Alkaline stress inhibits the growth of Staphylococcus epidermidis by inducing TCA cycle-triggered ROS production

Ohkubo, Tomotaka; Matsumoto, Yasuhiko; Ogasawara, Yuki; Sugita, Takashi

doi:10.1016/j.bbrc.2021.12.053

Cited by 15 publications

(13 citation statements)

References 24 publications

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“…In Staphylococcus epidermidis, the interruption of the TCA cycle resulted in enhanced oxidative stress tolerance, thereby tuning its antibiotic susceptibility. 24 In the present study, the data show that the mutation of gltA is more tolerant to H 2 O 2 and CuOOH than the wild-type strain (Figure 5). Additionally, the expression levels of aphB, katB, and oxyR were significantly increased in the gltA mutant compared to the wild-type strain.…”

Section: ■ Discussionsupporting

confidence: 55%

“…Intermediates of the TCA cycle such as α-ketoglutarate play an essential role in the detoxification of reactive oxygen species. In Staphylococcus epidermidis , the interruption of the TCA cycle resulted in enhanced oxidative stress tolerance, thereby tuning its antibiotic susceptibility . In the present study, the data show that the mutation of gltA is more tolerant to H 2 O 2 and CuOOH than the wild-type strain (Figure ).…”

Section: Discussionsupporting

confidence: 50%

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Citrate Synthase GltA Modulates the 2,4-Diacetylphloroglucinol Biosynthesis of Pseudomonas fluorescens 2P24 and is Essential for the Biocontrol Capacity

Yang,

Yan,

Zhang

et al. 2023

J. Agric. Food Chem.

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Carbon metabolism is critical for microbial physiology and remarkably affects the outcome of secondary metabolite production. The production of 2,4-diacetylphloroglucinol (2,4-DAPG), a bacterial secondary metabolite with a broad spectrum of antibiotic activity, is a major mechanism used by the soil bacterium Pseudomonas fluorescens 2P24 to inhibit the growth of plant pathogens and control disease occurrence. Strain 2P24 has evolved a complex signaling cascade to regulate the production of 2,4-DAPG. However, the role of the central carbon metabolism in modulating 2,4-DAPG production has not been fully determined. In this study, we report that the gltA gene, which encodes citrate synthase, affects the expression of the 2,4-DAPG biosynthesis gene and is essential for the biocontrol capacity of strain 2P24. Our data showed that the mutation of gltA remarkably decreased the biosynthesis of 2,4-DAPG. Consistent with this result, the addition of citrate in strain 2P24 resulted in increased 2,4-DAPG production and decreased levels of RsmA and RsmE. In comparison with the wild-type strain, the gltA mutant was severely impaired in terms of biocontrol activity against the bacterial wilt disease of tomato plants caused by Ralstonia solanacearum. Moreover, the gltA mutant exhibited increased antioxidant activity, and the expression of oxidative, stress-associated genes, including ahpB, katB, and oxyR, was significantly upregulated in the gltA mutant compared to the wild-type strain. Overall, our data indicate that the citrate synthase GltA plays an important role in the production of 2,4-DAPG and oxidative stress and is required for biocontrol capacity.

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Section: ■ Discussionsupporting

confidence: 55%

Section: Discussionsupporting

confidence: 50%

Citrate Synthase GltA Modulates the 2,4-Diacetylphloroglucinol Biosynthesis of Pseudomonas fluorescens 2P24 and is Essential for the Biocontrol Capacity

Yang,

Yan,

Zhang

et al. 2023

J. Agric. Food Chem.

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“…An acidic skin pH is crucial for the activity of epidermal enzymes required for lipid processing and regulating cohesion proteins, thus preserving the stratum corneum and maintaining hydration levels [ 29 , 30 ]. Low skin pH also conserves the commensal skin microflora, which act as a first-line defence against pathogens through direct competition, influencing cutaneous immunity and supporting barrier homeostasis [ 31 , 32 , 33 , 34 ]. A range of immune cell subsets exist in the skin, secreting a plethora of cytokines and chemokines to modulate host response [ 35 ].…”

Section: The Skin Barriermentioning

confidence: 99%

The Skin Microbiome: Current Landscape and Future Opportunities

Smythe

Wilkinson

2023

IJMS

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Our skin is the largest organ of the body, serving as an important barrier against the harsh extrinsic environment. Alongside preventing desiccation, chemical damage and hypothermia, this barrier protects the body from invading pathogens through a sophisticated innate immune response and co-adapted consortium of commensal microorganisms, collectively termed the microbiota. These microorganisms inhabit distinct biogeographical regions dictated by skin physiology. Thus, it follows that perturbations to normal skin homeostasis, as occurs with ageing, diabetes and skin disease, can cause microbial dysbiosis and increase infection risk. In this review, we discuss emerging concepts in skin microbiome research, highlighting pertinent links between skin ageing, the microbiome and cutaneous repair. Moreover, we address gaps in current knowledge and highlight key areas requiring further exploration. Future advances in this field could revolutionise the way we treat microbial dysbiosis associated with skin ageing and other pathologies.

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“…Tissue‐engineered models of human skin infected with Staphylococcus aureus or Pseudomonas aeruginosa revealed heterogeneous pH microenvironments with the skin pH varying from 5 to 9 14 . Alkaline stress inhibited the growth of Staphylococcus epidermidis , a dominant bacterial strain of the human skin microbiota 15 . Larner et al reported that the repeated application of alkaline urine on the arms of normal volunteers resulted in skin irritation 16 .…”

Section: Introductionmentioning

confidence: 99%

Selective detection of urease‐producing bacteria on the genital skin surface in patients with incontinence‐associated dermatitis

Kohta

Koyanagi

Inagaki

et al. 2023

International Wound Journal

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We aimed to investigate the association between the presence of cutaneous urease‐producing bacteria and the development of incontinence‐associated dermatitis (IAD) using an original urea agar medium as a step toward developing advanced preventive measures. In previous clinical assessments, we developed an original urea agar medium to detect urease‐producing bacteria via the medium's colour changes. In a cross‐sectional study, specimens were collected via the swabbing technique at genital skin sites in 52 stroke patients hospitalised in a university hospital. The primary objective was to compare the presence of urease‐producing bacteria between the IAD and no‐IAD groups. Determining the bacterial count was the secondary objective. The prevalence of IAD was 48%. A significantly higher detection rate of urease‐producing bacteria was observed in the IAD group than in the no‐IAD group (P = .002) despite the total number of bacteria being equivalent between them. In conclusion, we discovered that there was a significant association between the presence of urease‐producing bacteria and IAD development in hospitalised stroke patients.

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Alkaline stress inhibits the growth of Staphylococcus epidermidis by inducing TCA cycle-triggered ROS production

Cited by 15 publications

References 24 publications

Citrate Synthase GltA Modulates the 2,4-Diacetylphloroglucinol Biosynthesis of Pseudomonas fluorescens 2P24 and is Essential for the Biocontrol Capacity

Citrate Synthase GltA Modulates the 2,4-Diacetylphloroglucinol Biosynthesis of Pseudomonas fluorescens 2P24 and is Essential for the Biocontrol Capacity

The Skin Microbiome: Current Landscape and Future Opportunities

Selective detection of urease‐producing bacteria on the genital skin surface in patients with incontinence‐associated dermatitis

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