All-electrochemical nanocomposite two-electrode setup for quantification of drugs and study of their electrocatalytical conversion by cytochromes P450

Shumyantseva, Victoria V.; Булко, Т. В.; Kuzikov, Alexey V.; Masamrekh, Rami A.; Konyakhina, Apollinariya Yu; Romanenko, Iuliia; Max, Johannes B.; Köhler, Moritz; Gilep, Andrei A.; Usanov, Sergey A.; Pergushov, Dmitry V.; Schacher, Felix H.; Sigolaeva, Larisa V.

doi:10.1016/j.electacta.2019.135579

Cited by 35 publications

(29 citation statements)

References 52 publications

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“…Polymeric materials of amphiphilic nature can provide good dispersibility of MWCNTs in aqueous media and ensure long-term colloidal stability of such systems, as we demonstrated earlier for polymers of various structure and composition, such as imidazolium-based poly(ionic liquid)s [ 23 ], cationic poly(1,2-butadiene)- block -poly(2-(dimethylamino)ethyl methacrylate) diblock copolymers [ 34 ], anionic poly( n -butylacrylate)- block -poly(acrylic acid) diblock copolymers [ 36 ], and poly(dehydroalanine)-based graft copolymers [ 38 ]. The amphiphilic nature of the polymer is of key importance for successful dispersing of superhydrophobic carbon nanomaterials to individual fragments (particles) in aqueous media.…”

Section: Resultsmentioning

confidence: 99%

“…Based on potentiometric titration data and our previous experience [ 34 ], we assume that due to their pronounced amphiphilic character P n BMA x - b -PDMAEMA y diblock copolymers will easily disperse carbon nanomaterials such as MWCNTs. Furthermore, the best colloidal stability of dispersions is expected in acidic media where PDMAEMA blocks are in their charged (protonated) state.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, the best colloidal stability of dispersions is expected in acidic media where PDMAEMA blocks are in their charged (protonated) state. However, one cannot obtain homogeneous dispersions of MWCNTs by simple mixing of MWCNT powder with a solution of P n BMA x - b -PDMAEMA y at pH 3 (corresponding to the fully protonated PDMAEMA blocks) [ 23 , 34 , 36 ]. Only after high energy ultrasound treatment, the disentanglement of MWCNTs bundles to individual MWCNTs was done, and we obtained very homogenous black suspensions (“inks”), which were colloidally stable at room temperature for at least several months (> 7 months).…”

Section: Resultsmentioning

confidence: 99%

“…However, MWCNTs are hardly dispersible in most conventional solvents, and that makes them not easy-to-handle. The main strategy to overcome this disadvantage is dispersing of MWCNTs in solutions of synthetic or natural polymers [ 23 , 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…Due to their amphiphilic structure, such materials are good dispersants for carbon nanomaterials. Moreover, the charged groups provide the subsequent immobilization of analytes, while the presence of a hydrophobic segment preferably with a low or moderate glass transition temperature T g might considerably facilitate the modification of hydrophobic carbon surfaces [ 23 , 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

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Rational Design of Amphiphilic Diblock Copolymer/MWCNT Surface Modifiers and Their Application for Direct Electrochemical Sensing of DNA

et al. 2020

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We demonstrate the application of amphiphilic ionic poly(n-butylmethacrylate)-block- poly(2-(dimethylamino)ethyl methacrylate) diblock copolymers (PnBMA40-b-PDMAEMA40, PnBMA40-b-PDMAEMA120, PnBMA70-b-PDMAEMA120) for dispersing multiwalled carbon nanotubes (MWCNTs) in aqueous media, a subsequent efficient surface modification of screen-printed electrodes (SPEs), and the application of the modified SPEs for DNA electrochemistry. Stable and fine aqueous dispersions of MWCNTs were obtained with PnBMAx-b-PDMAEMAy diblock copolymers, regardless of the structure of the copolymer and the amount of MWCNTs in the dispersions. The effect of the diblock copolymer structure was important when the dispersions of MWCNTs were deposited as modifying layers on surfaces of SPEs, resulting in considerable increases of the electroactive surface areas and great acceleration of the electron transfer rate. The SPE/(PnBMAx-b-PDMAEMAy + MWCNT) constructs were further exploited for direct electrochemical oxidation of the guanine (G) and adenine (A) residues in a model salmon sperm double-stranded DNA (dsDNA). Two well-defined irreversible oxidation peaks were observed at about +600 and +900 mV, corresponding to the electrochemical oxidation of G and A residues, respectively. A multi-parametric optimization of dsDNA electrochemistry enables one to get the limits of detection (LOD) as low as 5 μg/mL (0.25 μM) and 1 μg/mL (0.05 μM) for G and A residues, respectively. The achieved sensitivity of DNA assay enables quantification of the A and G residues of dsDNA in the presence of human serum and DNA in isolated human leukocytes.

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