All-trans retinoic acid regulates sheep primary myoblast proliferation and differentiation in vitro

Li, Q.; Zhang, T.; Zhang, R.; Qin, Xuze; Zhao, Junxing

doi:10.1016/j.domaniend.2019.106394

Cited by 17 publications

(9 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RA, a metabolite of vitamin A, is an important factor involved in cell growth and differentiation [ 41 ]. Currently, there is increasing evidence that RA inhibits cell proliferation and promotes cell differentiation in different tissues or cells [ 27 , 42 – 44 ]. More importantly, BHLHE40, a transcriptional repressor, has been shown to be a target gene of RA in P19 cells [ 45 ], which is consistent with the sequencing results of this study.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Song,

Zhao,

Zhang

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Background Vitamin A and retinoic acid (RA, a metabolite of vitamin A), are inextricably involved to the development of skeletal muscle in animals. However, the mechanisms regulating skeletal muscle development by vitamin A remain poorly reported. The current study designed to investigate the underlying mechanism of vitamin A affecting myogenic differentiation of lamb myoblasts through transcriptome sequencing (RNA-Seq) and gene function validation experiments. It provides a theoretical basis for elucidating the regulation of vitamin A on skeletal muscle development as well as for improving the economic benefits of the mutton sheep industry. Results Newborn lambs were injected with 7,500 IU vitamin A, and longissimus dorsi (LD) muscle tissue was surgically sampled for RNA-Seq analysis and primary myoblasts isolation at 3 weeks of age. The results showed that a total of 14 down-regulated and 3 up-regulated genes, were identified between control and vitamin A groups. Among them, BHLHE40 expression was upregulated in vitamin A group lambs. Furthermore, BHLHE40 expression is significantly increased after initiation of differentiation in myoblasts, and RA addition during differentiation greatly promoted BHLHE40 mRNA expression. In vitro, RA inhibited myoblasts proliferation and promoted myoblasts myogenic differentiation through BHLHE40. Moreover, BHLHE40 was proved to inhibit the expression of the DNA binding inhibitor 3 (ID3), and meanwhile, ID3 could effectively promote myoblasts proliferation and inhibit myoblasts myogenic differentiation. Conclusions Taken together, our results suggested that vitamin A inhibited myoblasts proliferation and promoted myoblasts myogenic differentiation by inhibiting ID3 expression through BHLHE40.

show abstract

Section: Discussionmentioning

confidence: 99%

“…For studies of primary myoblasts differentiation, the medium containing 20% FBS was replaced with differentiation medium containing 2% horse serum. Based on previous study [ 27 ], the retinoic acid concentration in subsequent cell experiments was 100 nM.…”

Section: Methodsmentioning

confidence: 99%

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Song,

Zhao,

Zhang

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…Among them, the "Notch signaling pathway" [50,51], "PI3K-Akt signaling pathway" [52], and "Hippo signaling pathway" [53,54], as well as the "focal adhesion" [55], "tight adhesion" [56], and "cell cycle" [57] pathways, were confirmed to regulate muscle proliferation and differentiation. Metabolic pathways, including the "citrate cycle" [58]; "biosynthesis of unsaturated fatty acids" [59]; "sphingolipid metabolism" [60]; "retinol metabolism" [61,62]; "valine, leucine and isoleucine degradation" [63]; and "thyroid hormone synthesis" [35,64] pathways, are closely associated with muscle development. Notably, these pathways were not enriched in the 15-month-old vs. 3-month-old comparison.…”

Section: Discussionmentioning

confidence: 99%

Integrated Transcriptome Analysis of miRNAs and mRNAs in the Skeletal Muscle of Wuranke Sheep

Yun,

Wu,

et al. 2023

Genes

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are regarded as important regulators in skeletal muscle development. To reveal the regulatory roles of miRNAs and their target mRNAs underlying the skeletal muscle development of Wuranke sheep, we investigated the miRNA and mRNA expression profiles in the biceps femoris of these sheep at the fetal (3 months of gestation) and 3- and 15-month-old postnatal stages. Consequently, a total of 1195 miRNAs and 24,959 genes were identified. Furthermore, 474, 461, and 54 differentially expressed miRNAs (DEMs) and 6783, 7407, and 78 differentially expressed genes (DEGs) were detected among three comparative groups. Functional analysis demonstrated that the target mRNAs of the DEMs were enriched in multiple pathways related to muscle development. Moreover, the interactions among several predicted miRNA–mRNA pairs (oar-miR-133-HDAC1, oar-miR-1185-5p-MYH1/HADHA/OXCT1, and PC-5p-3703_578-INSR/ACTG1) that potentially affect skeletal muscle development were verified using dual-luciferase reporter assays. In this study, we identified the miRNA and mRNA differences in the skeletal muscle of Wuranke sheep at different developmental stages and revealed that a series of candidate miRNA–mRNA pairs may act as modulators of muscle development. These results will contribute to future studies on the function of miRNAs and their target mRNAs during skeletal muscle development in Wuranke sheep.

show abstract

“…Retinoids, a group of compounds including vitamin A and its active metabolite ATRA, regulate serial physiological activity in multiple organ systems, such as cell growth, differentiation, and apoptosis ( 36 – 38 ). Indeed, differentiation therapy with ATRA has marked a major advance, and ATRA has become the first-choice drug in the treatment of acute promyelocytic leukemia ( 39 – 42 ).…”

Section: Discussionmentioning

confidence: 99%

A Retinol Derivative Inhibits SARS-CoV-2 Infection by Interrupting Spike-Mediated Cellular Entry

Tong

Wang

Liao

et al. 2022

mBio

View full text Add to dashboard Cite

Retinoids, a group of compounds including vitamin A and its active metabolite all- trans retinoic acid (ATRA), regulate serial physiological activity in multiple organ systems, such as cell growth, differentiation, and apoptosis. The ATRA analogues reported to date include more than 4,000 natural and synthetic molecules that are structurally and/or functionally related to ATRA.

show abstract

All-trans retinoic acid regulates sheep primary myoblast proliferation and differentiation in vitro

Cited by 17 publications

References 39 publications

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Integrated Transcriptome Analysis of miRNAs and mRNAs in the Skeletal Muscle of Wuranke Sheep

A Retinol Derivative Inhibits SARS-CoV-2 Infection by Interrupting Spike-Mediated Cellular Entry

Contact Info

Product

Resources

About