Alkaloids and Phenolics ofColchicum cilicicum^1,2

Abstract

The distributions of the alkaloids colchicine, 2-demethylcolchicine, 3-demethylcolchicine, demecolcine, 2-demethyldemecolcine, and 3-demethyldemecolcine in the corms, stems, leaves, flowers, and seeds of COLCHICUM CILICICUM were analysed by HPLC. In addition, the alkaloids cornigerine, N-formyl- N-deacetylcolchicine, N-methyl-demecolcine, beta-lumicolchicine, gamma-lumicolchicine, beta-lumidemecolcine, gamma-lumidemecolcine, and N-ethoxycarbonyldemecolcine were identified, the last named being reported for the first time from a COLCHICUM species. The flavones luteolin and apigenin were isolated and benzoic, 2-hydroxy-6-methoxybenzoic, and vanillic acids were also detected.

Chemical constituents of the different parts ofColchicum baytopiorumand their cytotoxic activities on K562 and HL60 cell lines

“…Previous studies have indicated that the composition of tropolone alkaloids differs in different parts of Colchicum species and varies during the different growth stages (Sütlüpınar et al, 1988;Husek et al, 1990;Alali et al, 2006). The aim of the present work was to determine the chemical constituents of the different parts (perigon, perigon tube, leaf, seed, and corm) of C. baytopiorum, an unexplored Turkish species, as a continuation of our studies on Colchicum species (Baytop & Özcöbek, 1970;Baytop et al, 1980;Sütlüpınar, 2002).…”

Colchicinoids fromColchicum crocifoliumBoiss. (Colchicaceae)

“…(Colchicaceae) was processed into three fractions (A-C) according to the schemes of Santavy, Preininger, Simanek, and Potesilova (1981) and Sutlupinar et al (1988). A high concentration of colchicinoids was noted in the TLC of fraction C by distinctive yellow spots after spraying with 5% phosphomolybdic acid in EtOH.…”

“…Dried plant material (537.7 g) was extracted with MeOH in a Soxhlet apparatus for 3 h. The solvent was evaporated under reduced pressure to yield a MeOHextract (148.1 g), which was fractionated based on the methods of Santavy et al (1981) and Sutlupinar et al (1988). Briefly, the MeOH-extract was dissolved in 5% acetic acid, extracted with light petroleum (Fraction A) (12.0 g), after which the aqueous acid residue was re-extracted three times with diethyl ether (Fraction B) (1.2 g).…”

A New Paradigm for Personalized Medicine and Companion Diagnostics: The Contract Diagnostics Organization

“…This experiment was conducted to detect the effect of sucrose at different concentrations 0.0, 0.1, 0.2, 0.3 M on alkaloid production. Callus was subcultured to the MS media supplemented with different concentrations and data collected every other week for colchicine content using high performance liquid chromatography (HPLC) [18].…”

“…Several studies in the literature were reported to study (-)-colchicine content in pharmaceutical preparations, biological fluids and plant extracts [5,[16][17][18][19]. Production of colchicine alkaloid by plant tissue cultures was first investigated in suspension cultures of Colchicum autumnale [20,21].…”