Alkaliphilus serpentinus sp. nov. and Alkaliphilus pronyensis sp. nov., two novel anaerobic alkaliphilic species isolated from the serpentinite-hosted Prony Bay Hydrothermal Field (New Caledonia)

Postec, Anne; Quéméneur, Marianne; Lecoeuvre, Aurélien; Chabert, Nicolas; Joseph, Manon; Erauso, Gaël

doi:10.1016/j.syapm.2020.126175

Cited by 22 publications

(13 citation statements)

References 67 publications

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“…Natronincolaceae MAG-1138 also lacks any ATP synthase genes, and its genomic content suggests an obligate fermentative lifestyle (supplemental material). Other genera within family Natronincolaceae include Alkaliphilus and Serpentinicella , which have been isolated from the Prony Bay hydrothermal field, which is also associated with serpentinization ( 62 , 63 ).…”

Section: Resultsmentioning

confidence: 99%

Metabolic Strategies Shared by Basement Residents of the Lost City Hydrothermal Field

Brazelton

McGonigle

Motamedi

et al. 2022

Appl Environ Microbiol

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The Lost City hydrothermal field is an iconic example of a microbial ecosystem fueled by energy and carbon from Earth’s mantle. Uplift of mantle rocks into the seafloor can trigger a process known as serpentinization that releases molecular hydrogen (H 2 ) and creates unusual environmental conditions where simple organic carbon molecules are more stable than dissolved inorganic carbon.

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Section: Resultsmentioning

confidence: 99%

Metabolic Strategies Shared by Basement Residents of the Lost City Hydrothermal Field

Brazelton

McGonigle

Motamedi

et al. 2022

Appl Environ Microbiol

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“…Natronincolaceae MAG-1138 also lacks any ATP synthase genes, and its genomic content suggests an obligate fermentative lifestyle ( Supplemental Material ). Other genera within family Natronincolaceae include Alkaliphilus and Serpentinicella , which have been isolated from the Prony Bay hydrothermal field (Mei et al, 2016; Postec et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Metabolic strategies shared by basement residents of the Lost City hydrothermal field

Brazelton

McGonigle

Motamedi

et al. 2022

Preprint

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Alkaline fluids venting from chimneys of the Lost City hydrothermal field flow from a potentially vast microbial habitat within the seafloor where energy and organic molecules are released by chemical reactions within rocks uplifted from Earth's mantle. In this study, we investigated hydrothermal fluids venting from Lost City chimneys as windows into subseafloor environments where the products of geochemical reactions, such as hydrogen (H2), formate, and methane, may be the only available sources of energy for biological activity. Our deep sequencing of metagenomes and metatranscriptomes from these hydrothermal fluids revealed a few key species of archaea and bacteria that are likely to play critical roles in the subseafloor microbial ecosystem. We identified a population of Thermodesulfovibrionales (belonging to phylum Nitrospirae) as a prevalent sulfate-reducing bacterium that may be responsible for much of the consumption of H2 and sulfate in Lost City fluids. Metagenome-assembled genomes (MAGs) classified as Methanosarcinaceae and Candidatus Bipolaricaulota were also recovered from venting fluids and represent potential methanogenic and acetogenic members of the subseafloor ecosystem. These genomes share novel hydrogenases and formate dehydrogenase-like sequences that may be unique to hydrothermal and subsurface alkaline environments where hydrogen and formate are much more abundant than carbon dioxide. The results of this study include multiple examples of metabolic strategies that appear to be advantageous in hydrothermal and subsurface environments where energy and carbon are provided by geochemical reactions.

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“…a hydrothermal alteration that transforms ferromagnesian minerals contained in ultramafic rocks into serpentine minerals [10]). Prokaryotic communities of the PBHF have been studied [11–15] and several anaerobic Clostridiales strains have been isolated and described as new taxa [15–21]. In contrast, microbial communities of ‘La Crouen’ spring had not been studied previously.…”

Section: Full-textmentioning

confidence: 99%

Alkalicella caledoniensis gen. nov., sp. nov., a novel alkaliphilic anaerobic bacterium isolated from ‘La Crouen’ alkaline thermal spring, New Caledonia

Quéméneur

Erauso

Joseph

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel anaerobic, alkaliphilic, mesophilic, Gram-stain-positive, endospore-forming bacterium was isolated from an alkaline thermal spring (42 °C, pH 9.0) in New Caledonia. This bacterium, designated strain LB2T, grew at 25–50 °C (optimum, 37 °C) and pH 8.2–10.8 (optimum, pH 9.5). Added NaCl was not required for growth (optimum, 0–1 %) but was tolerated up to 7 %. Strain LB2T utilized a limited range of substrates, such as peptone, pyruvate, yeast extract and xylose. End products detected from pyruvate fermentation were acetate and formate. Both ferric citrate and thiosulfate were used as electron acceptors. Elemental sulphur, nitrate, nitrite, fumarate, sulphate, sulfite and DMSO were not used as terminal electron acceptors. The two major cellular fatty acids were iso-C15 : 0 and C16 : 0. The genome consists of a circular chromosome (3.7 Mb) containing 3626 predicted protein-encoding genes with a G+C content of 36.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolate is a member of the family Proteinivoraceae , order Clostridiales within the phylum Firmicutes . Strain LB2T was most closely related to the thermophilic Anaerobranca gottschalkii LBS3T (93.2 % 16S rRNA gene sequence identity). Genome-based analysis of average nucleotide identity and digital DNA–DNA hybridization of strain LB2T with A. gottschalkii LBS3T showed respective values of 70.8 and 13.4 %. Based on phylogenetic, genomic, chemotaxonomic and physiological properties, strain LB2T is proposed to represent the first species of a novel genus, for which the name Alkalicella caledoniensis gen. nov., sp. nov. is proposed (type strain LB2T=DSM 100588T=JCM 30958T).

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Alkaliphilus serpentinus sp. nov. and Alkaliphilus pronyensis sp. nov., two novel anaerobic alkaliphilic species isolated from the serpentinite-hosted Prony Bay Hydrothermal Field (New Caledonia)

Cited by 22 publications

References 67 publications

Metabolic Strategies Shared by Basement Residents of the Lost City Hydrothermal Field

Metabolic Strategies Shared by Basement Residents of the Lost City Hydrothermal Field

Metabolic strategies shared by basement residents of the Lost City hydrothermal field

Alkalicella caledoniensis gen. nov., sp. nov., a novel alkaliphilic anaerobic bacterium isolated from ‘La Crouen’ alkaline thermal spring, New Caledonia

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