Alkaline phosphatase can promote the replication of Bombyx mori cypovirus 1 by interaction with its turret protein

Qi, Lu; Ren, Feifei; Yan, Jiming; Zhang, Yinong; Awais, Mian Muhammad; Sun, Jingchen

doi:10.1016/j.virusres.2020.198261

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…Correspondingly, we used RNAi to knockdown the expression of endogenous BmPGRP-S5 in BmN cells, which resulted in the reduced intracellular proliferation of BmCPV1, similar to results obtained in silkworm larvae. In a previous study, we confirmed that alkaline phosphatase (Alp) in midgut tissue promotes the proliferation of BmCPV1 by interacting with the turret protein of BmCPV1 . However, it is unclear whether a functional link exists between Alp and BmPGRP-S5, which needs to be verified in the future.…”

Section: Discussionmentioning

confidence: 76%

“…In previous studies, BmN cells, a commonly used cell line in B. mori research, were considered to be not permissive for BmCPV1 infection while in some research reports transfection was used to infect BmN cells or other B. mori cells with BmCPV1. − In this study, we purified BmCPV1 virions from polyhedra using alkaline lysis followed by ultracentrifugation. Direct addition of the purified BmCPV1 virions to BmN cells resulted in an increase in virus gene expression in the cells, as demonstrated by PCR and western blotting with a specific antibody (anti-VP1), indicating that BmCPV1 virions can infect BmN cells and proliferate within them.…”

Section: Discussionmentioning

confidence: 99%

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Peptidoglycan Recognition Protein S5 of Bombyx mori Facilitates the Proliferation of Bombyx mori Cypovirus 1

Ren

Yan

Wang

et al. 2023

J. Agric. Food Chem.

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Bombyx mori cypovirus 1 (BmCPV1), a primary pathogen of the silkworm, is a typical dsRNA virus belonging to the Reoviridae family. In this study, a total of 2520 differentially expressed genes (DEGs) were identified by RNA-seq analysis of the silkworm midgut after BmCPV1 infection and Gene Ontology (GO) functional annotation showed that the DEGs predominantly functioned in binding (molecular function), cell (cellular component), and cellular processes (biological process). Additionally, the Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation revealed that the DEGs were mainly distributed in global and overview metabolism maps, translation, and signal transduction. Among the identified DEGs, BmPGRP-S5 belongs to the peptidoglycan recognition protein (PGRP) family. Previous studies have revealed that PGRPs were involved in the interactions between silkworm and BmCPV1. Here, we explored the effect of BmPGRP-S5 on BmCPV1 replication and demonstrated that BmPGRP-S5 promotes the proliferation of BmCPV1 in BmN cells through overexpression or knockdown experiments. Knocking down of BmPGRP-S5 in silkworm larvae similarly promoted the proliferation of BmCPV1. Through experimental validation, we therefore determined that BmPGRP-S5 acts as a proviral host factor for BmCPV1 infection. This study clarifies the proliferation mechanism of BmCPV1 and provides new insights into the functional role of BmPGRP-S5.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Peptidoglycan Recognition Protein S5 of Bombyx mori Facilitates the Proliferation of Bombyx mori Cypovirus 1

Ren

Yan

Wang

et al. 2023

J. Agric. Food Chem.

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“…Cypovirus is a member of the Reoviridae family, and is characterized by its single layered capsid [ 4 ]. DnCPV-23 was isolated from naturally diseased D. nerii larvae.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional responses of Daphnis nerii larval midgut to oral infection by Daphnis nerii cypovirus-23

Kuang

Yan

Zhan

et al. 2021

Virol J

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Background Daphnis nerii cypovirus-23 (DnCPV-23) is a new type of cypovirus and has a lethal effect on the oleander hawk moth, Daphnis nerii which feeds on leave of Oleander and Catharanthus et al. After DnCPV-23 infection, the change of Daphnis nerii responses has not been reported. Methods To better understand the pathogenic mechanism of DnCPV-23 infection, 3rd-instar Daphnis nerii larvae were orally infected with DnCPV-23 occlusion bodies and the transcriptional responses of the Daphnis nerii midgut were analyzed 72 h post-infection using RNA-seq. Results The results showed that 1979 differentially expressed Daphnis nerii transcripts in the infected midgut had been identified. KEGG analysis showed that protein digestion and absorption, Toll and Imd signaling pathway were down-regulated. Based on the result, we speculated that food digestion and absorption in insect midgut might be impaired after virus infection. In addition, the down-regulation of the immune response may make D. nerii more susceptible to bacterial infections. Glycerophospholipid metabolism and xenobiotics metabolism were up-regulated. These two types of pathways may affect the viral replication and xenobiotic detoxification of insect, respectively. Conclusion These results may facilitate a better understanding of the changes in Daphnis nerii metabolism during cypovirus infection and serve as a basis for future research on the molecular mechanism of DnCPV-23 invasion.

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Transcriptional Responses of Daphnis nerii Larval Midgut to Oral Infection by 2 Daphnis nerii cypovirus-23

Kuang

Cheng-hua

Zhan

et al. 2021

Preprint

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Background: Daphnis nerii cypovirus-23 (DnCPV-23) is a new type of cypovirus and has lethal effect on the oleander hawk moth, Daphnis nerii , which feeds on leave of Oleander and Catharanthus et al. After DnCPV-23 infection, the change of Daphnis nerii responses has not been reported.Methods: In order to better understand the pathogenic mechanism of DnCPV-23 infection, 3rdinstar Daphnis nerii larvae were orally infected with DnCPV-23 occlusion bodies and the transcriptional responses of the Daphnis nerii midgut were analyzed 72 h post-infection using RNA-seq.Results: The results showed that 1,979 differentially expressed Daphnis nerii transcripts in the infected midgut had been identified. KEGG analysis showed that protein digestion and absorption, Toll and Imd signaling pathway were down-regulated. Based on the result, it was possible the function of food digestion and absorption in insect midgut was impaired after virus infection. In addition, the down-regulation of the immune response may be conducive to viral immune escape. Glycerophospholipid metabolism and xenobiotics metabolism were up-regulated. These two types of pathways may affect the viral replication and xenobiotic detoxification of insect, respectively. Conclusion: These results may facilitate a better understanding of the changes in Daphnis nerii metabolism during cypovirus infection and serve as a basis for future research on the molecular mechanism of DnCPV-23 invasion.

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Alkaline phosphatase can promote the replication of Bombyx mori cypovirus 1 by interaction with its turret protein

Cited by 3 publications

References 22 publications

Peptidoglycan Recognition Protein S5 of Bombyx mori Facilitates the Proliferation of Bombyx mori Cypovirus 1

Peptidoglycan Recognition Protein S5 of Bombyx mori Facilitates the Proliferation of Bombyx mori Cypovirus 1

Transcriptional responses of Daphnis nerii larval midgut to oral infection by Daphnis nerii cypovirus-23

Transcriptional Responses of Daphnis nerii Larval Midgut to Oral Infection by 2 Daphnis nerii cypovirus-23

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