Alkaline pretreatment of spent coffee grounds for oligosaccharides production by mannanase from Bacillus sp. GA2(1)

Wongsiridetchai, Chayaporn; Chiangkham, Watcharaphan; Khlaihiran, Narisara; Sawangwan, Thornthan; Wongwathanarat, Prasert; Charoenrat, Theppanya; Chantorn, Sudathip

doi:10.1016/j.anres.2018.09.012

Cited by 23 publications

(20 citation statements)

References 15 publications

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“…GA2(1). The TLC results also confirm that the amount of reducing sugar was higher, and mannobiose and mannotriose were oligosaccharide products [27].…”

Section: Thin-layer Chromatographymentioning

confidence: 52%

“…TLC was carried out for the detection of hydrolyzed products using TLC silica gel 60 sheets (Merck, Darmstadt, Germany), modified by the method in Wongsiridetchai et al's study [27]. All samples were applied in equal quantities of 5 µl and run with the mobile phase solvent mixture of isopropyl alcohol/ethyl acetate/water in the ratio of 2:2:1.…”

Section: Thin-layer Chromatography (Tlc) Of Hydrolysis Productsmentioning

confidence: 99%

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Extraction of oligosaccharides and phenolic compounds by roasting pretreatment and enzymatic hydrolysis from spent coffee ground

Rizwan¹,

Hasmukh²

2020

J Appl Biol Biotech

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Spent coffee ground (SCG) is the waste generated during the preparation of instant coffee and is the source of industrially valuable organic compounds. In this article, SCG was pretreated by roasting at 150°C for 30 minutes and heated with water at 90°C for extracting carbohydrates and phenolic compounds, after which 1.0% (w/w) β-mannanase was applied for the hydrolysis of pretreated SCG. SCG is characterized in terms of its total sugar content by the anthrone-sulfuric assay and phenolic compounds by Folin−Ciocalteu's procedure. In this study, the total sugar increased by 14.79% (w/w) by the roasting process, and subsequently enzymatic hydrolysis increased the total sugar yield up to 17.43% (w/w) compared to the untreated SCG, i.e., 10.24% (w/w). The reducing sugar was estimated by the dinitrosalicylic acid method and the end product increased to 106.10 (mg Glucose/g) from the initial content 5.32 (mg Glucose/g raw SCG). The total phenolic compound increased to 291.86 (mg Gallic acid/g lyophilized material), which was a 6.39-fold increase compared to the native SCG (45.68 mg Gallic acid/g). These results point to the valuable compounds present in SCG, can be enhanced by combining the roasting pretreatment and enzymatic hydrolysis, and can be utilized in the food and biotech industries.

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“…GA2(1). The TLC results also confirm that the amount of reducing sugar was higher, and mannobiose and mannotriose were oligosaccharide products [27].…”

Section: Thin-layer Chromatographymentioning

confidence: 52%

Section: Thin-layer Chromatography (Tlc) Of Hydrolysis Productsmentioning

confidence: 99%

Extraction of oligosaccharides and phenolic compounds by roasting pretreatment and enzymatic hydrolysis from spent coffee ground

Rizwan¹,

Hasmukh²

2020

J Appl Biol Biotech

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“…In 2014 and 2016, the International Coffee Organization (ICO) estimated that the amount of coffee consumed worldwide was 8.5 and 9.3 billion kg, respectively. Production of coffee results in the generation of spent coffee ground (SCG) which contains lignocellulosic material [ 34 ]. South Africa, Angola, Kenya, Uganda and Ethiopia are the major producers of SCG in Africa.…”

Section: Mannan-containing Waste Biomass Derived From Agro-processingmentioning

confidence: 99%

Enzymatic Conversion of Mannan-Rich Plant Waste Biomass into Prebiotic Mannooligosaccharides

Hlalukana

Magengelele

Malgas

et al. 2021

Foods

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A growing demand in novel food products for well-being and preventative medicine has attracted global attention on nutraceutical prebiotics. Various plant agro-processes produce large amounts of residual biomass considered “wastes”, which can potentially be used to produce nutraceutical prebiotics, such as manno-oligosaccharides (MOS). MOS can be produced from the degradation of mannan. Mannan has a main backbone consisting of β-1,4-linked mannose residues (which may be interspersed by glucose residues) with galactose substituents. Endo-β-1,4-mannanases cleave the mannan backbone at cleavage sites determined by the substitution pattern and thus give rise to different MOS products. These MOS products serve as prebiotics to stimulate various types of intestinal bacteria and cause them to produce fermentation products in different parts of the gastrointestinal tract which benefit the host. This article reviews recent advances in understanding the exploitation of plant residual biomass via the enzymatic production and characterization of MOS, and the influence of MOS on beneficial gut microbiota and their biological effects (i.e., immune modulation and lipidemic effects) as observed on human and animal health.

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“…Pretreatment of spent coffee grounds: SCGs were soaked with NaOH 0.5 N (SCG/NaOH 0.5 N ratio � 1 : 2) for 5 hours at 50°C. en, each sample of the treated SCGs was dried at 80°C in a drying cell for 8 hours and stored in a glass bottle for use in the next analysis [12].…”

Section: Sample Preparationmentioning

confidence: 99%

“…In addition, to increase the applicability of coffee grounds, coffee grounds have been treated with alkalis at different concentrations. is method can change the structure and, under optimal conditions, does not break the basic structure [12]. To improve the mechanical properties and fire retardancy of epoxy materials, environmentally friendly additives have been studied.…”

Section: Introductionmentioning

confidence: 99%

Hybrid Biocomposites Based on Used Coffee Grounds and Epoxy Resin: Mechanical Properties and Fire Resistance

Nguyen

2021

International Journal of Chemical Engineering

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Studies on using biomaterials hybridized with other materials to produce biomaterials have been paid more attention due to their low cost, abundance, renewability, and degradability. Therefore, these materials are ecofriendly and nontoxic to humans. A large number of used coffee grounds (SCGs) are often discarded and replacements are necessary for dealing with environmental problems. This work developed sustainable materials by reusing SCGs. Used coffee grounds were mixed with epoxy resin at different amounts: 30 wt %, 40 wt %, 50 wt %, and 60 wt %. SCGs were treated with 0.5 N NaOH, at SCGs/NaOH ratio of 1 : 2. SEM images showed that the material with 30 wt % SCGs has good compatibility without phase division on the SCGs-epoxy interface. Results of mechanical properties of epoxy composites with 30 wt % SCGs are as follows: tensile strength of 44.81 ± 10 MPa, flexural strength of 80.07 ± 0.16 MPa, compressive strength of 112.56 ± 0.11 MPa, and Izod strength and impact of 8.21 ± 0.19 kJ/m2. In terms of flame-retardant properties, the oxygen index is limited to 20.8% ± 0.20 and the burning rate according to UL94HB is 27.02 ± 0.29 mm/min. The obtained results indicate that it is possible to produce biohybrid composites from epoxy resin and SCGs. This work offers an ecofriendly alternative method to use the waste of the coffee industry. It contributes to improvements of the general characteristics of composites such as mechanical, thermal, and flame-retardant properties. This work proved that SCGs have a high potential to be used in a wide range of composite materials for civil engineering applications.

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Alkaline pretreatment of spent coffee grounds for oligosaccharides production by mannanase from Bacillus sp. GA2(1)

Cited by 23 publications

References 15 publications

Extraction of oligosaccharides and phenolic compounds by roasting pretreatment and enzymatic hydrolysis from spent coffee ground

Extraction of oligosaccharides and phenolic compounds by roasting pretreatment and enzymatic hydrolysis from spent coffee ground

Enzymatic Conversion of Mannan-Rich Plant Waste Biomass into Prebiotic Mannooligosaccharides

Hybrid Biocomposites Based on Used Coffee Grounds and Epoxy Resin: Mechanical Properties and Fire Resistance

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