Alkaline organosolv pretreatment of different sorghum stem parts for enhancing the total reducing sugar yields and p-coumaric acid release

Li, Dandan; Long, Liangkun; Ding, Shaojun

doi:10.21203/rs.3.rs-17599/v2

Cited by 1 publication

(1 citation statement)

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“…대표적인 화학적 방법으로는 유기용매 추출 전처 리법 (Organosolv pretreatment) 이 있다. 유기용매 추출 전처 리법은 메탄올, 에탄올과 같은 유기용매에 lignin을 용해시킴 으로써 바이오매스의 구조의 변화를 가져오는 것으로, 다른 물리화학적 방법보다 lignin의 선택적 제거율이 높다고 보고 되었다 [7][8][9][10]. 여기에 산, 알칼리 촉매의 첨가를 통해 lignin 과 hemicellulose의 분해를 촉진시킴으로써 비교적 온화한 조건에서 공정 효율을 향상시킬 수 있다.…”

Section: Introductionunclassified

Development of Alkali-Catalyzed Organosolv Pretreatment and SHF Process for Production of Bio-Succinic Acid from Barley Straw

Kim

Park

Kim

et al. 2022

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A series of biological processes were developed to extract fermentable sugars from barley straw and produce succinic acid (SA), a microbial metabolite. For selectively removal of lignin with the alkali-catalyzed organosolv, process parameters such as NaOH concentration (w/w, %), ethanol concentration (v/v, %) and reaction temperature ( o C) were investigated using the response surface method. As a result, the cellulose content of pretreated materials was increased about 63% and lignin was removed about 80% compared to the raw materials. For obtaining the high amount of SA, separate hydrolysis and fermentation (SHF) process was performed with high amount (113.9 g/L) of pretreated materials in a 5 L bioreactor. Through employing the fed-batch mode with enzyme feeding, the saccharification could be carried out effectively despite the low concentration (5 FPU cellulase/gglucan) of enzyme, resulting in the fermentable sugars of 58.76 g/L and glucose conversion rate of 63.35%. Actinobacillus succinogenes consumed glucose and xylose simultaneously, the main carbon sources of saccharified solution, at the rates of 0.71 and 0.49 g/L/h, respectively, and the phenomenon of catabolite repression was not observed. SA of 32.58 g/ L was produced with a production yield of 0.56 SA g/total sugars g. Finally, it was turned that the A. succinogenes has metabolic capacities that could effectively utilize the carbohydrates of the lignocellulosic biomass, and the saccharified solution derived from barley straw was suitable as fermentation substrate.

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Section: Introductionunclassified