Alkylglycerol monooxygenase, a heterotaxy candidate gene, regulates left-right patterning via Wnt signaling

Duncan, Anna R.; González, Delfina P; Viso, Florencia Del; Robson, Andrew; Khokha, Mustafa K.; Griffin, John N.

doi:10.1016/j.ydbio.2019.07.019

Cited by 6 publications

(4 citation statements)

References 77 publications

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“…The gene and the protein expressions of AGMO (encoded by BGIBMGA002524 ) were significantly higher in the silk glands of the jNC1, kC and h75c strains with heavier silk gland phenotypes, suggesting that the gene may promote the silk gland weight and the silk yield. Previous research has demonstrated that AGMO promotes lipid metabolism, infection prevention, nematode life extension (Samuelson et al, 2007; Watschinger & Werner, 2013) and the proper growth of the left‐right (LR) axis of the Xenopus embryo (Duncan et al, 2019). This result suggests that AGMO may also be crucial for the symmetrical development of the silk gland organs in a developing silkworm.…”

Section: Discussionmentioning

confidence: 99%

Identification of genes associated with the silk gland size using multi‐omics in silkworm (Bombyx mori)

Sun,

Chen

et al. 2023

Insect Molecular Biology

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Silk gland size in silkworms (Bombyx mori) affects silk output. However, the molecular mechanisms by which genes regulate silk gland size remain unclear. In this study, silk glands from three pure silkworm strains (A798, A306 and XH) with different silk gland weight phenotypes were compared using transcriptomics and proteomics to identify differentially expressed genes (DEGs) and proteins (DEPs). When comparing A798 to A306 and A798 to XH, 830 and 469 DEGs were up‐regulated, respectively. These genes were related to the gene ontology terms, metabolic process, transport activity and biosynthesis process. In addition, 372 and 302 up‐regulated differentially expressed proteins were detected in A798 to A306 and A798 to XH, respectively, related to the gene ontology terms, ribosome and protein export, ribosome and polypeptide biosynthesis processes. Moreover, combined transcriptomics, proteomics and weighted correlation network analyses showed that five genes (BGIBMGA002524, BGIBMGA002629, BGIBMGA005659, BGIBMGA005711 and BGIBMGA010889) were significantly associated with the silk gland weight. Reverse Transcription‐quantitative real‐time Polymerase Chain Reaction (RT‐qPCR) and Enzyme linked immunosorbent assay (ELISA) were used to verify the mRNA and protein expression of five genes in the silk glands and tissues of 18 silkworm strains. The results showed that four genes have higher expression levels in heavier silk glands. These genes are associated with glycogen metabolism, fatty acid synthesis and branched chain amino acid metabolism, thus potentially promoting growth and silk protein synthesis. These findings provide valuable insights into the molecular mechanisms underlying the relationship between silk gland weight and silk yield in silkworms.

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Section: Discussionmentioning

confidence: 99%

Identification of genes associated with the silk gland size using multi‐omics in silkworm (Bombyx mori)

Sun,

Chen

et al. 2023

Insect Molecular Biology

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“…Little is also known about the role AGMO may play in cancer, which primarily functions to aid in the synthesis of membrane lipids. However, recent studies have indicated a potential role for this gene in the regulation of Wnt secretion [50,51]. Correct regulation of Wnt signaling is vital to the maintenance of healthy rates of stem cell proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

Controlling for cellular heterogeneity using single-cell deconvolution of gene expression reveals novel markers of colorectal tumors exhibiting microsatellite instability

Devall

Casey

2021

Oncotarget

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Approximately 15% of colorectal cancer (CRC) cases present with high levels of microsatellite instability (MSI-H). Bulk RNA-sequencing approaches have been employed to elucidate transcriptional differences between MSI-H and microsatellite stable (MSS) CRC tumors. These approaches are frequently confounded by the complex cellular heterogeneity of tumors. We performed single-cell deconvolution of bulk RNA-sequencing on The Cancer Genome Atlas colon adenocarcinoma (TCGA-COAD) dataset. Cell composition within each dataset was estimated using CIBERSORTx. Cell composition differences were analyzed using linear regression. Significant differences in abundance were observed for 13 of 19 cell types between MSI-H and MSS/MSI-L tumors in TCGA-COAD. This included a novel finding of increased enteroendocrine ( q = 3.71E -06 ) and reduced colonocyte populations ( q = 2.21E -03 ) in MSI-H versus MSS/MSI-L tumors. We were able to validate some of these differences in an independent biopsy dataset. By incorporating cell composition into our regression model, we identified 3,193 differentially expressed genes ( q = 0.05), of which 556 were deemed novel. We subsequently validated many of these genes in an independent dataset of colon cancer cell lines. In summary, we show that some of the challenges associated with cellular heterogeneity can be overcome using single-cell deconvolution, and through our analysis we highlight several novel gene targets for further investigation.

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“…Alterations in Wnt pathway proteins are associated with developmental abnormalities and cancers. 14,15 The pathways play a key role in kidney development 16 and nephron formation 17,18 . Alterations in Wnt/β-catenin signaling is involved in congenital defects of the kidney and urinary tracts, renal carcinoma, obstructive nephropathy, chronic allograft nephropathy, diabetic nephropathy, polycystic kidney disease, focal and segmental glomerulosclerosis, and adriamycin nephropathy 20-23, 19, 24 .…”

Section: Introductionmentioning

confidence: 99%

Wnt/β-Catenin Pathway Proteins in End-Stage Renal Disease

2021

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Aim: To delineate the association of end-stage renal disease (ESRD) and Wnt-proteins including the agonist R-spondin-1, the transducer β-catenin and the antagonists DKK1 and sclerostin. Materials & methods: Serum Wnt-pathway proteins levels were measured by ELISA in 60 ESRD patients and 30 normal controls. Results: DKK1 and sclerostin were significantly higher in ESRD than in controls, and β-catenin and the catenin + R-sponin-1/DKK1 + sclerostin ratio, reflecting the ratio of agonist and transducer on antagonists (AT/ANTA), were significantly lower in ESRD. Estimated glomerular filtration rate was significantly associated with DKK1 and sclerostin (inversely), β-catenin (positively) and the AT/ANTA ratio (r = 0.468, p < 0.001). Conclusion: Wnt/β-catenin pathway proteins show significant alterations in ESRD, indicating significantly increased levels of antagonists.

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Alkylglycerol monooxygenase, a heterotaxy candidate gene, regulates left-right patterning via Wnt signaling

Cited by 6 publications

References 77 publications

Identification of genes associated with the silk gland size using multi‐omics in silkworm (Bombyx mori)

Identification of genes associated with the silk gland size using multi‐omics in silkworm (Bombyx mori)

Controlling for cellular heterogeneity using single-cell deconvolution of gene expression reveals novel markers of colorectal tumors exhibiting microsatellite instability

Wnt/β-Catenin Pathway Proteins in End-Stage Renal Disease

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