Alkane utilisation by Cladosporium resinae: the importance of extended lag phases when assessing substrate optima

Lindley, Nic D.

doi:10.1016/0378-1097(85)90054-0

Cited by 1 publication

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“…Ions with ranging from m/z 700 to 800 were detected, with two major ion species appearing at m/z 734 [M + Li] + and 750 [M + Li] + (Figure 5 A). Although in a different ratio, similar results were found in the analysis of glycosphingolipids from Cladosporium resinae (Figure 5 B), a saprophytic species commonly found as a contaminant in fuel oil storage tanks (Lindley and Heyderman, 1985 ; Seifert et al, 2007 ). CID-MS analysis confirmed their similarities and revealed that the differences were located in the LCB moieties (Figures 6 A–D).…”

Section: Analytical Techniques To Study Cerebrosidessupporting

confidence: 77%

Structural Analysis of Fungal Cerebrosides

Barreto‐Bergter¹,

Sassaki²,

Souza³

2011

Front. Microbio.

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Of the ceramide monohexosides (CMHs), gluco- and galactosyl-ceramides are the main neutral glycosphingolipids expressed in fungal cells. Their structural determination is greatly dependent on the use of mass spectrometric techniques, including fast atom bombardment-mass spectrometry, electrospray ionization, and energy collision-induced dissociation mass spectrometry. Nuclear magnetic resonance has also been used successfully. Such a combination of techniques, combined with classical analytical separation, such as high-performance thin layer chromatography and column chromatography, has led to the structural elucidation of a great number of fungal CMHs. The structure of fungal CMH is conserved among fungal species and consists of a glucose or galactose residue attached to a ceramide moiety containing 9-methyl-4,8-sphingadienine with an amidic linkage to hydroxylated fatty acids, most commonly having 16 or 18 carbon atoms and unsaturation between C-3 and C-4. Along with their unique structural characteristics, fungal CMHs have a peculiar subcellular distribution and striking biological properties. Fungal cerebrosides were also characterized as antigenic molecules directly or indirectly involved in cell growth or differentiation in Schizophyllum commune, Cryptococcus neoformans, Pseudallescheria boydii, Candida albicans, Aspergillus nidulans, Aspergillus fumigatus, and Colletotrichum gloeosporioides. Besides classical techniques for cerebroside (CMH) analysis, we now describe new approaches, combining conventional thin layer chromatography and mass spectrometry, as well as emerging technologies for subcellular localization and distribution of glycosphingolipids by secondary ion mass spectrometry and imaging matrix-assisted laser desorption ionization time-of-flight.

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Section: Analytical Techniques To Study Cerebrosidessupporting

confidence: 77%