Alkaline phosphatase activity during differentiation of the human promyelocytic leukemia cell line, HL-60

Wei, Lillian L-L.; Hui, Eric Ka-Wai; Wei, Jeng-Shu; Yung, Benjamin Yat‐Ming

doi:10.1016/0006-291x(92)91758-i

Cited by 12 publications

(11 citation statements)

References 9 publications

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“…To avoid individual discrepancies in the determination of the number of cells showing the morphological changes, we have followed the HL60 cell differentiation by the appearance of marker enzymes typical of the differentiated phenotype. Based on a recent report [5] alkaline phosphatase (A1Pase) activity is the marker for the RA-induced differentiation, whereas acid phosphatase (AcPase) is the marker for the PMA-induced differentiation [6], Under our conditions, in the undifferentiated state, the basal level of AIPase is 0.9 + 0.1 unitslmg of membrane proteins; in the fully differentiated cells, the level increases to 75 _+ 0.9 unitslmg of membrane proteins. Similarly, AcPase has a basal level of 17 _+ 2 units/rag membrane proteins in undifferentiated cells and increases to 215 + 12 units/mg of membrane proteins in the fully differentiated cells.…”

Section: Cell Culture and Differentiationmentioning

confidence: 88%

“…We have observed that following the addition of DEF, HL60 cells exposed to suboptimal concentrations of either RA or PMA undergo an acceleration in the rate of differentiation that accounts for a 20-to 40-fold increase over that observed in the presence of the inducer alone. Differentiation was monitored by the increase in the levels of marker enzymes [5,6] and by the appearance of characteristic morphological changes in the cells [6,7]. Furthermore, the results obtained have indicated that, in the early stages of cell differentiation, DEF also induces an increase in the rate and in the extent of variations in the levels of PKC isoforms present in HL60 cells.…”

Section: Discussionmentioning

confidence: 98%

“…These concentrations of inducers are those found to promote the maximal rate of differentiation. Cells were incubated for four days and differentiation was determined by examination of cell morphology under light microscope, following staining with Wright-Giemsa method [5]. Under these conditions approximately 90-92% of cells reached the differentiated state.…”

Section: Cell Culture and Differentiationmentioning

confidence: 99%

“…The amino acid sequence of tryptic peptides, obtained from purified DEF, has indicated no homology with the primary structure of any other natural protein so far characterized [4]. In order to establish if DEF possesses an enhancing effect on the differentiation of other cell types, we have tested the capacity of this protein to affect the differentiation of HL60 promyelocytic leukemia cells, using as a marker the level of specific enzymes, the increase of which had been previously demonstrated to correlate with the differentiation process [5,6]. It has been established that DEF promotes an acceleration in the differentiation of HL60 cells, accompanied by significant changes in the level of PKC isozymes present in these cells.…”

Section: Introductionmentioning

confidence: 99%

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Differentiation of HL60 promyelocytic cells is promoted by a ‘differentiation enhancing factor’ produced by erythroleukemia cells

et al. 1993

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A differentiation enhancing factor isolated from murine erythroleukemia cells is also a potent enhancer of the differentiation of HL60 human promyelocytic leukemia cells, induced by retinoic acid and by phorbol ester. This stimulating effect is the result of a large increase in the sensitivity of HL60 cells for retinoic acid and for phorbol 12-myristate 13-acetate (20-fold and 40-fold, respectively). Accelerated differentiation induced by the protein factor, and monitored by the appearance of marker enzymes, is accompanied by a large increase in the fluctuation of the levels of protein kinase C (PKC) isozymes in HL60 cells. These results provide further support for the role of this new protein factor in cell differentiation and indicate that other cell types are susceptible to its biological effect.

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Section: Cell Culture and Differentiationmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 98%

Section: Cell Culture and Differentiationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differentiation of HL60 promyelocytic cells is promoted by a ‘differentiation enhancing factor’ produced by erythroleukemia cells

et al. 1993

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“…4). In recent years, as widely adopted and used by researchers, the transcription and protein expression of ALP is thought to be a symbol of cellular osteogenic differentiation 31,32) . A higher level of ALP activity indicates an earlier and faster osteogenic differentiation promotion.…”

Section: Discussionmentioning

confidence: 99%

Calcification of MC3T3-E1 cells on titanium and zirconium

et al. 2015

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To confirm similarity of hard tissue compatibility between titanium and zirconium, calcification of MC3T3-E1 cells on titanium and zirconium was evaluated in this study. Mirror-polished titanium (Ti) and zirconium (Zr) disks and zirconium-sputter deposited titanium (Zr/Ti) were employed in this study. The surface of specimens were characterized using scanning electron microscopy and X-ray diffraction. Then, the cellular proliferation, differentiation and calcification of MC3T3-E1 cells on specimens were investigated. The surface of Zr/Ti was much smoother and cleaner than those of Ti and Zr. The proliferation of the cell was the same among three specimens, while the differentiation and calcification on Zr/Ti were faster than those on Ti and Zr. Therefore, Ti and Zr showed the identical hard tissue compatibility according to the evaluation with MC3T3-E1 cells. Sputter deposition may improve cytocompatibility.

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Ecto-alkaline phosphatase activity identified at physiological pH range on intact P19 and HL-60 cells is induced by retinoic acid

et al. 2000

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The activity of membrane-bound alkaline phosphatase (ALP) expressed on the external surface of cultured murine P19 teratocarcinoma and human HL-60 myeloblastic leukemia cells was studied at physiological pH using p-nitrophenylphosphate (pNPP) as substrate. The rate of substrate hydrolysis catalyzed by intact viable cells remained constant for eight successive incubations of 30 min and was optimal at micromolar substrate concentrations over the pH range 7.4-8.5. The value of apparent K(m) for pNPP in P19 and HL-60 cells was 120 microM. Hydrolytic activity of the ecto-enzyme at physiological pH decreased by the addition of levamisole, a specific and noncompetitive inhibitor of ALP (K(i) P19 = 57 microM; K(i) HL-60 = 50 microM). Inhibition of hydrolysis was reversed by removal of levamisole within 30 min. Retinoic acid (RA), which promotes the differentiation of P19 and HL-60 cells, induced levamisole-sensitive ecto-phosphohydrolase activity at pH 7.4. After its autophosphorylation by ecto-kinase activity, a 98-kDa membrane protein in P19 cells was found to be sensitive to ecto-ALP, and protein dephosphorylation increased after incubation of cells with RA for 24 h and 48 h. Orthovanadate, an inhibitor of all phosphatase activities, blocked the levamisole-sensitive dephosphorylation of the membrane phosphoproteins, while (R)-(-)-epinephrine reversed the effect by complexation of the inhibitor. The results demonstrate that the levamisole-sensitive phosphohydrolase activity on the cell surface is consistent with ecto-ALP activity degrading both physiological concentrations of exogenously added substrate and endogenous surface phosphoproteins under physiological pH conditions. The dephosphorylating properties of ecto-ALP are induced by RA, suggesting a specific function in differentiating P19 teratocarcinoma and HL-60 myeloblastic leukemia cells.

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Alkaline phosphatase activity during differentiation of the human promyelocytic leukemia cell line, HL-60

Cited by 12 publications

References 9 publications

Differentiation of HL60 promyelocytic cells is promoted by a ‘differentiation enhancing factor’ produced by erythroleukemia cells

Differentiation of HL60 promyelocytic cells is promoted by a ‘differentiation enhancing factor’ produced by erythroleukemia cells

Calcification of MC3T3-E1 cells on titanium and zirconium

Ecto-alkaline phosphatase activity identified at physiological pH range on intact P19 and HL-60 cells is induced by retinoic acid

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