Allele frequencies of 13 short tandem repeats in population samples from the Iberian Peninsula and Northern Africa

Abstract

The 13 short tandem repeat (STR) loci D3S1358, vWA, FGA, D16S539, TH01, TPOX, CSF1PO, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820 as well as the amelogenin locus, contained in AmpFlSTR Profiler Plus and/or AmpFlSTR Cofiler and/or AmpFlSTR Green I PCR amplification kits, were studied in four populations from the Iberian Peninsula, Basques, Catalans, Andalusians and Portuguese and two North African populations (Moroccan Arabs and Berbers). The aim of the study was to obtain accurate allele frequency data and other genetic parameters of forensic interest on the main representative human groups living in Iberia and Morocco using an automated method and commercial amplification kits.

Microsatellite studies on an isolated population of African descent in the Brazilian state of Bahia

“…There were no individuals classified as Amerindian or Caucasian by phenotype in this commuStudies in an African-descendant isolate 25 Sources: (1) Africans - (Deka et al, 1999;Destro-Bisol et al, 2000;Huguet et al, 1998;Bosh et al, 2001;Gene et al, 2001;Alves et al, 2001;Brinkmann et al, 1996;Brinkmann et al, 1998;Rolf et al, 1998;Wang et al, 2002;Pérez-Lezaun et al, 1997;Yunis et al, 2000;Corte-Real et al, 2000;Dios et al, 1998); (2) Europeans (Pérez- Lezaun et al, 2000;Anjos et al, 2000;Lourdes-Pontes et al, 1998;Asmundo et al, 1998;Santos et al, 1996;Souto et al, 1996, Souto et al, 1998Alves et al, 2001;Pinheiro et al, 1996;Amorim et al, 1996;Pereira et al, 1996;Gusmão et al, 1997;Espinheira et al, 1996;Miranda et al, 1998;Prata et al, 1998) nity. This date on phenotypic ethnic classification is based on facial morphological characteristics and skin color in body regions not exposed to the sun (Azevêdo, 1980).…”

Genetic structure of north-west Africa revealed by STR analysis

“…Geographic location is shown in Figure 3. Allele frequencies for 13 loci in a partial subsample of Arabs and northern Berbers are given by Pérez-Lezaun et al 4 DNA was extracted from fresh whole blood using standard phenol-chloroform methods. Appropriate informed consent was obtained from all participants in this study and in most cases information about geographic origin and native language of their four grandparents was recorded.…”

“…Appropriate informed consent was obtained from all participants in this study and in most cases information about geographic origin and native language of their four grandparents was recorded. 4 Single locus amplifications were carried out in a final reaction volume of 10 µl according to the following touchdown PCR cycling conditions: 94°C for 1 min, 14 cycles at 94°C for 20 s, with annealing temperatures decreasing from 63°C by 0.5 degrees C in each cycle for 1 min, and 72°C for 1 min, 20 cycles at 94°C for 20 s, 56°C for 45 s, and 72°C for 1 min, plus a final extension cycle at 72°C for 5 min. Amplification products were run in an ABI 377™ sequencer, and ABI GS500 ROX and ABI GS500 TAMRA was used as internal lane standards.…”

“…4,5 There are original references for Portuguese (2n = 72) and Andalusians (2n = 68-72) 4 and Italians (2n = 446). 6 Population data for African Americans (2n = 390) and European Americans (2n = 400, also called Caucasians in the original reference) is available in AmpF/STR Profiler Plus and AmpF/ …”

The Genetic Makeup of Azoreans Versus Mainland Portugal Population

“…Consequently, the measure of diversity of neutral markers allows the inference of how these processes are shaping the overall signature of a population and has further implications in the general diseases apportionment. Genetic diversity values, based on autosomal STR markers, for Azores (0.788; Branco et al, 2008a;Santos et al, 2009) and mainland Portugal (0.782;Perez-Lezaun et al, 2000) indicate that both populations are very diverse. Studies of HLA markers in mainland (Spinola et al, 2005a; 3 loci) and in Azores (Spinola et al, 2005b; 6 loci) demonstrate values of average diversity of 0.92.…”