All-trans retinoic acid regulates the expression of apolipoprotein E in rats with glomerulosclerosis induced by Adriamycin

Zhou, Tian-Biao; Qin, Yue; Lei, Feng-Ying; Su, Li-Na; Zhao, Yanyan; Huang, Wei-Fang

doi:10.1016/j.yexmp.2011.03.001

Cited by 32 publications

(24 citation statements)

References 44 publications

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“…The absorbance was measured using an ultraviolet spectrophotometer, and agarose gel electrophoresis confirmed that there had been no degradation of RNA by visualizing the 18S and 28S RNA bands under ultraviolet light. 40,41 Primers were designed according to primer design principles by Primer Premier 5.0 (Premier Biosoft International, Palo Alto, CA, USA). The primers for PAX2 and internal control β-actin were as follows: F 5 -AAGCGACAGAACCCGACTATGT-3 and R 5 -ACTCCTGTCCCTGCCCCAT-3 for PAX2; F 5 -GCCCCTGAGGAGCACCCTGT-3 and R 5 -ACGCTCGGTCAGGATCTTCA-3 for β-actin.…”

Section: Real-time Reverse Transcription Polymerase Chain Reaction Tomentioning

confidence: 99%

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Association of PAX2 with Cell Apoptosis in Unilateral Ureteral Obstruction Rats

et al. 2012

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Renal interstitial fibrosis (RIF) is the final common pathway for chronic kidney disease. Cell apoptosis is a critical detrimental event that leads to renal fibrosis. Paired box 2 (PAX2) plays a major role in the development of the kidney. This study was performed to investigate whether PAX2 was associated with cell apoptosis in the progression of RIF in unilateral ureteral obstruction (UUO) rats. Eighty Wistar male rats were divided into two groups randomly: sham operation group (SHO) and model group subjected to UUO (GU), n = 40, respectively. The model was established by left ureteral ligation. Renal tissues were collected 14 and 28 days after surgery. Protein expressions of PAX2, transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (α-SMA), collagen-IV (Col-IV), fibronectin (FN), and caspase-3 were detected using immunohistochemical analysis; mRNA expression of PAX2 in renal tissue was detected by real-time reverse transcription polymerase chain reaction; and RIF index and cell apoptosis index in renal interstitium were also calculated. When compared with those in the SHO group, expressions of PAX2 (protein and mRNA) were markedly increased in the GU group (each p < 0.01). Protein expressions of TGF-β1, α-SMA, Col-IV, FN, and caspase-3 and RIF index and cell apoptosis index in the GU group were remarkably increased when compared with those in the SHO group (each p < 0.01). The protein expression of PAX2 was positively correlated with the protein expressions of TGF-β1, α-SMA, Col-IV, FN, and caspase-3 and with RIF index and cell apoptosis index (all p < 0.01). The apoptotic cell in our observation was mainly derived from renal tubular epithelial cells. In conclusion, the increased expression of PAX2 is associated with cell apoptosis in the progression of RIF in UUO rats, suggesting that PAX2 is a potentially therapeutic target for prevention of RIF.

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Section: Real-time Reverse Transcription Polymerase Chain Reaction Tomentioning

confidence: 99%

“…The data for fold change were analyzed using 2 − Ct . 41,42 For example, the Ct for PAX2 mRNA expression in the GU group on the 14th day was as follows:…”

Section: Real-time Reverse Transcription Polymerase Chain Reaction Tomentioning

confidence: 99%

Association of PAX2 with Cell Apoptosis in Unilateral Ureteral Obstruction Rats

et al. 2012

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“…There were some studies which reported that ATRA could play a protective role in the prevention of renal diseases. Our previous study 14 found that the ATRA could alleviate the glomerulosclerosis lesion in rats. Kishimoto et al conducted an interesting investigation and reported that ATRA had a preventive effect on renal fibrosis induced by UUO.…”

Section: Discussionmentioning

confidence: 94%

“…Ultraviolet spectrophotometer measuring absorbance, agarose gel electrophoresis confirmed that there had been no degradation of RNA by visualizing the 18S and 28S RNA bands under ultraviolet light. 14,15 Primers were designed according to primer design principles by Primer Premier 5.0. The primers for Smad3 and internal control β-actin were as follows: F 5 0 -CTGGCTACCTGAGTGAAGATG-3 0 and R 5 0 -TGTGAAGCGTGGAATGTCTC-3 0 for Smad3; F 5 0 -GCCCCTGAGGAGCACCCTGT-3 0 and R 5 0 -ACGCTCGGTCAGGATCTTCA-3 0 for β-actin.…”

Section: Real Time Reverse Transcription Polymerase Chain Reaction Tomentioning

confidence: 99%

All-Trans Retinoic Acid Attenuates the Renal Interstitial Fibrosis Lesion in Rats but Not By Transforming Growth Factor-β1/Smad3 Signaling Pathway

Zhou

Qin

et al. 2012

Renal Failure

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All-trans retinoic acid (ATRA) is an important therapeutic agent for prevention of the renal diseases. Transforming growth factor-β1 (TGF-β1)/Smad3 signaling pathway is a key signaling pathway which takes part in the progression of renal interstitial fibrosis (RIF). This investigation was performed to study the effect of ATRA in RIF rats and its effect on the TGF-β1/Smad3 signaling pathway. Sixty Wistar male rats were divided into three groups at random: sham operation group (SHO), model group subjected to unilateral ureteral obstruction (GU), model group treated with ATRA (GA), n ¼ 20, respectively. RIF index, protein expression of TGF-β1, collagen-IV (Col-IV) and fibronectin (FN) in renal interstitium, and mRNA and protein expressions of Smad3 in renal tissue were detected at 14-day and 28-day after surgery. The RIF index was markedly elevated in group GU than in SHO group (p < 0.01), and the RIF index of GA group was alleviated when compared with that in GU group (p < 0.01). Compared with in group SHO, the mRNA/protein expression of Smad3 in renal tissue was significantly increased in group GU (p < 0.01). However, the mRNA and protein expressions of Smad3 in renal tissue in GA group were not markedly alleviated by ATRA treatment when compared with those in GU (each p > 0.05). Protein expressions of TGF-β1, Col-IV, and FN in GU group were markedly increased than those in SHO group (each p < 0.01), and their expressions in GA group were markedly down-regulated by ATRA treatment than those of GU group (all p < 0.01). The protein expression of Smad3 was positively correlated with RIF index, protein expression of TGF-β1, Col-IV or FN (each p < 0.01). In conclusion, ATRA treatment can alleviate the RIF progression in UUO rats. However, ATRA cannot affect the signaling pathway of TGF-β1/Smad3 in the progression of RIF.

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“…The average threshold cycle (Ct, the cycles of template amplification to the threshold) was worked out as the value of each sample. The data for fold change was analyzed using 2 −ΔΔCt [19,20].…”

Section: Real Time Reverse Transcription Polymerase Chain Reaction Tomentioning

confidence: 99%

Effect of Ginkgo biloba extract on the expressions of Cox-2 and GST-Pi in rats with hepatocellular carcinoma risk

Zhao

et al. 2014

Afr H. Sci.

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All-trans retinoic acid regulates the expression of apolipoprotein E in rats with glomerulosclerosis induced by Adriamycin

Cited by 32 publications

References 44 publications

Association of PAX2 with Cell Apoptosis in Unilateral Ureteral Obstruction Rats

Association of PAX2 with Cell Apoptosis in Unilateral Ureteral Obstruction Rats

All-Trans Retinoic Acid Attenuates the Renal Interstitial Fibrosis Lesion in Rats but Not By Transforming Growth Factor-β1/Smad3 Signaling Pathway

Effect of Ginkgo biloba extract on the expressions of Cox-2 and GST-Pi in rats with hepatocellular carcinoma risk

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