Alkaline treatment of the cellulose fiber affecting membrane column behaviour for high-performance immunoaffinity chromatography

Abstract

The original cellulose fibers and those treated by alkaline solution were both used to prepare the acrylic membranes. The two kinds of membranes were packed into the columns for high-performance immunoaffinity chromatography by the immobilization of protein A on them. It was observed that the alkaline treatment of the cellulose fiber decreased the pressure resistance of the membrane to the mobile phases and greatly increased the accessible volume to the proteins, but affected the adsorption capacity of human IgG on the protein A membrane columns less. There is little difference between those two kinds of membranes on the adsorption capacities of HIgG, which means that the alkaline treatment of the cellulose fiber only significantly changes the void volume inter-membrane, and the porosity and surface area of membrane less. Alkaline treatment of the cellulose fiber reduced the membrane-column efficiency significantly. Some typical examples for the immunoaffinity analysis of IgG from human and dog plasma on the protein A membrane columns are illustrated.

Affinity membrane chromatography for the analysis and purification of proteins

“…The strength of the membrane increases when it is coupled with hydrophilic groups, and decreases with hydrophobic groups. w x Zhou et al 97 have prepared composite cellulose membranes with original cellulose fibers and fibers treated with alkaline solution. It was observed that alkali treatment of the cellulose fiber decreases the pressure resistance of the membrane to the mobile phases and greatly increases the volume accessible to the proteins, but it does not affect the immuno-adsorption capacity of human IgG on Protein A-immobilized membrane columns as much.…”