ALL Maintenance Treatment for Early Loss of B-Cell Aplasia after Tisagenlecleucel Therapy

Abstract

Chimeric antigen receptor (CAR) T-cell therapy is a new, effective treatment for patients with relapsed/refractory (r/r) B-cell acute lymphoblastic leukaemia (ALL). Tisagenlecleucel achieved a complete remission (CR) rate and minimal residual disease (MRD) negativity of 81% at 3 months in the pivotal study; overall survival (OS) was 76% at 12 months (Maude et al, 2018). Real world data confirmed similar outcomes, with 1-year OS of 77% and event free survival (EFS) of 52% (Pasquini et al, 2020). Relapse can occur in the form of CD19 negative or CD19 positive ALL. The latter is associated with lack of persistence of the CAR T product. B-cell aplasia (BCA) is an indirect measure of CAR T presence. Early (<6 months from infusion) loss of BCA is associated with high relapse risk (Pillai et al, 2019); therefore, allogeneic stem cell transplantation (SCT) is often considered. However, SCT is associated with therapy-related morbidity and mortality and not all patients will find a suitable donor. Therefore, the optimal management of patients with loss of BCA is yet to be defined. In our centre, we administered maintenance therapy to a cohort of children with early loss of BCA. When compared to UK patients undergoing SCT for the same indication, we noted promising early outcomes. We report the findings here. We collected data on children with r/r ALL treated with tisagenlecleucel at Great Ormond Street Hospital (GOSH) from January 2018 to January 2021 who presented loss of BCA without evidence of disease (negative molecular or flow cytometry MRD) within 12 months from infusion. Loss of BCA was defined as peripheral B-cell count ≥0.10 x 10^9/L or bone marrow (BM) CD19+ events ≥0.1%. We compared outcomes of children who received maintenance as per UKALL 2011 protocol at GOSH to those who received SCT for the same indication from all UK paediatric centres. Fourteen patients from GOSH met the inclusion criteria. Four had loss of BCA after 6 months from CAR T infusion, none of them received additional therapy and they are all alive and in CR at a median of 535 days after CAR T infusion (Figure 1, A and B). Ten patients recovered B cells at <6 months: 3 proceeded to SCT, 6 started on maintenance therapy, 1 received other treatment. In 2 cases, maintenance was commenced after a second CAR T infusion. Two patients from the UK cohort met the inclusion criteria for the SCT group. Analysis was performed on 6 children who received maintenance and 5 who had SCT. Baseline characteristics of the 2 groups were similar (male/female ratio, median age at infusion, cytogenetics). Time from infusion to loss of BCA did not differ: the median was 80 days (range 28-168) in children who had maintenance vs 93 days (range 28-150) in those who had SCT. At a median follow up of 511 days (range 222-812), 3/6 children who received maintenance relapsed at median 210 days after infusion and proceeded to further treatment, no patient relapsed post SCT. One child died of disease in the maintenance group 237 days after infusion, 2 children died of transplant related mortality in the SCT group at 222 and 422 days post infusion. OS and EFS did not differ statistically between the 2 groups, as shown in Figure 1 (C and D). We observed that outcome for patients who presented loss of BCA within or at 2 months from infusion was poor regardless of the intervention (maintenance or SCT). Management of patients who experience early loss of BCA after CAR T is challenging and there are little data to support optimal treatment. In our experience, maintenance therapy compared favourably with SCT with similar rate of OS and EFS. Of note, 2/5 patients died of TRM in the SCT group highlighting the toxicity of this approach in such heavily pre-treated patients. On the other hand, maintenance is a well tolerated, low-cost treatment which can be easily delivered on an out-patient basis. Our preliminary data support investigation of this strategy in larger, prospectively-recruited cohorts of patients. Moreover, our preliminary data suggest that the time of loss of BCA is a crucial clinical parameter, as children who developed it within 2 months from infusion had the worst outcome, possibly reflecting prior therapy intensity and its impact on autologous T cells. Figure 1 Figure 1. Disclosures Amrolia: ADC Therapeutics: Other: Named inventor on a patent which is being transferred to ADCT.; Autolus: Patents & Royalties. Ghorashian: UCLB: Patents & Royalties: CARPALL; Novartis: Honoraria.

Determinants of outcomes and advances in CD19‐directed chimeric antigen receptor therapy for B‐cell acute lymphoblastic leukemia

“…No toxicity was noted with the use of pembrolizumab. There is currently an ongoing Phase II clinical trial evaluating a second (early) reinfusion of tisagenlecleucel in children and AYA with B‐ALL to evaluate if this can keep participants in BCA (REFUEL study, NCT05460533).In a study from the United Kingdom 46 looking at the use of maintenance therapy in patients with loss of BCA within 12 months of tisagenlecleucel infusion versus alloHSCT, of the 14 enrolled patients, four had loss of BCA after 6 months from CAR‐T infusion. None of them received additional therapy, and they are all alive and in CR at a median of 535 days after CAR‐T infusion.…”

“…In a study from the United Kingdom 46 looking at the use of maintenance therapy in patients with loss of BCA within 12 months of tisagenlecleucel infusion versus alloHSCT, of the 14 enrolled patients, four had loss of BCA after 6 months from CAR‐T infusion. None of them received additional therapy, and they are all alive and in CR at a median of 535 days after CAR‐T infusion.…”

“…A pharmacodynamic assessment of CAR‐T cell function by the persistence of BCA is superior to direct assessment by flow cytometry or PCR 4 . Loss of BCA has been defined as peripheral B‐cell count ≥0.10 × 10 9 /L or bone marrow CD19+ events ≥0.1% 46 . Loss of BCA is associated with T‐cell exhaustion and CAR permanence and is associated with a high risk of relapse 47 .…”

“…4 Loss of BCA has been defined as peripheral B-cell count ≥0.10 Â 10 9 /L or bone marrow CD19+ events ≥0.1%. 46 Loss of BCA is associated with T-cell exhaustion and CAR permanence and is associated with a high risk of relapse. 47 Higher CD19 antigen burden has also been shown to be associated with early loss of BCA.…”

Novel cellular immunotherapies for hematological malignancies: recent updates from the 2021 ASH annual meeting

“…Early (< 6 months from infusion) loss of B cell aplasia (BCA) is associated with high relapse risk [ 10 ]. In a single center experience, the maintenance therapy after CD19 CAR-T treatment achieved similar rate of overall survival (OS) and event-free survival (EFS) while being better-tolerated and lower-cost compared to HSCT in pediatric patients with B-ALL [ 11 ].…”