Allele Frequencies of Two Y-STRs in a Chinese Population

Abstract

A total of 74 blood samples were collected from unrelated males of Han ethnic group in Chongqing of China. DNA was extracted by utilizing Chelex method (1). Each PCR reaction contained 2-10 ng DNA, 1 X Taq buffer, 1.5 mM MgCl2, 200 RM each dNTP (Pharmacia Biotech, Sweden), 1.5 U Taq polymerase (NEB, UK), 0.3 RM each primer. PCR amplifications were performed in a GeneAmp PCR System 9600 (Perkin-Elmer, Foster City, CA),with denaturing for 2 min at 94°C, followed by 30 cycles of 94°C for 50 s, 58°C for 50 s and 72°C for 30 s. PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system, gels were silver stained (2). Alleles were designated according to recommendation of the International Society of Forensic Genetics (3). The gene diversities, the haplotypes diversity, and the standard errors of diversity were calculated in accordance with Hou's method (4).

Effect of Current Collector and Pyrolysis Temperature on the Electrochemical Performance of Photoresist Derived Carbon Films

“…The selection of SS wafer as a substrate is more apt as the same is used in the commercial Li-ion battery. 29 Later we have varied the pyrolysis temperature between 973 K and 1273 K with an interval of 100 K to investigate the effect of pyrolysis temperature on the crystalline structure and hydrogen content of the hard C films prepared from the SU-8 through C-MEMS process. Further, this change in the microstructure of thin C films and electronic conductivity of the current collector has been correlated to their electrochemical properties in terms of lithium ion intercalation.…”

In situ synthesis of [Cu(BODN)·5H2O]n@nano-Al composite energetic films with tunable properties in pyro-MEMS

“…4d), the absorption peaks at 1632 cm −1 , 1265 cm −1 and 734 cm −1 are attributed to the asymmetric stretching vibration, symmetrical stretching vibration and deformation vibration of –ONO 2 , respectively. 37 Meanwhile, the remaining absorption peaks located at 1060 cm −1 , 990 cm −1 , 811 cm −1 , and 670 cm −1 are attributed to the stretching vibration of C–O–C in cellulose backbones. In addition, as shown in Fig.…”