Alkaline Phosphatase-Strep tag fusion protein binding to streptavidin: resonant mirror studies 1 1Edited by A. R. Fersht

Recombinant Phycobiliproteins

“…9). Such an effect on affinity has also been observed in the dimeric, Strep-tagged E. coli alkaline phosphatase (67).…”

Use of a Biomimetic Peptide in the Design of a Competitive Binding Assay for Biotin and Biotin Analogues

“…This is supported by the data of Hengsakul and Cass, who reported a fusion of Strep-tag, another mimic of biotin, to the C-terminus of BAP. They reported no apparent change in the K m , but they did observe a decrease in V max (21). This decrease was thought to be due to misfolded forms of the conjugate.…”

Optimisation of a multivalent Strep tag for protein detection

“…The first experiments on the multivalent effects of multiple Strep-tag units enhancing streptavidin binding affinity came from Hengsakul and Cass, who showed that, when coupling the original Strep-tag [10,11] to the C-terminus of dimerising alkaline phosphatase, a dissociation constant of 0.5 µM was measured [37]. Though K d values obtained were lower than reported with a single "original" Strep-tag (72 µm) [10], an observation assigned to the presence of two tags in the dimeric phosphatase, direct comparisons cannot be made as experimental conditions in the two studies were distinct.…”