Alicyclobacillus herbarius sp. nov., a novel bacterium containing omega-cycloheptane fatty acids, isolated from herbal tea.

Abstract

A thermo-acidophilic gram-positive bacterium, strain CP-1T, which grows aerobically at 35-65 degrees C (optimum 55-60 degrees C) and at pH 3.5-6.0 (optimum pH 4.5-5.0), was isolated from a herbal tea made from the dried flowers of hibiscus. Phylogenetic analysis of the 16S rRNA gene sequences showed that this bacterium was clearly distinguishable from previously described species of the genera Alicyclobacillus and Sulfobacillus. Strain CP-1T had unique omega-cycloheptane fatty acids as the major membrane lipid component, a characteristic which is peculiar to Alicyclobacillus cycloheptanicus. However, phenotypic and chemotaxonomic characteristics of strain CP-1T were different from those of the type strain of A. cycloheptanicus. DNA-DNA hybridization between the type strains of Alicyclobacillus species and Sulfobacillus disulfidooxidans was <20%, indicating that strain CP-1T represents a distinct species. On the basis of these results, the name Alicyclobacillus herbarius is proposed for this organism. The type strain is strain CP-1T (= DSM 13609T = IAM 14883T = NRIC 0477T).

Polyamine analysis for chemotaxonomy of thermophilic eubacteria: Polyamine distribution profiles within the orders Aquificales, Thermotogales, Thermodesulfobacteriales, Thermales, Thermoanaerobacteriales, Clostridiales and Bacillales

“…The family Alicyclobacillaceae of the order Bacillales includes mesophilic, acidophiles and moderately (or slightly) thermophilic acidophiles (Dufresne et al, 1996;Goto et al, 2002aGoto et al, , b, 2003Matsubara et al, 2002;Tsuruoka et al, 2003). When two Sulfobacillus species were cultivated at optimum growth temperature, spermine level was higher at higher temperatures.…”

“…The thermophilic anaerobes, Gelria (Plugge et al, 2002), Thermanaerovibrio (Baena et al, 1999b), Thermosyntropha (Svetlitshnyi et al, 1996), Thermicanus (Gossner et al, 1999) and Thermobrachium (Engle et al, 1996), and the mesophiles, Aminobacterium and Aminomonas (Baena et al, 1999a(Baena et al, , 2000, and several anaerobes located in the Desulfotomaculum lineage I (Brauman et al, 1998;Fardeau et al, 1995;Imachi et al, 2002;Qiu et al, 2003;Robertson et al, 2001), and the alkaliphilic clostridia, Alkaliphilus (Cao et al, 2003;Takai et al, 2001b) were available in the present study. Polyamines of thermophilic Anoxybacillus and Ureibacillus (Belduz et al, 2003;Fortina et al, 2001;Yumoto et al, 2004), as well as thermoacidophilic Sulfobacillus and Alicyclobacillus (Dufresne et al, 1996;Goto et al, 2002aGoto et al, , b, 2003Matsubara et al, 2002;Tsuruoka et al, 2003), were also determined.…”

Use of Phospholipid Fatty Acids To Detect Previous Self-Heating Events in Stored Peat

“…5B) and could probably compete well with other organisms, since T1 became a dominant PLFA after prolonged incubations at 55°C. The identification of T1 as an -cycloheptyl and T2 and T3 as -cyclohexyl fatty acids indicated that the microorganisms were members of the genus Alicyclobacillus, which is characterized by large amounts of these rare fatty acids (4,10,19). Among Bacillus-related genera, Alicyclobacillus is characterized as thermophilic, aerobic, heterotrophic, and preferring acid conditions (4,5), which fits with the environmental conditions in heated peat.…”

Alicyclobacillus acidiphilus sp. nov., a novel thermo-acidophilic, omega-alicyclic fatty acid-containing bacterium isolated from acidic beverages.

“…Recently, Alicyclobacillus hesperidum (Albuquerque et al, 2000), ' Alicyclobacillus mali ' and ' Alicyclobacillus acidocaldarius subsp. rittmannii ' (Nicolaus et al, 1998), which possess ω-cyclohexane fatty acid, and Alicyclobacillus herbarius (Goto et al, 2002), which possesses ω-cycloheptane fatty acid, have been reported. In the preceding studies, strains of the genus Alicyclobacillus had been isolated from geothermal sources, and, subsequently, also from non-geothermal soils.…”