Alkaloid production by somatic embryo cultures of Corydalis ambigua

Hiraoka, Noboru; Bhatt, Indra D.; Sakurai, Yuko; Chang, Jung-In

doi:10.5511/plantbiotechnology.21.361

Cited by 20 publications

(6 citation statements)

References 29 publications

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“…Consequently, it is quite probable that the callus propagated in this study lacked the levels of cellular and/or subcellular differentiation required for alkaloid production, but this limitation was overcome by the callus differentiating into plantlets via somatic embryogenesis. Similar examples can be found in the scientific literature for different alkaloids and plant species, e.g ., the MIA umbellatine in Psychotria umbellata or the protoberberine isoquinoline alkaloids corydaline and cavidine in Corydalis ambigua …”

Section: Resultsmentioning

confidence: 99%

“…Similar examples can be found in the scientific literature for different alkaloids and plant species, e.g., the MIA umbellatine in Psychotria umbellata [17] or the protoberberine isoquinoline alkaloids corydaline and cavidine in Corydalis ambigua. [18] Whether or not the growth regulators added to the culture medium had an impact on alkaloid production in the callus remains unclear. On the one hand, 2,4-D has been proposed to reduce alkaloid synthesis in in vitro cultures.…”

Section: Quantification Of Ibogaine and Voacangine In Alkaloid Extracmentioning

confidence: 99%

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Quantification of Anti‐Addictive Alkaloids Ibogaine and Voacangine in In Vivo‐ and In Vitro‐Grown Plants of Two Mexican Tabernaemontana Species

Krengel

Santoyo

Flores

et al. 2016

Chemistry & Biodiversity

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Tabernaemontana alba and Tabernaemontana arborea are Apocynaceae species used in Mexican traditional medicine for which little phytochemical information exists. In this study, preliminary gas chromatography/mass spectrometry analyses of different organs obtained from wild plants of both species identified a total of 10 monoterpenoid indole alkaloids (MIAs) and one simple indole alkaloid, nine of which were reported for the first time in these species. Furthermore, callus cultures were established from T. alba leaf explants and regeneration of whole plants was accomplished via somatic embryogenesis. The anti-addictive MIAs ibogaine and voacangine were then quantified by gas chromatography with flame ionization detection in wild plants of both species, as well as greenhouse-grown plants, in vitro-grown plantlets and embryogenic callus of T. alba. Ibogaine and voacangine were present in most samples taken from the whole plants of both species, with stem and root barks showing the highest concentrations. No alkaloids were detected in callus samples. It was concluded that T. alba and T. arborea are potentially viable sources of ibogaine and voacangine, and that these MIAs can be produced through somatic embryogenesis and whole plant regeneration of T. alba. Approaches to increase MIA yields in whole plants and to achieve alkaloid production directly in cell cultures are discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Quantification Of Ibogaine and Voacangine In Alkaloid Extracmentioning

confidence: 99%

Quantification of Anti‐Addictive Alkaloids Ibogaine and Voacangine in In Vivo‐ and In Vitro‐Grown Plants of Two Mexican Tabernaemontana Species

Krengel

Santoyo

Flores

et al. 2016

Chemistry & Biodiversity

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“…The bioreactor RITA® was of interest for the production of ginsenosid and betalains, respectively, by the culture of Panax ginseng roots and Beta vulgaris hairy roots . Similarly, thidiazuron enhanced alkaloid production by somatic embryo culture of Corydalis ambigua .…”

Section: Resultsmentioning

confidence: 99%

Leucojum aestivum plants propagated in in vitro bioreactor culture and on solid media containing cytokinins

Ptak

Simlat

Kwiecień

et al. 2012

Engineering in Life Sciences

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Cytokinins are growth regulators that stimulate cell division and control morphogenesis in plants, however their role in regulating secondary metabolism is not well studied. The influence of various cytokinins (benzyladenine, zeatin, kinetin, meta‐topolin, thidiazuron) and culture systems (solid and temporary immersion RITA® system) on the quality Leucojum aestivum plant regenerated from somatic embryos was investigated. The largest number of regenerated plants (181.6 and 168.8) was obtained from the embryos cultivated on media enriched with meta‐topolin and benzyladenine. Thidiazuron and meta‐topolin led to the highest number of normally developed plants (94.8 and 90.6). The random amplified polymorphic DNA analysis of in vitro and in vivo plants showed four clusters of similarity. The highest biomass (growth index: 2.49) was obtained with the temporary immersion RITA® system. Alkaloid extracts were analyzed by LC‐MS, leading to the quantification of galanthamine and lycorine both in plant materials and in liquid media. The highest contents of galanthamine (0.05% dry weight) were observed in plants cultivated in the presence of thidiazuron in bioreactor system. Galanthamine was accumulated (highest content 0.05% dry weight) in plants cultivated in the presence of thidiazuron in bioreactor system whereas lycorine was synthetized mainly in plants cultivated on solid media.

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“…Besides, reports on secondary metabolite production by somatic embryo culture in Ammi vishaga (Apiaceae) provide an encouraging lead for trying the same in A. glauca. This could effectively reduce the pressure of raw material from pharmaceutical houses (Hiraoka et al, 2004). In this regard, it is further encouraging that the amount of secondary metabolites produced by somatic embryos is proportional or sometimes higher than the potential plant parts in some cases (Kaul and Staba, 1967).…”

Section: Introductionmentioning

confidence: 99%

Note on somatic embryogenesis and synthetic seed production in Angelica glauca: A valuable medicinal plant of Himalaya

Bisht¹,

Bhatt²,

Dhar³

2015

J. Med. Plants Res.

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This is the first report of somatic embryogenesis and synthetic seed production in Angelica glauca, a valuable medicinal plant of Himalaya. Somatic embryogenesis protocol was developed using leaf explants from newly sprouted rhizomes. Mature leaf explants were inoculated in Murishige and Skoog (MS) medium supplemented with 3 µM 2,4-dichlorophenoxyacetic acid (2,4-D) induced 86% callus. Calli (0.5 g) subcultured into different concentration of 1-napthaleneacetic (NAA) and 6-benzylaminopurine (BA) either alone or in combination produced globular structure and their differentiation. NAA (2 µM) in combination of BA (2 µM) germinated 2.2 shoots per culture in 8 weeks time. Plantlets when transferred into sand, soil and peat moss (1:1:1) ratio were found suitable for acclimatization and 75% plantlets survived. Somatic embryos were put into a combination of 3% (w/v) sodium alginate and 100 µM calcium nitrate for 30 min for protecting the somatic embryos and the production of synthetic seeds. Experiments on the evaluation of synthetic seeds are under progress.

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Alkaloid production by somatic embryo cultures of Corydalis ambigua

Cited by 20 publications

References 29 publications

Quantification of Anti‐Addictive Alkaloids Ibogaine and Voacangine in In Vivo‐ and In Vitro‐Grown Plants of Two Mexican Tabernaemontana Species

Quantification of Anti‐Addictive Alkaloids Ibogaine and Voacangine in In Vivo‐ and In Vitro‐Grown Plants of Two Mexican Tabernaemontana Species

Leucojum aestivum plants propagated in in vitro bioreactor culture and on solid media containing cytokinins

Note on somatic embryogenesis and synthetic seed production in Angelica glauca: A valuable medicinal plant of Himalaya

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