All-aqueous core-shell droplets produced in a microfluidic device

Ziemecka, Iwona; Steijn, Volkert van; Koper, Ger J. M.; Kreutzer, Michiel T.; Esch, Jan H. van

doi:10.1039/c1sm06517c

Cited by 98 publications

(98 citation statements)

References 20 publications

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“…droplet, which is in turn dispersed in a third fluid that serves as a continuous phase. 13,16 We focus on the latter as the starting point in our work. Such "drop-in-drop" structures have also attracted significant interest in the context of aqueous-organic emulsification with capillary microfluidics, 17 and their internal structure can be understood by invoking well-established equilibrium criteria.…”

Section: Introductionmentioning

confidence: 99%

Tunable spatial heterogeneity in structure and composition within aqueous microfluidic droplets

et al. 2012

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In this paper, we demonstrate biphasic microfluidic droplets with broadly tunable internal structures, from simple near-equilibrium drop-in-drop morphologies to complex yet uniform non-equilibrium steady-state structures. The droplets contain an aqueous mixture of poly(ethylene glycol) (PEG) and dextran and are dispensed into an immiscible oil in a microfluidic T-junction device. Above a certain welldefined threshold droplet speed, the inner dextran-rich phase is "stirred" within the outer PEG-rich phase. The stirred polymer mixture is observed to exhibit a near continuum of speed and composition-dependent phase morphologies. There is increasing interest in the use of such aqueous two-phase systems in microfluidic devices for biomolecular applications in a variety of contexts. Our work presents a method to go beyond equilibrium phase morphologies in generating microfluidic "multiple" emulsions and at the same time raises the possibility of biochemical experimentation in benign yet complex biomimetic milieus. V C 2012 American Institute of Physics. [http://dx

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Section: Introductionmentioning

confidence: 99%

Tunable spatial heterogeneity in structure and composition within aqueous microfluidic droplets

et al. 2012

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“…37 The average diameter of the resulting droplets, D drop , is related to the forcing frequency through the expression D drop ¼ (6Q in /pf) 1/3 . 29 When the frequency of perturbation exceeds the critical value, f p *, the growth rate of Rayleigh-Plateau's instability on a perturbed jet diminishes or even becomes null. Consequently, perturbation only induces corrugations, which do not grow in amplitude or break up into droplets.…”

Section: A Hydrodynamic Perturbation Induced Droplet Formationmentioning

confidence: 99%

“…This water/water interface is sensitive to the vibrations 28,79 in the surrounding environment as long as the viscosities are not exceedingly large. To take advantage of this property, mechanical perturbations have been introduced to oscillate the jet phase, inducing breakup of the jet, 28,29 as shown in Figures 1(a)-1(c). Other phenomena may also arise as the properties of the fluids change.…”

Section: Microfluidic Manipulation On the All-aqueous Jetsmentioning

confidence: 99%

“…These vibrations can be incorporated into the microfluidic devices by embedding a piezoelectric actuator in the channel wall 29,35 or by using a mechanical vibrator that squeezes the soft tubing connected to the incoming channel. 28,36 The vibrator imposes fluctuations to the driving pressure and modulates the instantaneous flow rate of the perturbed phase, changing locally the diameters of the w/w jet.…”

Section: A Hydrodynamic Perturbation Induced Droplet Formationmentioning

confidence: 99%

“…All-aqueous emulsions with structures of higher complexity can be generated based on the perturbation approach. A w/w/w double emulsion can be prepared by oscillating a w/w/w compound jet 29 or applying multiple stages of emulsification. 36 The number and diameters of the innermost liquid cores are adjusted by varying the frequency of perturbation and flow rates.…”

Section: A Hydrodynamic Perturbation Induced Droplet Formationmentioning

confidence: 99%

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All-aqueous multiphase microfluidics

Song¹,

Sauret²,

Shum³

2013

Biomicrofluidics

101

104

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Immiscible aqueous phases, formed by dissolving incompatible solutes in water, have been used in green chemical synthesis, molecular extraction and mimicking of cellular cytoplasm. Recently, a microfluidic approach has been introduced to generate all-aqueous emulsions and jets based on these immiscible aqueous phases; due to their biocompatibility, these all-aqueous structures have shown great promises as templates for fabricating biomaterials. The physico-chemical nature of interfaces between two immiscible aqueous phases leads to unique interfacial properties, such as an ultra-low interfacial tension. Strategies to manipulate components and direct their assembly at these interfaces needs to be explored. In this paper, we review progress on the topic over the past few years, with a focus on the fabrication and stabilization of all-aqueous structures in a multiphase microfluidic platform. We also discuss future efforts needed from the perspectives of fluidic physics, materials engineering, and biology for fulfilling potential applications ranging from materials fabrication to biomedical engineering.

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In Situ Endothelialization of Free‐Form 3D Network of Interconnected Tubular Channels via Interfacial Coacervation by Aqueous‐in‐Aqueous Embedded Bioprinting

et al. 2022

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factors as well as get rid of waste products. [7] Three-dimensional (3D) bioprinting holds great promise as a versatile tool for replicating the spatial complexity of vascularized tissues. [8,9] For example, coaxial extrusion bioprinting can be used to fabricate cell-laden hollow microfibers that function as perfusable lumens. However, 3D networks of interconnected perfusable lumens with structural complexity is as yet difficult to achieve. [10][11][12][13] Both 3D extrusion bioprinting with fugitive inks and digital light processing (DLP)-based bioprinting enable the production of 3D networks of interconnected channels, which can be post-seeded with endothelial cells. [14][15][16] However, both processes involve multiple steps including template removal, repeated cleansing, and post-seeding. [17][18][19] These post-procedures could influence the structural precision, and forfeit inherent advantages of 3D additive manufacturing, such as rapid-prototyping and simplicity. More importantly, most templated materials used in these approaches, such as Pluronic F-127 and carbohydrate glass, are either unsuitable for carrying cells, or highly toxic to cells. [20][21][22] Thus, these approaches do not support in situ seeding of cells, which requires high seeding efficiency and uniformity. [18] To circumvent the challenge of achieving both structural printability and in situ cell seeding, embedded bioprinting is

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All-aqueous core-shell droplets produced in a microfluidic device

Cited by 98 publications

References 20 publications

Tunable spatial heterogeneity in structure and composition within aqueous microfluidic droplets

Tunable spatial heterogeneity in structure and composition within aqueous microfluidic droplets

All-aqueous multiphase microfluidics

In Situ Endothelialization of Free‐Form 3D Network of Interconnected Tubular Channels via Interfacial Coacervation by Aqueous‐in‐Aqueous Embedded Bioprinting

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