Alkaloid production from regenerated Solanum plants

El-Ashaal, Hanan A; Ghanem, S. A.; Melek, F. R.; Kohail, M.A; Hilal, S. H.

doi:10.1016/s0367-326x(99)00069-6

Cited by 9 publications

(9 citation statements)

References 8 publications

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“…On contrast to the present results, it was reported that auxins and cytokinins were essential for root development in S. torvum cultures [35]. Shoots of S. nigrum cultures were found to grow successfully in MS media containing Indole-3-butyric acid and 1-naphthaleneacetic acid, but at cytokinin: auxin ratio (1:2) with roots in hormone free MS media [14].…”

Section: Discussioncontrasting

confidence: 55%

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In vitro produced glycoalkaloids from Solanum nigrum L. and evaluation of their potential role as antibilharziasis

Al-Ashaal¹,

Abduallah²,

Farghaly³

et al. 2017

APJTD

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Section: Discussioncontrasting

confidence: 55%

“…The challenge of producing natural antibilharziasis drugs from plant origin lead to searching for alternative way rather than breeding for producing such valuable compounds. Different studies of in vitro glycoalkaloids production from Solanum species are reported [13][14][15][16].…”

Section: A Rt I C L E I N F O Abstractmentioning

confidence: 99%

In vitro produced glycoalkaloids from Solanum nigrum L. and evaluation of their potential role as antibilharziasis

Al-Ashaal¹,

Abduallah²,

Farghaly³

et al. 2017

APJTD

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“…The production of alkaloids in plant tissue culture by some solanaceous species have been presented in many reports as shown in Table 1 (Yamada & Hashimoto, 1982;Conner, 1987;Savary & Dougall, 1990;El-Ashaal et al, 1999;Khanam et al, 2000;Khanam et al, 2001;Okršlar et al, 2002;Kang et al, 2004;Jacob & Malpathak, 2005a;Jacob & Malpathak, 2005b;Bhat et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Solasodine accumulation in regenerated plants of Solanum torvum Sw

Moreira

Lima

Esquibel

et al. 2010

Rev. bras. plantas med.

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RESUMO: Acúmulo de solasodina em plantas micropropagadas de Solanum torvum Sw. A regeneração de Solanum torvum e a avaliação do conteúdo de solasodina foram os objetivos de cultura de segmentos nodais. A influência de auxinas (ácido 3-indolacético, ácido naftalenoacético) e de 6-benzilaminopurina no crescimento de S. torvum na micropropagação foi investigado. Cultura de segmentos nodais foi iniciada por sementes germinadas em meio básico MS acrescido de GA 3 e cultivadas em diferentes concentrações de AIA, AIA + BAP e ANA + BAP. Plantas da cultura in vitro com 60 dias foram coletadas, congeladas e liofilizadas e o método de alaranjado de metila foi utilizado para quantificação de solasodina para o ensaio espectrofotométrico. Os melhores resultados para regeneração vegetal e acúmulo de solasodina foram alcançados no meio MS sem adição de reguladores de crescimento havendo, porém grande produção de calos de base friáveis. Esses resultados mostram que a partir desses calos, cultura de células pode ser iniciada com aplicação de outras auxinas e citocininas para o aumento da produção de solasodina além de diferentes eliciadores, intensidades luminosas e concentrações de sacarose.Palavras-chave: Solanaceae, jurubeba, auxina, citocinina, alcalóide esteroidal ABSTRACT: A nodal segment culture was developed in order to assess Solanum torvum Sw. regeneration and solasodine levels. The influence of auxins (indoleacetic acid, 1-Naphthaleneacetic acid) and benzyl adenine on S. torvum growth in micropropagation was investigated. A nodal segment culture was initiated with seeds germinated in MS basal medium added of GA 3 and grown in different concentrations of IAA, IAA + BAP and NAA + BAP. Sixty-day-old plants from the in vitro culture were collected, frozen and lyophilized; then, the methyl orange method was used to quantify solasodine for the spectrophotometric assay. The best results regarding plant regeneration and solasodine accumulation were obtained by using the MS basal medium without addition of plant growth regulators; however, there was great production of calluses presenting friable bases. Based on these results, cell cultures can be initiated from such calluses with application of other auxins and cytokinins to enhance solasodine production, besides different elicitors, light intensities and sucrose concentrations.

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“…(Lech et al 1996;Jabeen et al 2005). Four of the studies (Baburaj and Thamizhchelvan 1991;El-Ashaal et al 1999;Okrslar et al 2002;Hussain et al 2005) showed that BA was the most succesful growth regulator for Solanum spp., like bittersweet.…”

Section: Resultsmentioning

confidence: 99%

“…f. (Baburaj and Thamizhchelvan 1991), Solanum commersonii Dun. (Iapichino et al 1991), Solanum tuberosum L. (Yadav and Sticklen 1995;Nadolska-Orczyk et al 1995;Hussain et al 2005), Solanum nigrum L. (El-Ashaal et al 1999), Solanum laciniatum Ait. (Okrslar et al 2002) and Lycopersicon spp.…”

Section: Resultsmentioning

confidence: 99%

Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant

Türker

Multu

2011

Acta Soc Bot Pol

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Solanum dulcamara L. (bittersweet) is a medicinal plant that has been used to treat skin diseases, warts, tumors, felons, arthritis, rheumatism, bronchial congestion, heart ailments, ulcerative colitis, eye inflammations, jaundice and pneumonia. A reliable in vitro culture protocol for bittersweet was established. Explants (leaf and petiole segments) were cultured on Murashige and Skoog minimal organics (MSMO) medium with various plant growth regulator combinations. Leaf explants formed more shoots than petiole explants. Plant regeneration was observed through indirect organogenesis with both explants. Best shoot proliferation was obtained from leaf explants with 3 mg/l BA (benzyladenine) and 0.5 mg/l IAA (indole-3-acetic acid). Regenerated shoots were transferred to rooting media containing different levels of IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), NAA (naphthalene acetic acid) or 2,4-D (2,4 dichlorophenoxyacetic acid). Most shoots developed roots on medium with 0.5 mg/l IBA. Rooted explants were transferred to vermiculate in Magenta containers for acclimatization and after 2 weeks, they were planted in plastic pots containing potting soil and maintained in the plant growth room.

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Alkaloid production from regenerated Solanum plants

Cited by 9 publications

References 8 publications

In vitro produced glycoalkaloids from Solanum nigrum L. and evaluation of their potential role as antibilharziasis

In vitro produced glycoalkaloids from Solanum nigrum L. and evaluation of their potential role as antibilharziasis

Solasodine accumulation in regenerated plants of Solanum torvum Sw

Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant

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