiffness requirement.

show abstract

Section: Effect Of Film Cross-linking On Early Differentiationsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

246

263

View full text Add to dashboard Cite

show abstract

“…The density of adhesion ligands, similar to past work with intact ECM molecules, regulates the adhesion, proliferation, and differentiation of cell populations of interest in tissue engineering and regeneration. [48][49][50] However, the nanoscale organization of these ligands appears to modulate the cell response as well, 51 and may be as important as the overall density of ligands presented to cells. Further, the mechanical properties of the synthetic ECM presenting the ligands is equally important, and can significantly alter the response of cells to a specific ligand presentation (type of ligand, density, and nanoscale organization).…”

Section: Cell Instructive Polymers Adhesion Ligand Presentationmentioning

confidence: 99%

Regenerative medicine in orthopaedic surgery

Corsi

Schwarz

Mooney

et al. 2007

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

Regenerative medicine holds great promise for orthopaedic surgery. As surgeons continue to face challenges regarding the healing of diseased or injured musculoskeletal tissues, regenerative medicine aims to develop novel therapies that will replace, repair, or promote tissue regeneration. This review article will provide an overview of the different research areas involved in regenerative medicine, such as stem cells, bioinductive factors, and scaffolds. The potential use of stem cells for orthopaedic tissue engineering will be addressed by presenting the current progress with skeletal muscle-derived stem cells. As well, the development of a revascularized massive allograft will be described and will serve as a prototypic model of orthopaedic tissue engineering. Lastly, we will describe current approaches used to design cell instructive materials and how they can be used to promote and regulate the formation of bony tissue. ß

show abstract

“…16 Because unmodified alginate molecules are highly inert to cell adhesion and protein adsorption, 17 cellular adhesion to gels functionalized with RGD peptides is solely attributed to the bonding between cell receptors and RGD peptides.…”

mentioning

confidence: 99%

Nanoscale Cell Adhesion Ligand Presentation Regulates Nonviral Gene Delivery and Expression

2006

Self Cite

View full text Add to dashboard Cite

It is hypothesized that the nanoscale organization of cell adhesion ligands in a synthetic ECM regulates non-viral gene delivery. This hypothesis was examined with pre-osteoblasts cultured on substrates which present varied density and spacing of synthetic adhesion ligands. The levels of gene transfer and expression were increased with the density of adhesion ligands, but decreased with the spacing of ligands, due to changes in the cell growth rate. This study provides a material-based control point at the nanometer scale for non-viral gene based therapies. Keywords RGD peptides; plasmid DNA; gel matrix; proliferationNon-viral gene vectors [e.g., plasmid DNA (pDNA)] are being increasingly used in a variety of therapeutic applications 1-3 because of safety concerns related to viral vectors. 4 One of the major challenges with the use of non-viral gene vectors is the very limited range of transgene expression level, owing to the poor efficiency of gene transfer. 5 Therefore, there have been extensive efforts to design novel gene carriers to improve the levels of gene transfer and expression using lipids, polycations, dendrimers, nanorods, and oligopeptides 5-7 and efforts to spatially concentrate the pDNA. 8 In contrast, the design of gene delivery systems has largely ignored the importance of the cellular microenvironment in gene uptake and expression, although target tissues for in vivo gene delivery present varying cellular microenvironments.Gene transfer is related to the rate of cell proliferation, 9 and the chemistry and mechanics of the ECM to which a cell adheres regulates many aspects of cell phenotype, including proliferation, apoptosis and differentiation. 10-14 These findings suggest that alteration of the ECM may allow one to regulate a cell's ability to take up and express exogeneous genes. Recently, we have demonstrated that the mechanical rigidity of synthetic ECM used as adhesion substrates plays a critical role in modulating gene transfer and expression. 15 In this study, we demonstrate a new material-based control point to regulate non-viral delivery by engineering the nanoscale organization of cell adhesion molecules in the synthetic ECM. The underlying mechanism of this regulation appears to be related to the material control over cell proliferation. The spatial distribution of cell adhesion molecules at the nanometer scale was controlled using a gel matrix formed from cross-linked alginate molecules containing covalently bound synthetic oligopeptides containing the Arg-Gly-Asp (RGD) sequence (RGD peptides) which mediate cell adhesion. 16 Because unmodified alginate molecules are highly inert to cell adhesion and protein adsorption, 17 cellular adhesion to gels functionalized with RGD peptides is solely attributed to the bonding between cell receptors and RGD peptides.The overall density of RGD peptides (N RGD ) and the distance between islands of RGD peptides (d RGD ), which are known to regulate cell proliferation 11 , were used as variables to control the spatial organization of RGD ...

show abstract

Alginate type and RGD density control myoblast phenotype

Cited by 148 publications

References 25 publications

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Regenerative medicine in orthopaedic surgery

Nanoscale Cell Adhesion Ligand Presentation Regulates Nonviral Gene Delivery and Expression

Contact Info

Product

Resources

About