Alkaline protease for an efficacious rehydration of skin matrix by a novel Bacillus crolab MTCC 5468 in sustainable leather production: a green approach

Ranjithkumar, Ammasi; Victor, John Sundar; Rose, C.; Muralidharan, C.

doi:10.1007/s10529-019-02769-0

Cited by 14 publications

(5 citation statements)

References 13 publications

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“…Application of enzymes in leather processing may help developing eco-benign process that is less harmful to the environment. Considering the high risks associated with conventional leather processing, there is considerable emphasis on developing enzymatic approaches that are cleaner and safer (Kalaikumari et al 2019 ; Ammasi et al 2020 ). Such biobased processes would help reducing emissions.…”

Section: Microbial Proteases and Its Industrial Applicationsmentioning

confidence: 99%

Microbial proteases: ubiquitous enzymes with innumerable uses

Putatunda²,

et al. 2021

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Proteases are ubiquitous enzymes, having significant physiological roles in both synthesis and degradation. The use of microbial proteases in food fermentation is an age-old process, which is today being successfully employed in other industries with the advent of ‘omics’ era and innovations in genetic and protein engineering approaches. Proteases have found application in industries besides food, like leather, textiles, detergent, waste management, agriculture, animal husbandry, cosmetics, and pharmaceutics. With the rising demands and applications, researchers are exploring various approaches to discover, redesign, or artificially synthesize enzymes with better applicability in the industrial processes. These enzymes offer a sustainable and environmentally safer option, besides possessing economic and commercial value. Various bacterial and fungal proteases are already holding a commercially pivotal role in the industry. The current review summarizes the characteristics and types of proteases, microbial source, their current and prospective applications in various industries, and future challenges. Promoting these biocatalysts will prove significant in betterment of the modern world.

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Section: Microbial Proteases and Its Industrial Applicationsmentioning

confidence: 99%

Microbial proteases: ubiquitous enzymes with innumerable uses

Putatunda²,

et al. 2021

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“…Proteases, or more specifically peptidyl-peptide hydrolases (EC 3.4.21–24 and 99), constitute 60–65% of global enzyme market [ 1 , 2 ] on account of their tremendous applications in detergent, tannery, food, pharmaceutical, medical, leather industries and environmental bioremediation [ 3 , 4 ]. Biochemically speaking, they catalyze the hydrolytic cleavage of peptide bonds in proteins partially generating oligopeptides of various sizes and more completely amino acids.…”

Section: Introductionmentioning

confidence: 99%

Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila

Asitok

Ekpenyong

Takon

et al. 2022

Biotechnology Reports

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“…Bacterial and fungal proteases appear to have significant differences in terms of molecular weight and stability to environmental conditions of temperature, pH and ionic strength (Saggu and Mishra 2017). The conditions of temperature and pH are particularly significant in the classification of proteases and have therefore influenced their commercial applications in industries as varied as detergent, tannery, pharmaceutical, leather and environmental nitrogen cycling (Saxena and Singh 2010;Asitok et al 2017;Ammasi et al 2020). Mesophilic proteases are the most abundant but thermostable proteases are more suitable for specialized applications that involve high temperature processing like paper and textile processing (Abusham et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…In terms of pH discrepancy, proteases are categorized as acidic, neutral and alkaline. Alkaline and neutral proteases dominate literature while acid proteases, produced mostly by filamentous fungi, are less frequently encountered (Gupta et al 2002;Razzaq et al 2019;Ammasi et al 2020).…”

Section: Introductionmentioning

confidence: 99%

A Novel Strain of Stenotrophomonas Acidaminiphila Produces Super-active Alkaline Protease During Cassava Effluent Fermentation: Process Optimization, Kinetic Modeling and Scale-up

Asitok

Ekpenyong

Takon

et al. 2021

Preprint

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PurposeMicrobial fermentations for value-added metabolites production are exploited for efficient bioconversion of agro-industrial wastes for the dual purposes of pollution abatement and cost-effectiveness.MethodsIn the present study, the regular 2-level factorial design was employed to screen fermentation parameters that enhance production of a novel alkaline protease by a strain of Stenotrophomonas acidaminiphila using cassava processing effluent as substrate. Data from randomized experiments of central composite rotatable design for improved enzyme activity, guided by path of steepest ascent experiments, were modeled and optimized by response surface methodology (RSM). Shake flask kinetics of production under optimized conditions was modeled by logistic and modified Gompertz models and determinations of maximum specific growth rate, µmax, maximum volumetric rate of substrate consumption, rsm, maximum volumetric rate of biomass formation, rxm and specific yield of product, Yp/x were made.ResultsLogistic model poorly fitted RSM-generated product formation and substrate consumption data. However, biomass formation was accurately fitted (adjusted r2 >99%), with µmax of 0.471 h-1. The modified Gompertz model, on the contrary, more accurately fitted all three major response data with minimal mean squared error. Potential for scale-up of bioprocess evaluated in 5-L bioreactor satisfactorily revealed 8.5-fold more substrate consumption in bioreactor than in shake flask. The 86.76-fold aqueous two-phase system-purified protease had a specific activity of 1416.73 Umg-1 which improved with increasing surfactant concentration.ConclusionThese results suggest significant bioprocess potential for sustainable cassava effluent management and concomitant commercial production of alkaline protease for industrial detergent application.

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Alkaline protease for an efficacious rehydration of skin matrix by a novel Bacillus crolab MTCC 5468 in sustainable leather production: a green approach

Cited by 14 publications

References 13 publications

Microbial proteases: ubiquitous enzymes with innumerable uses

Microbial proteases: ubiquitous enzymes with innumerable uses

Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila

A Novel Strain of Stenotrophomonas Acidaminiphila Produces Super-active Alkaline Protease During Cassava Effluent Fermentation: Process Optimization, Kinetic Modeling and Scale-up

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