Alkaline Phosphatase-Catalyzed Silver Deposition for Electrochemical Detection

Fanjul‐Bolado, Pablo; Hernández‐Santos, David; González‐García, María Begoña; Costa-Garcı́a, Agustı́n

doi:10.1021/ac070624o

Cited by 109 publications

(76 citation statements)

References 33 publications

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“…The sensor was then rinsed with Tris-HNO 3 (pH 9.8) buffer, and the enzymatic reaction was carried out by placing 40 μl of a 3-IP (1.0×10 −3 M) and silver nitrate (4.0× 10 −4 M) solution on the immunosensor's surface. After 20 min, a linear sweep voltammogram was recorded from −0.02 to +0.4 V, at a scan rate of 50 mV/s, to obtain the electrochemical current of the enzymatically deposited silver [25]. Figure 2 depicts a schematic representation of this immunoassay.…”

Section: Immunosensor Assaymentioning

confidence: 99%

Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

et al. 2015

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A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using twomonoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.

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Section: Immunosensor Assaymentioning

confidence: 99%

Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

et al. 2015

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“…2) consisted of the following steps: (i) incubation with the antigen (HER2 ECD) (40 mL; 1 h); (ii) addition of 40 mL of a biotinylated detection antibody solution (1 h); (iii) addition of 40 mL of an S-AP solution (1 h); (iv) addition of 40 mL of a solution containing the enzymatic substrate (3-IP) (1.0 mM) and silver ions (0.40 mM) (20 min) (v) detection of the analytical signal by linear sweep voltammetry (potential scan: À 0.03 to þ 0.4 V; scan rate: 0.05 V/s). This signal was obtained through the anodic stripping of the enzymatically generated metallic silver [17]. After steps (i) and (ii) the SPCE was washed with Buffer 1 and dried.…”

Section: Electrochemical Immunoassaymentioning

confidence: 99%

Electrochemical immunosensor for the analysis of the breast cancer biomarker HER2 ECD

Marques

Subramanian

Nouws

et al. 2014

Talanta

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a b s t r a c tHuman epidermal growth factor receptor 2 (HER2) is a breast cancer biomarker that plays a major role in promoting breast cancer cell proliferation and malignant growth. The extracellular domain (ECD) of HER2 can be shed into the blood stream and its concentration is measurable in the serum fraction of blood. In this work an electrochemical immunosensor for the analysis of HER2 ECD in human serum samples was developed. To achieve this goal a screen-printed carbon electrode, modified with gold nanoparticles, was used as transducer surface. A sandwich immunoassay, using two monoclonal antibodies, was employed and the detection of the antibody-antigen interaction was performed through the analysis of an enzymatic reaction product by linear sweep voltammetry. Using the optimized experimental conditions the calibration curve (i p vs. log[HER2 ECD]) was established between 15 and 100 ng/mL and a limit of detection (LOD) of 4.4 ng/mL was achieved. These results indicate that the developed immunosensor could be a promising tool in breast cancer diagnostics, patient follow-up and monitoring of metastatic breast cancer since it allows quantification in a useful concentration range and has an LOD below the established cut-off value (15 ng/mL).

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“…The immunosensing strategy was based on a sandwich format in which two monoclonal mouse IgG antibodies against Ara h 1 were used as capture and detection antibodies. The detection antibody used in this work was labelled with alkaline phosphatase and the electrochemical detection relied on an enzyme-catalyzed metal precipitation followed by applying an anodic (stripping) voltammetric potential scan (Fanjul-Bolado et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Detection of Ara h 1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensor

Alves

Pimentel

Nouws

et al. 2015

Biosensors and Bioelectronics

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Keywords:Electrochemical immunosensor Biosensor Screen-printed carbon electrode Allergen Peanut Ara h 1 a b s t r a c t A gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in the development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection in food samples. Gold nanoparticles (average diameter¼32 nm) were electrochemically generated on the surface of screen-printed carbon electrodes. Two monoclonal antibodies were used in a sandwich-type immunoassay and the antibody-antigen interaction was electrochemically detected through stripping analysis of enzymatically (using alkaline phosphatase) deposited silver. The total time of the optimized immunoassay was 3 h 50 min. The developed immunosensor allowed the quantification of Ara h 1 between 12.6 and 2000 ng/ml, with a limit of detection of 3.8 ng/ml, and provided precise (RSD o8.7%) and accurate (recovery 496.6%) results. The immunosensor was successfully applied to the analysis of complex food matrices (cookies and chocolate), being able to detect Ara h 1 in samples containing 0.1% of peanut.

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Alkaline Phosphatase-Catalyzed Silver Deposition for Electrochemical Detection

Cited by 109 publications

References 33 publications

Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

Electrochemical immunosensor for the analysis of the breast cancer biomarker HER2 ECD

Detection of Ara h 1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensor

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