Alien introgressions and chromosomal rearrangements do not affect the activity of gliadin-coding genes in hybrid lines of Triticum aestivum L. × Aegilops columnaris Zhuk

Chromosome and Molecular Analyses Reveal Significant Karyotype Diversity and Provide New Evidence on the Origin of Aegilops columnaris

“…The spectra of wheat cultivar Bezostaya−1 (a standard for gliadin spectra of common wheat) and Ae. columnaris K−1193 with the known genetic control of gliadin components [ 27 ] were used to compare gliadin profiles of other Aegilops accessions ( Figure 9 a).…”

“…The spectra of wheat cultivar Bezostaya−1 (a standard for gliadin spectra of common wheat) and Ae. columnaris K−1193 with the known genetic control of gliadin components [27] were used to compare gliadin profiles of other Aegilops accessions (Figure 9a). Amplification of the U-genome-specific U31 nuclear fragment was performed using primers U31a and U31b [23] with PCR conditions: an initial denaturation step of 95 • C for 5 min followed by 30 cycles of 94 • C for 1 min, 55 • C for 1 min, and 72 • C for 1 min with a final extension step at 72 • C for 3 min.…”

“…Blocks of linked electrophoretic gliadin components controlled by a single locus of the particular Ae. columnaris chromosome [ 27 ] are shown schematically at the right side of the electrophoretic spectrum; ( b ) EP spectrum of the accession TA2084 in comparison with wheat cultivar Bezostaya−1; ( c ) EP spectrum of PI 564180; ( d ) EP spectra of Ae. columnaris accessions illustrating protein components presumably encoded by the X c (red dots) and U c (yellow dots) chromosomes.…”

“…These introgression lines also enabled the identification of gliadin components encoded by particular Ae. columnaris chromosomes [ 27 ]. Although extensive polymorphism of electrophoretic spectra of gliadins was demonstrated for durum and common wheat [ 28 ], these markers have been broadly exploited for wheat cultivar identification [ 29 ] diversity of gliadin profiles of Aegilops species, including Ae.…”

Intraspecific divergence of diploid grass Aegilops comosa is associated with structural chromosome changes

“…Since no information on the composition and inheritance of the blocks of gliadin components in this species was available from literature, electrophoretic spectra of all accessions must be compared with an etalon sample with the known genetic control of components. In wheat and related species the gliadin spectrum of bread wheat cultivar Bezostaya-1 serves as an etalon (Novoselskaya-Dragovich et al 2018).…”