All-in-one strategy for the fabrication of antimicrobial biomimetic films on stainless steel

Abstract

International audienceHere we report on an all-in-one approach to prepare robust antimicrobial films on stainless steel. The strategy is based on the layer-by-layer deposition of polyelectrolytes. A polycationic copolymer bearing 3,4-dihydroxyphenylalanine units (DOPA, a major component of natural adhesives) was synthesized and co-deposited with precursors of silver nanoparticles as the first layer. The presence of DOPA units ensures a strong anchoring on the stainless steel substrate, and the silver nanoparticles are sources of biocidal Ag+, providing stainless steel with antimicrobial activity. We show that multilayered films, obtained by alternating this nanoparticle-loaded polycationic copolymer with polystyrene sulfonate, a commercial polyanion, results in stainless steel with high antibacterial activity against Gram-negative E. coli bacteria. The polycationic layers are a reservoir of Ag+ that can be reactivated after depletion. The whole process of film formation, including the synthesis of the copolymer, is conducted in aqueous media under very mild conditions, which makes it very attractive for industrial scale-up and sustainable applications

Antibacterial activity of poly(vinyl alcohol)-b-poly(acrylonitrile) based micelles loaded with silver nanoparticles

“…2). The appearance of absorption bands at this wavelength indicates the formation of silver nanoparticles [22].…”

“…The aqueous solution of the copolymer loaded by silver nanoparticles was efficiently used for imparting antibacterial activity to steel [22]. In this work the DOPA moieties of the copolymer were responsible for the reduction of silver nitrate into elemental silver and for their stabilization by chelation.…”

Surface engineering of polymer membranes via mussel-inspired chemistry

“…Considering the strong adhesion of catechol structure, we can design catecholamine grafted polymers as membrane modifiers, by conjugating DOPA or dopamine onto the polymer chains through covalent bonds. Numerous dopamine-or DOPA-conjugated polymers have been synthetized for various applications [171]. For example, dopamine was conjugated to heparin via EDC/NHS reaction.…”

Diblock copolymer micelles as surface-functionalized particles and direct decoration of nanoparticles on their surface

“…There have been various reports on dopaminefunctionalized diblock copolymers [23e27], including the synthesis based on activated esters [28e30]. For example, formation of metal nanoparticles on the dopamine units of diblock copolymers was exploited [27]. In addition, RAFT-polymerized block copolymers having a dopamine-containing anchor block as a solubilizing block for functionalization of TiO 2 nanorods was reported [31].…”