Aligned laminin core-polydioxanone/collagen shell fiber matrices effective for neuritogenesis

Abstract

Neural tissue regeneration is a significant challenge, because severe nerve injury is quite difficult to regenerate spontaneously. Although, many studies have been devoted to promote nerve regeneration, there are still many technical challenges to achieve satisfactory results. In this study, we designed biomimetic matrices composed of aligned laminin core-polydioxanone/collagen shell (Lam-PDO/Col) fibers, which can provide both topographical and biochemical cues for promoting neuritogenesis. The aligned Lam-PDO/Col core-shell fiber matrices were fabricated by magnetic field-assisted electrospinning with the coaxial system, and their potential as biofunctional scaffolds for promoting neuritogenesis was explored. It was demonstrated that the aligned Lam-PDO/Col core-shell fibers were successfully fabricated, and the laminin in the core of fibers was steadily and continuously released from fibers. In addition, the cellular behaviors of hippocampal neuronal cells on the matrices were significantly enhanced. Moreover, the aligned Lam-PDO/Col fiber matrices effectively improved and guided neurite outgrowth as well as the neurogenic differentiation by providing both topographical and biochemical cues through aligned fiber structure and sustained release of laminin. Collectively, it is suggested that the aligned Lam-PDO/Col core-shell fiber matrices are one of the most promising approaches for promoting neuritogenesis and neural tissue regeneration.

Fabrication of tri-layered electrospun polycaprolactone mats with improved sustained drug release profile

“…In vitro cytotoxic study revealed that all groups in the study were not cytotoxic, and the cells grew as layers on the mat for 5 days as shown by SEM images. Notably, the reported tri-layered mats with highly sustained drug release profile are easy and straight forward to fabricate when compared to other methods such as coaxial / triaxial / microfluidic methods 3,17,[19][20][21][55][56][57][58] , hence can serve as a potential drug delivery vehicle in clinical applications. In summary, 12% PCLSW-2 with controlled initial burst and sustained release for 62 days, compared to all other groups in evaluation, seems to be best suited for delivery of IBU.…”

“…Various studies have reported the merits of coaxial/tri-axial fabrication of nanofibrous for sustained release of drugs 3,[19][20][21] . Although such methods yield efficient nanofiberous systems for controlled drug release, the complexity of fabrication process is a drawback.…”

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

“…They showed that such biomaterials promoted neural differentiation of mouse embryonic stem cells (ES-D3 cell line). Song et al [258] produced electrospun polydioxanone/collagen (PDO/Col) and laminin/polydioxanone/collagen (Lam-PDO/Col) core-shell matrices. They demonstrated that Lam-PDO/Col biomaterial possessed interconnected porous structure and moderate mechanical properties (E close to 2.5 MPa).…”

Tunable zero-order drug delivery systems created by modified triaxial electrospinning

“…Even when using an insoluble carrier to achieve sustained release profiles, because of the presence of a substantial quantity of drug at or near the surface of the composite, an initial burst release and eventual tailoring-off are inevitable. Very significant efforts have been made to overcome these issues, including through the fabrication of a range of electrospun nanofibers [63,64]. In the core-shell nanohybrids prepared by modified triaxial electrospinning (F2, F3, F4), a blank CA coating was applied to yield a product with a heterogeneous distribution of drug (Fig.…”