Aliidiomarina halalkaliphila sp. nov., a haloalkaliphilic bacterium isolated from a soda lake in Inner Mongolia Autonomous Region, China

Abstract

A haloalkaliphilic strain (IM 1326T) was isolated from brine sampled at a soda lake in the Inner Mongolia Autonomous Region, China. Cells of the strain were rod-shaped and motile. Strain IM 1326T was able to grow at 4–42 °C (optimum, 37 °C) with 0–13.0 % (w/v) NaCl concentrations (optimum at 4.0–6.0 %) and at pH 7.5–11.0 (optimum at 9.0–10.0). The 16S rRNA gene phylogenetic analysis revealed that the isolate belongs to the genus Aliidiomarina and is closely related to the type strains of Aliidiomarina sanyensis (95.8 % sequence similarity), Aliidiomarina shirensis (95.7 %), Aliidiomarina iranensis (95.4 %) and Aliidiomarina haloalkalitolerans (95.3 %). The whole genome of strain IM 1326T was sequenced, and the genomic DNA G+C content was 49.7 mol%. Average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between the isolate and the related Aliidiomarina species were 68.1–84.9 %, 76–78 % and 18.4–20.4 %, respectively. The respiratory quinone was ubiquinone-8. The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminophospholipid. The predominant cellular fatty acids were summed feature 9 (10-methyl-C16 : 0/iso-C17 : 1 ω9c, 22.2 %), iso-C15 : 0 (16.1 %) and iso-C17 : 0 (13.1 %). Based on the results of phylogenetic analysis, genome relatedness, and the physiological and chemotaxonomic properties of the isolate, strain IM 1326T is considered to represent a novel species of the genus Aliidiomarina , for which the name Aliidiomarina halalkaliphila sp. nov. is proposed (type strain IM 1326T=CGMCC 1.17056T=JCM 34227T).

Aliidiomarina quisquiliarum sp. nov., isolated from landfill leachate

“…The major polar lipids comprised diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), aminoglycophospholipid (APGL), aminophospholipid (APL), phospholipid (PL), three glycolipids (GL1-3) and two unknown lipids (L1 and L2). Strain Y-6 T and A. taiwanensis MCCC 1A06493 T only differed in their contents of GL and L. The predominant respiratory quinone in strain Y-6 T was ubiquinone 8 (Q-8) which was in agreement with all members of the genus Aliidiomarina [1][2][3][4][5][6][7][8][9][10][11]. Compared with A. taiwanensis MCCC 1A06493 T , strain Y-6 T had some differences in the percentage of some fatty acids (Table S1).…”

“…Strain Y-6 T and A. taiwanensis MCCC 1A06493 T only differed in their contents of GL and L. The predominant respiratory quinone in strain Y-6 T was ubiquinone 8 (Q-8) which was in agreement with all members of the genus Aliidiomarina [1–11]. Compared with A.…”

“…The major isoprenoid quinone is ubiquinone 8 (Q-8). The DNA G+C content ranges from 45 to 55 mol% [1][2][3][4][5][6][7][8][9][10][11]. This article describes a strain isolated from a landfill site and which is considered to represent a novel species of the genus Aliidiomarina.…”

“…At the time of writing, the genus comprised twelve recognized species (https://lpsn.dsmz.de/). These species were isolated from various habitats such as seawater, saline lake, saline-alkaline soil, sediment and wetland [1][2][3][4][5][6][7][8][9][10][11]. The members of this family are Gram-stain-negative, mesophilic, aerobic or facultatively anaerobic rods, require sodium ions for growth and show a poor ability to use carbohydrates as sole carbon and energy sources [12].…”