Alkaline Phosphatase in Human Semen: An Investigation Using Enzyme Inhibitors and Gel Electrophoresis

Lewin, L.; Golan, Kachel; Soffer, Y.; Kaufman, Suzana; Yulzary, Y.; Zaidman, Jacob L.

doi:10.1515/cclm.1993.31.12.811

Cited by 7 publications

(8 citation statements)

References 10 publications

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“…It has been reported that human semen AP was totally inactivated by heating at 56 °C for 15 (Lewin et al ., ); accordingly, the ALP from boar seminal plasma analysed in this article showed a rapid decrease in activity at 60 °C, as already showed by Iyer et al . (), indicating similarity to human semen enzyme and to the bone ALP isoform.…”

Section: Discussionsupporting

confidence: 87%

Alkaline phosphatase in boar sperm function

et al. 2013

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SUMMARYAlkaline phosphatase (AP) catalyses the detachment of phosphate residues from different substrates. Its activity has been demonstrated in seminal plasma and spermatozoa from porcine and other mammalian species; anyway, the role of AP in male reproduction has not been clarified yet and the aim of this study was to determine AP function in boar sperm capacitation and in vitro fertilization (IVF). AP activity was assayed in seminal plasma and in uncapacitated and in vitro capacitated (IVC) spermatozoa; in addition, capacitation was studied in presence of different doses of AP (1.2 and 2.5 IU/mL). The effect of different doses of AP (1.2 and 2.5 IU/mL) on several sperm parameters after IVC (viability, acrosome integrity with FITC-PSA, capacitation status with CTC staining, tyrosine phosphorylation) and on fertilizing ability during IVF were also evaluated. High AP activity was detected in seminal plasma, in particular in sperm-rich fraction; a lower activity was detected in uncapacitated spermatozoa while a significant decrease was evidenced after IVC. Viability was not changed by AP supplementation of the capacitating medium, whereas acrosome integrity and capacitation status were significantly affected by 1.2 and 2.5 doses, with a dose-dependent decrease in acrosome-reacted cells as well as in CTC B pattern displaying cells. As for sperm head protein phosphorylation, a decrease in relative fluorescence was detected in AP 2.5 group, if compared with capacitated one. After IVF, a dose-dependent decrease in penetrated oocytes was recorded, with an increase in monospermic zygote rate. In conclusion, we demonstrated that AP activity decreases under capacitating condition and that addition of AP to spermatozoa during capacitation results in a depression of the capacitating process and IVF. We can infer that AP plays a role in keeping spermatozoa quiescent until they are ejaculated and in modulating the acquisition of the fertilizing ability.

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Section: Discussionsupporting

confidence: 87%

Alkaline phosphatase in boar sperm function

et al. 2013

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“…Moreover, we were also concerned about the status of seminal plasma alkaline phosphatase concentrations in IH-treated dogs. Dog's seminal plasma alkaline phosphatase is produced by the epididymis 6,7) and is found in high concentrations (i.e., Ͼ1000 IU/l) in normal ejaculates. 8) If the seminal plasma has a high alkaline phosphatase level but no spermatozoa, there is testicular spermatogenesis failure.…”

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confidence: 99%

Indenopyridine Hydrochloride Induced Testicular Spermatogenesis Failure with High Seminal Alkaline Phosphatase Levels in Male Dog.

Chang

Fung

Lin

et al. 2002

Biological & Pharmaceutical Bulletin

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“…AP has been found in the seminal plasma of humans (Lewin et al. ) and numerous animal species (Valiente et al. ; Kutzler et al.…”

Section: Discussionmentioning

confidence: 99%

Presence of Membranous Vesicles in Cat Seminal Plasma: Ultrastructural Characteristics, Protein Profile and Enzymatic Activity

Polisca

Troisi

Minelli

et al. 2014

Reprod Domestic Animals

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This study sought to verify the presence of membranous vesicles in cat seminal plasma by means of transmission electron microscopy and to identify protein profile and some of the enzymatic activities associated with these particles. The transmission electron microscopy observations showed the existence of different sized vesicular membranous structures of more or less spherical shape. These vesicles were surrounded by single-, double- or multiple-layered laminar membranes. The vesicle diameters ranged from 16.3 to 387.4 nm, with a mean of 116.5 ± 70.7 nm. Enzyme activity determinations showed the presence of dipeptilpeptidase IV, aminopeptidase, alkaline and acid phosphatase. To our knowledge, this is the first report that identifies and characterizes the membranous vesicles in cat seminal plasma. However, further studies are necessary to identify the exact site of production of these membranous vesicles in the cat male genital tract and to determine their specific roles in the reproductive events of this species.

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Alkaline Phosphatase in Human Semen: An Investigation Using Enzyme Inhibitors and Gel Electrophoresis

Cited by 7 publications

References 10 publications

Alkaline phosphatase in boar sperm function

Alkaline phosphatase in boar sperm function

Indenopyridine Hydrochloride Induced Testicular Spermatogenesis Failure with High Seminal Alkaline Phosphatase Levels in Male Dog.

Presence of Membranous Vesicles in Cat Seminal Plasma: Ultrastructural Characteristics, Protein Profile and Enzymatic Activity

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