Alkaline phosphatase levels of murine pre-osteoblastic cells on anodized and annealed titanium surfaces

Yeniyol, Sinem; Ricci, John L.

doi:10.26650/eor.2018.78387

Cited by 8 publications

(5 citation statements)

References 32 publications

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“…Different methodical assays indicate the same trend of hBM-MSC’s osteogenic response to the osteogenic culture condition, shown by the increased ALP protein expression profile, the stained area of hydroxyapatite, as well as the upregulated gene expression of important osteogenic markers such as ALPL , IBSP , SP7 and SPP1 . ALP, encoded by the ALPL gene, a well-known maker for bone formation and calcification [ 65 ], provides a high phosphate concentration for osteoblasts during bone mineralization [ 66 ]. IBSP encodes for bone sialoprotein, a late stage osteoblast differentiation marker [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

Micro-porous PLGA/β-TCP/TPU scaffolds prepared by solvent-based 3D printing for bone tissue engineering purposes

Hatt,

Wirth,

Ristaniemi

et al. 2023

Regenerative Biomaterials

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The 3D printing process of fused deposition modelling (FDM) is an attractive fabrication approach to create tissue engineered bone substitutes to regenerate large mandibular bone defects, but often lacks desired surface porosity for enhanced protein adsorption and cell adhesion. Solvent-based printing leads to the spontaneous formation of micropores on the scaffold’s surface upon solvent removal, without the need for further post processing. Our aim is to create and characterise porous scaffolds using a new formulation composed of mechanically stable poly(lactic-co-glycol acid) (PLGA) and osteoconductive β-tricalcium phosphate (β-TCP) with and without the addition of elastic thermoplastic polyurethane (TPU) prepared by solvent-based 3D-printing technique. Large scale regenerative scaffolds can be 3D-printed with adequate fidelity and show porosity at multiple levels analysed via micro-computer tomography, scanning electron microscopy and N2 sorption. Superior mechanical properties compared to a commercially available CaP ink are demonstrated in compression, bending, and screw pull out tests. Biological assessments including cell activity assay and live-dead staining prove the scaffold's cytocompatibility. Osteoconductive properties are demonstrated by performing an osteogenic differentiation assay with primary human bone marrow mesenchymal stromal cells. We propose a versatile fabrication process to create porous 3D-printed scaffolds with adequate mechanical stability and osteoconductivity, both important characteristics for segmental mandibular bone reconstruction.

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Section: Discussionmentioning

confidence: 99%

Micro-porous PLGA/β-TCP/TPU scaffolds prepared by solvent-based 3D printing for bone tissue engineering purposes

Hatt,

Wirth,

Ristaniemi

et al. 2023

Regenerative Biomaterials

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“…ALPL is responsible for encoding the membrane-bound ALP. ALP is a commonly used osteogenic marker to show osteogenic differentiation of MSCs ( Yeniyol and Ricci, 2018 ; Trivedi et al, 2020 ) and plays a crucial role in releasing phosphate by cleaving the phosphate ester bond ( Levitt et al, 2022 ). Cells cultured on a phosphate-based material seem to show a delay in ALPL peak expression.…”

Section: Discussionmentioning

confidence: 99%

β-TCP from 3D-printed composite scaffolds acts as an effective phosphate source during osteogenic differentiation of human mesenchymal stromal cells

Hatt,

van der Heide,

Armiento

et al. 2023

Front. Cell Dev. Biol.

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Introduction: Human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) are often combined with calcium phosphate (CaP)—based 3D-printed scaffolds with the goal of creating a bone substitute that can repair segmental bone defects. In vitro, the induction of osteogenic differentiation traditionally requires, among other supplements, the addition of β-glycerophosphate (BGP), which acts as a phosphate source. The aim of this study is to investigate whether phosphate contained within the 3D-printed scaffolds can effectively be used as a phosphate source during hBM-MSC in vitro osteogenesis.Methods: hBM-MSCs are cultured on 3D-printed discs composed of poly (lactic-co-glycolic acid) (PLGA) and β-tricalcium phosphate (β-TCP) for 28 days under osteogenic conditions, with and without the supplementation of BGP. The effects of BGP removal on various cellular parameters, including cell metabolic activity, alkaline phosphatase (ALP) presence and activity, proliferation, osteogenic gene expression, levels of free phosphate in the media and mineralisation, are assessed.Results: The removal of exogenous BGP increases cell metabolic activity, ALP activity, proliferation, and gene expression of matrix-related (COL1A1, IBSP, SPP1), transcriptional (SP7, RUNX2/SOX9, PPARγ) and phosphate-related (ALPL, ENPP1, ANKH, PHOSPHO1) markers in a donor dependent manner. BGP removal leads to decreased free phosphate concentration in the media and maintained of mineral deposition staining.Discussion: Our findings demonstrate the detrimental impact of exogenous BGP on hBM-MSCs cultured on a phosphate-based material and propose β-TCP embedded within 3D-printed scaffold as a sufficient phosphate source for hBM-MSCs during osteogenesis. The presented study provides novel insights into the interaction of hBM-MSCs with 3D-printed CaP based materials, an essential aspect for the advancement of bone tissue engineering strategies aimed at repairing segmental defects.

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“…Overexpression of RUNX2 and high calcium deposits are known to induce differentiation of BMSCs into pre-osteoblasts 43 . Pre-osteoblasts and osteoblasts secrete ALP to provide a high phosphate concentration at the osteoblast cell surface during bone differentiation and synthesize T1Col to facilitate cell attachment and utilize ALP for bone mineralization 44,45 . Moreover, the binding of osteoblasts or BMSCs to T1Col accelerates osteogenic differentiation by triggering pathways such as ERK1/2 signaling 43 .…”

Section: Discussionmentioning

confidence: 99%

Comparison of three different lactic acid bacteria-fermented proteins on RAW 264.7 osteoclast and MC3T3-E1 osteoblast differentiation

Kim,

Song,

Cheon

et al. 2023

Sci Rep

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Osteoporosis is a state of bone weakening caused by an imbalance in osteoblast and osteoclast activity. In this study, the anti-osteoporotic effects of three proteins fermented by lactic acid bacteria (LAB) were assessed. Commercial proteins sodium caseinate (SC), whey protein isolate (WPI), and soy protein isolate (SPI) were fermented by LAB strains for 48 h. The fermented products (F-SC, F-WPI, and F-SPI, respectively) were used in an in vitro osteoclast and osteoblast-like cell model to assess their effects on bone health. Despite no difference in the results of TRAP staining of RANKL-induced osteoclastogenesis, F-WPI and F-SPI were effective in normalizing the altered gene expression of osteoclastogenesis markers such as TRAP, Nfatc1, RANK, and ATP6v0d. F-SPI was also effective in modulating osteoblasts by enhancing the expression of the osteoblastogenesis markers T1Col, Col2a, and OSX to levels higher than those in the SPI group, indicating that protein characteristics could be enhanced through bacterial fermentation. Moreover, these boosted effects of F-SPI may be involved with isoflavone-related metabolism during LAB-fermentation of SPI. These results demonstrate the potential of LAB-fermented proteins as dietary supplements to prevent bone loss. However, further understanding of its effects on balancing osteoblasts and osteoclasts and the underlying mechanisms is needed.

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Alkaline phosphatase levels of murine pre-osteoblastic cells on anodized and annealed titanium surfaces

Cited by 8 publications

References 32 publications

Micro-porous PLGA/β-TCP/TPU scaffolds prepared by solvent-based 3D printing for bone tissue engineering purposes

Micro-porous PLGA/β-TCP/TPU scaffolds prepared by solvent-based 3D printing for bone tissue engineering purposes

β-TCP from 3D-printed composite scaffolds acts as an effective phosphate source during osteogenic differentiation of human mesenchymal stromal cells

Comparison of three different lactic acid bacteria-fermented proteins on RAW 264.7 osteoclast and MC3T3-E1 osteoblast differentiation

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