Alkali‐like myosin light chain‐1 (<i>myl1</i>) is an early marker for differentiating fast muscle cells in zebrafish

Burguière, A. C.; Nord, Hanna; Hofsten, Jonas von

doi:10.1002/dvdy.22677

Cited by 30 publications

(24 citation statements)

References 33 publications

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“…This mutation prevents salt bridge formation between residues R281 and D325, thereby destabilizing the myosin head (Budde et al, 2007). Richard et al (2003) reported that changes to the Myh7 genes accounted for 82% of families with identified mutations, emphasizing its relevance for hypertrophic cardiomyopathy The Myl1 gene encodes the fast skeletal alkali myosin light polypeptide 1, and is a specific and early marker for development of fast skeletal muscle (Ling et al, 2010;Burguière et al, 2011). However, several studies also have indicated that Myl1 can be expressed in cardiac muscles including the atria and the left ventricle (Kelly et al, 1995;Franco et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of atrial and ventricular muscles from rats

Zhi

Cao²,

Li³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Previous studies have used microarray technology to explore gene expression differences between the atrium and the ventricle. However, selection criteria for the differentially expressed genes (DEGs) based only on either the fold change or the P value in these studies. Here, we aim to further identify the DEGs by setting a P value threshold of <0.05 and a fold change of >2, which may yield more specific gene expression differences between the atrium and the ventricle. Gene expression profiling of the atrial appendages and the ventricular free walls in 13 normal male Sprague Dawley rats were obtained from the Gene Expression Omnibus data base (accession No.: GSE5266). DEGs between the atrial and the ventricular samples were screened using the microarray significance analysis. The underlying functions of DEGs were predicted by gene ontology and pathway enrichment analyses. In addition, we also constructed protein interactions networks, and analyzed the function modules of the interacting proteins by MCODE. A total of 757DEGs between the atria and the ventricles were found. The genes highly expressed in the ventricular myocytes were associated with muscle contraction (e.g., Myl1, Myl2, Myl3, and Myh7) and energy production (e.g., Acadm and Acsl6), while the genes preferentially expressed in the atrial myocytes were involved in the integration of neurohumoral signals (e.g., Cldn1). These conclusions were confirmed by pathway enrichment and function module analyses. Our present study provides an overview of the transcript level differences between the atrium and the ventricle, which may be useful for determination of potential biomarkers.

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Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of atrial and ventricular muscles from rats

Zhi

Cao²,

Li³

et al. 2016

Genet. Mol. Res.

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“…Skeletal fast specific fibres are formed from the non-adaxial cells in the paraxial mesoderm and genes encoding fast specific components of the sarcomere can be detected in these cells after the 8 somite stage (8 ss) (Burguière et al, 2011). Similarly, expression of six1b can be detected in the same region starting at the 6 ss (Bessarab et al, 2008).…”

Section: Zebrafish Six1 Influences Fast Fibre Formationmentioning

confidence: 99%

Six1 regulates proliferation of Pax7+ muscle progenitors in zebrafish

Nord

Skalman

Hofsten

2013

Journal of Cell Science

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SummaryIn the embryonic zebrafish, skeletal muscle fibres are formed from muscle progenitors in the paraxial mesoderm. The embryonic myotome is mostly constituted of fast-twitch-specific fibres, which are formed from a fast-specific progenitor cell pool. The most lateral fraction of the fast domain in the myotome of zebrafish embryos derives from the Pax7-positive dermomyotome-like cells. In this study, we show that two genes, belonging to the sine oculus class 1 (six1) genes (six1a and six1b), are both essential for the regulation of Pax7 + cell proliferation and, consequently, in their differentiation during the establishment of the zebrafish dermomyotome. In both six1a and six1b morphant embryos, Pax7 + cells are initially formed but fail to proliferate, as detected by reduced levels of the proliferation marker phosphohistone3 and reduced brdU incorporation. In congruence, overexpression of six1a or six1b leads to increased Pax7 + cell number and reduced or alternatively delayed fibre cell differentiation. Bone morphogenetic protein signalling has previously been suggested to inhibit differentiation of Pax7 + cells in the dermomyotome. Here we show that the remaining Pax7 + cells in six1a and six1b morphant embryos also have significantly reduced pSmad1/5/8 levels and propose that this leads to a reduced proliferative activity, which may result in a premature differentiation of Pax7 + cells in the zebrafish dermomyotome. In summary, we show a mechanism for Six1a and Six1b in establishing the Pax7 + cell derived part of the fast muscle and suggest new important roles for Six1 in the regulation of the Pax7 + muscle cell population through pSmad1/5/8 signalling.

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“…Kelly and Buckingham [51] have reported that myl-1 expressed in mouse fast-twitch fibres. Burguie`re and his colleagues [28] had reported myl-1 as earliest expressed marker in the fasttwitch precursor cells in the zebrafish embryo, and its knockdown disrupts myogenesis. In P. latipinna, results revealed down regulation of the myl-1 gene at the wound healing and blastema stage while in the differentiation stage it was found to be increased at both transcript as well as protein level suggesting the involvement of myosin light chain 1/3 during muscle contractility for the movement of the fish, P. latipinna.…”

Section: Discussionmentioning

confidence: 99%

“…As the requirement of keratin to get the structural integrity of newly formed epithelium has been reported in many vertebrates during development, adulthood and regeneration [25,26], further analysis of this peptide at transcript level was attempted. For myosin light chain-1, the gene myl-1, encodes two transcript variants expressed in fast skeletal muscle of zebrafish embryo [27] as well as it was reported in the fast twitch fibres and its knockdown has been shown to disrupt myogenesis [28]. Therefore, it was inferred that myosin light chain-1 is important during later stages of regeneration.…”

Section: Introductionmentioning

confidence: 99%

Temporal Expression Pattern of Peptides in the Regenerating Caudal Fin of Teleost Fish Poecilia latipinna With Special Emphasis on krt15 and myl-1

Murawala¹,

Ranadive²,

Patel³

et al. 2017

Eur J Exp Bio

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Amongst several vertebrates, caudal fin of teleost fish has emerged as an excellent model to understand the mechanism of regeneration. By now it is well perceived that proper coordination of different regulatory signals are needed to facilitate the progression of successive stages of regeneration. Therefore, in the current study protein expression profile for caudal fin regeneration in Poecilia latipnna was brought into the focus. The pattern of protein turnover and variation in the expression level were observed across the stages of regeneration. Data from twodimensional gel electrophoresis revealed the differential expression of proteins for wound epithelium, blastema and differentiation stages when compared to resting stage. Based on the computational analysis, of the peptide expression profile and subsequent sequencing, two peptides namely Keratin type I cytoskeletal 15 and Myosin light chain-1 were studied further at transcript level. Keratin-15 was found to be up regulated at wound epithelium stage while its expression waned significantly in the blastema and differentiation stages of regeneration indicating its role in the formation of a functional wound epithelium. Western blot analysis of Keratin-15 also concurred with transcript levels of krt15. However, myl-1 transcript levels was observed to be dispensable for initial phase of regeneration whereas its elevated level in differentiation stage at both mRNA and protein level marks its noteworthy role in achieving the structural integrity of a regenerating caudal fin. This is the first observation of the involvement of Keratin-15 and Myosin light chain-1 in the regulation of regenerative process in Poecilia latipinna.

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Alkali‐like myosin light chain‐1 (myl1) is an early marker for differentiating fast muscle cells in zebrafish

Cited by 30 publications

References 33 publications

Transcriptional analysis of atrial and ventricular muscles from rats

Transcriptional analysis of atrial and ventricular muscles from rats

Six1 regulates proliferation of Pax7+ muscle progenitors in zebrafish

Temporal Expression Pattern of Peptides in the Regenerating Caudal Fin of Teleost Fish Poecilia latipinna With Special Emphasis on krt15 and myl-1

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