Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers

Maliepaard, Chris; Alston, F. H.; Arkel, Gert van; Brown, Linda Morris; Chevreau, Elisabeth; Dunemann, Frank; Evans, Kate; Gardiner, Susan E.; Guilford, Parry; Heusden, A.W. van; Janse, J.; Laurens, François; Lynn, James R.; Manganaris, A. G.; Nijs, A.P.M. den; Periam, N; Rikkerink, Erik H. A.; Roche, P.; Ryder, Carol D.; Sansavini, S.; Schmidt, Hanna; Tartarini, Stefano; Verhaegh, J. J.; Ginkel, M. Vrielink-van; King, Graham J.

doi:10.1007/s001220050867

Cited by 378 publications

(315 citation statements)

References 42 publications

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“…The large number of duplicated restriction fragment length polymorphism (RFLP) markers that have been found in the PM · FS linkage map (Maliepaard et al 1998) support this hypothesis. In our updated map, the lower part of linkage groups 4 and 12 share two common RFLP markers, MC127 and MC105 (van de Weg,unpublished).…”

Section: Discussionmentioning

confidence: 97%

“…Cultivars used for genomic cloning and DNA isolation Apple (Malus domestica) cultivars Prima (PM) and Fiesta (FS) were used initially for genomic PCR cloning and sequencing because of the availability of a molecular marker linkage map from the progeny of PM · FS (Maliepaard et al 1998). Subsequently, Jonathan (JO) and Discovery (DS) were added because maps from the progenies of JO · PM and FS · DS became accessible (van de Weg et al, unpublished).…”

Section: Methodsmentioning

confidence: 99%

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Linkage map positions and allelic diversity of two Mal d 3 (non-specific lipid transfer protein) genes in the cultivated apple (Malus domestica)

et al. 2005

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Non-specific lipid transfer proteins (nsLTPs) of Rosaceae fruits, such as peach, apricot, cherry, plum and apple, represent major allergens for Mediterranean atopic populations. As a first step in elucidating the genetics of nsLTPs, we directed the research reported here towards identifying the number and location of nsLTP (Mal d 3) genes in the apple genome and determining their allelic diversity. PCR cloning was initially performed on two cultivars, Prima and Fiesta, parents of a core apple mapping progeny in Europe, based on two Mal d 3 sequences (AF221502 and AJ277164) in the GenBank. This resulted in the identification of two distinct sequences (representing two genes) encoding the mature nsLTP proteins. One is identical to accession AF221502 and has been named Mal d 3.01, and the other is new and has been named Mal d 3.02. Subsequent genome walking in the upstream direction and DNA polymorphism analysis revealed that these two genes are intronless and that they could be mapped on two homoeologous segments of linkage groups 12 and 4, respectively. Further cloning and sequencing of the coding and upstream region of both Mal d 3 genes in eight cultivars was performed to identify allelic variation. Assessment of the deduced nsLTP amino acid sequences gave a total of two variants at the protein level for Mal d 3.01 and three for Mal d 3.02. The consequences of our results for allergen nomenclature and the breeding of low allergenic apple cultivars are discussed.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Linkage map positions and allelic diversity of two Mal d 3 (non-specific lipid transfer protein) genes in the cultivated apple (Malus domestica)

et al. 2005

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“…The microsatellite markers used in constructing both genetic maps made it possible to align homologous LGs among the three parents of the two crosses (data not shown). Moreover, additional RFLP markers tend to demonstrate that the apple genome contains many homeologous segments (Maliepaard et al 1998;Van de Weg, unpublished results). Homeologous segments are genomic regions presumed to derive from the same ancestor chromosome, in accordance with the probable allopolyploid origin of the Maloideae subfamily within the Rosaceae (Phipps et al 1991).…”

Section: Discussionmentioning

confidence: 99%

“…Genetic linkage maps of both parents of this progeny have been previously constructed using microsatellite, isozyme, random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLP) markers (Maliepaard et al 1998) and recently completed with additional microsatellite, RFLP and AFLP markers (Van de Weg, unpublished results). A second progeny of 188 individuals was derived from a Fiesta (female) · Discovery (male) (F · D) cross created in 1996 at Plant Research International (Wageningen, The Netherlands).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Several apple genetic linkage maps have already been produced from different segregating F 1 progenies (Hemmat et al 1994;Conner et al 1997;Maliepaard et al 1998;Liebhard et al 2003a), and apple reference linkage maps have been constructed successively from progenies derived from the crosses Prima · Fiesta (Maliepaard et al 1998) and later from Fiesta · Discovery (Liebhard et al 2003a). Major genes and quantitative trait loci (QTLs) for resistance to scab, caused by Venturia inaequalis, and powdery mildew, caused by Podosphaera leucotricha, have been identified, and some have been mapped in apple (Alston et al 2000;Hemmat and Brown 2002;Durel et al 2003;Liebhard et al 2003b;Calenge et al 2004a, b), but no gene for resistance to fire blight has been identified to date.…”

Section: Introductionmentioning

confidence: 99%

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Identification of a major QTL together with several minor additive or epistatic QTLs for resistance to fire blight in apple in two related progenies

Calenge¹,

Drouet²,

Denancé³

et al. 2005

Theor Appl Genet

120

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Although fire blight, caused by the bacterium Erwinia amylovora, is one of the most destructive diseases of apple (Malus · domestica) worldwide, no major, qualitative gene for resistance to this disease has been identified to date in apple. We conducted a quantitative trait locus (QTL) analysis in two F 1 progenies derived from crosses between the cultivars Fiesta and either Discovery or Prima. Both progenies were inoculated in the greenhouse with the same strain of E. amylovora, and the length of necrosis was scored 7 days and 14 days after inoculation. Additive QTLs were identified using the MAPQTL software, and digenic epistatic interactions, which are an indication of putative epistatic QTLs, were detected by two-way analyses of variance. A major QTL explaining 34.3-46.6% of the phenotypic variation was identified on linkage group (LG) 7 of Fiesta in both progenies at the same genetic position. Four minor QTLs were also identified on LGs 3, 12 and 13. In addition, several significant digenic interactions were identified in both progenies. These results confirm the complex polygenic nature of resistance to fire blight in the progenies studied and also reveal the existence of a major QTL on LG7 that is stable in two distinct genetic backgrounds. This QTL could be a valuable target in marker-assisted selection to obtain new, fire blight-resistant apple cultivars and forms a starting point for discovering the function of the genes underlying such QTLs involved in fire blight control.

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Synteny in the Rosaceae

Arús

Yamamoto

Dirlewanger

et al. 2005

Plant Breeding Reviews

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Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers

Cited by 378 publications

References 42 publications

Linkage map positions and allelic diversity of two Mal d 3 (non-specific lipid transfer protein) genes in the cultivated apple (Malus domestica)

Linkage map positions and allelic diversity of two Mal d 3 (non-specific lipid transfer protein) genes in the cultivated apple (Malus domestica)

Identification of a major QTL together with several minor additive or epistatic QTLs for resistance to fire blight in apple in two related progenies

Synteny in the Rosaceae

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