All-optical measurements of the bending rigidity of lipid-vesicle membranes across structural phase transitions

Lee, Chau Hwang; Lin, Wan Chen; Wang, Jyhpyng

doi:10.1103/physreve.64.020901

Cited by 181 publications

(92 citation statements)

References 31 publications

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“…3 [30,35]. Note that, as both temperatures above T m are also above the anomalous swelling regime, no softening of the bending modulus is expected.…”

Section: Prl 109 058102 (2012) P H Y S I C a L R E V I E W L E T T Ementioning

confidence: 92%

Lipid Bilayers and Membrane Dynamics: Insight into Thickness Fluctuations

et al. 2012

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Thickness fluctuations have long been predicted in biological membranes but never directly observed experimentally. Here, we utilize neutron spin echo spectroscopy to experimentally reveal such fluctuations in a pure, fully saturated, phosphocholine lipid bilayer system. These fluctuations appear as an excess in the dynamics of undulation fluctuations. Like the bending rigidity, the thickness fluctuations change dramatically as the lipid transition temperature is crossed, appearing to be completely suppressed below the transition. Above the transition, the relaxation rate is on the order of 100 ns and is independent of temperature. The amplitude of the thickness fluctuations is 3:7 # A AE 0:7 # A, which agrees well with theoretical calculations and molecular dynamics simulations. The dependence of the fluctuations on lipid tail lengths is also investigated and determined to be minimal in the range of 14 to 18 carbon tails.

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“…3 [30,35]. Note that, as both temperatures above T m are also above the anomalous swelling regime, no softening of the bending modulus is expected.…”

Section: Prl 109 058102 (2012) P H Y S I C a L R E V I E W L E T T Ementioning

confidence: 92%

Lipid Bilayers and Membrane Dynamics: Insight into Thickness Fluctuations

et al. 2012

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“…Recently a few new methods have been developed for measuring the bending rigidity of membranes in the gel phase (Dimova et al 2000b, Lee et al 2001, Mecke et al 2003. For the first time the temperature dependence of κ of a membrane in the gel phase was measured using optical dynamometry (Dimova et al 2000b, Dimova andPouligny 2006).…”

Section: Bending Rigidity Of Membranes In the Gel Phasementioning

confidence: 99%

A practical guide to giant vesicles. Probing the membrane nanoregime via optical microscopy

Dimova

Aranda

Bezlyepkina

et al. 2006

J. Phys.: Condens. Matter

300

321

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Research on giant vesicles is becoming increasingly popular. Giant vesicles provide model biomembrane systems for systematic measurements of mechanical and rheological properties of bilayers as a function of membrane composition and temperature, as well as hydrodynamic interactions. Membrane response to external factors (for example electric fields, ions and amphiphilic molecules) can be directly visualized under the microscope. In this paper we review our current understanding of lipid bilayers as obtained from studies on giant unilamellar vesicles. Because research on giant vesicles increasingly attracts the interest of scientists from various backgrounds, we also try to provide a concise introduction for newcomers in the field. Finally, we summarize some recent developments on curvature effects induced by polymers, domain formation in membranes and shape transitions induced by electric fields.

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“…The change of these material parameters can be followed through phase transitions (68) and their dependence on membrane composition can be studied (57). The use of artificial membranes for elucidating lipid driven heterogeneity was spurred by the work of Dietrich et al (60,69).…”

Section: Formation and Observationmentioning

confidence: 99%