Alleles of<i>afd1</i>dissect REC8 functions during meiotic prophase I

Golubovskaya, Inna N.; Hamant, Olivier; Timofejeva, Ljudmilla; Wang, Rachel; Braun, David; Meeley, Robert B.; Cande, W. Zacheus

doi:10.1242/jcs.03054

Cited by 145 publications

(177 citation statements)

References 40 publications

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“…In budding yeast, the meiosis-specific cohesion component REC8 binds preferentially to AT-rich regions (axis association sites) during the development of axis structures to produce co-oriented linear arrays of loops (Zickler and Kleckner, 1999;Blat et al, 2002). In Arabidopsis and maize (Zea mays), homologs of REC8 are required for homologous pairing and recombination, largely by influencing the chromatin structure (Bhatt et al, 1999;Cai et al, 2003;Chelysheva et al, 2005;Golubovskaya et al, 2006). Rice REC8 was recently identified, and its depletion resulted in a lack of homologous pairing and failure to load key rice meiotic proteins, including PAIR2, PAIR3, MER3, and ZEP1, onto the chromosomes (Wang et al, 2009Shao et al, 2011).…”

Section: Role Of Rpa2c In Meiotic Recombinationmentioning

confidence: 99%

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Chang

Xin

et al. 2013

The Plant Cell

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Replication protein A (RPA) is a conserved heterotrimeric protein complex comprising RPA1, RPA2, and RPA3 subunits involved in multiple DNA metabolism pathways attributable to its single-stranded DNA binding property. Unlike other species possessing a single RPA2 gene, rice (Oryza sativa) possesses three RPA2 paralogs, but their functions remain unclear. In this study, we identified RPA2c, a rice gene preferentially expressed during meiosis. A T-DNA insertional mutant (rpa2c) exhibited reduced bivalent formation, leading to chromosome nondisjunction. In rpa2c, chiasma frequency is reduced by ;78% compared with the wild type and is accompanied by loss of the obligate chiasma. The residual ;22% chiasmata fit a Poisson distribution, suggesting loss of crossover control. RPA2c colocalized with the meiotic cohesion subunit REC8 and the axis-associated protein PAIR2. Localization of REC8 was necessary for loading of RPA2c to the chromosomes. In addition, RPA2c partially colocalized with MER3 during late leptotene, thus indicating that RPA2c is required for class I crossover formation at a late stage of homologous recombination. Furthermore, we identified RPA1c, an RPA1 subunit with nearly overlapping distribution to RPA2c, required for ;79% of chiasmata formation. Our results demonstrate that an RPA complex comprising RPA2c and RPA1c is required to promote meiotic crossovers in rice.

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Section: Role Of Rpa2c In Meiotic Recombinationmentioning

confidence: 99%

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Chang

Xin

et al. 2013

The Plant Cell

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“…For example, in maize, impairments in AFD1 result in defective telomere bouquets, indicating that the elongation of AEs on chromosomes is required for bouquet formation [10]. In rice, installation of PAIR2 depends on the normal formation of AE.…”

Section: Osam1 Functions In Early Meiotic Stagesmentioning

confidence: 99%

“…Cohesin proteins, which are thought to be involved in this process, have been extensively studied in yeast and animals [4][5][6]. In plants, some cohesin proteins have been characterized for their influence in meiotic pairing, especially the REC8 homologs: SYN1/DIF1 in Arabidopsis [7][8][9], AFD1 in maize [10] and OsRAD21-4 in rice [11]. It is becoming evident that the cohesin complex provides the basis for AE assembly and is implicated in various meiotic events [4,12].…”

Section: Introductionmentioning

confidence: 99%

OsAM1 is required for leptotene-zygotene transition in rice

et al. 2011

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The events occurring at the onset of meiosis have not been fully elucidated. In the present study, OsAM1 was identified in rice (Oryza sativa L.) by map-based cloning. OsAM1, a homolog of Arabidopsis SWI1 and maize AM1, encodes a protein with a coiled-coil domain in its central region. In the Osam1 mutant, pollen mother cells are arrested at leptotene, showing that OsAM1 is required for the leptotene-zygotene transition. Immunocytological analysis revealed that OsAM1 exists as foci in early prophase I meiocytes. Very faint OsREC8 foci persisted in the Osam1 mutant, indicating that OsAM1 is not required for the initial meiotic recruitment of OsREC8. In the absence of OsAM1, many other critical meiotic components, including PAIR2, ZEP1 and OsMER3, could not be correctly installed onto chromosomes. In contrast, in pair2, Osmer3 and zep1 mutants, OsAM1 could be loaded normally, suggesting that OsAM1 plays a fundamental role in building the proper chromosome structure at the beginning of meiosis.

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“…The mutation of Rec8 of yeast disrupts cohesion at both chromosome arms and centromeres, resulting in reduced recombination, alterations in synaptonemal complex formation, and premature separation of sister chromatids at meiosis I (Klein et al, 1999;Watanabe and Nurse, 1999;Nasmyth, 2001;Kitajima et al, 2003). In recent years, homologs of yeast REC8 have been identified in mice (MmRec8; Bannister et al, 2004), humans (HsRec8; Parisi et al, 1999), Caenorhabditis elegans (CeRec8; Pasierbek et al, 2001), Arabidopsis (Arabidopsis thaliana; SYN1; Bai et al, 1999), and maize (Zea mays; AFD1; Golubovskaya et al, 2006). Depletion of this protein leads to defects in meiosis.…”

mentioning

confidence: 99%

“…By contrast, SYN1 is necessary not only to maintain centromere cohesion at anaphase I but also for the monopolar orientation of kinetochores during the first meiotic division in Arabidopsis (Chelysheva et al, 2005). In maize, AFD1/REC8 controls homologous pairing through its role in axial element elongation and the subsequent distribution of the recombination machinery (Golubovskaya et al, 2006). Rice (Oryza sativa) is a model organism for monocots that represents another excellent system for studying the molecular mechanism of meiosis in high plants.…”

mentioning

confidence: 99%

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

Shao

Tang²,

Wang³

et al. 2011

Plant Physiol.

122

119

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The successful transmission of chromosomes during mitosis and meiosis relies on the establishment and subsequent release of cohesion between replicated chromatids. Cohesion is mediated by a four-subunit structural maintenance of chromosome complex, called cohesins. REC8 is a key component of this meiotic cohesion complex in most model organisms studied to date. Here, we isolated and dissected the functions of OsREC8, a rice (Oryza sativa) REC8 homolog, using two null Osrec8 mutants. We showed that OsREC8 encodes a protein that localized to meiotic chromosomes from approximately meiotic interphase to metaphase I. Homologous pairing and telomere bouquet formation were abnormal in Osrec8 meiocytes. Furthermore, fluorescent in situ hybridization experiments on Osrec8 meiocytes demonstrated that the mutation eliminated meiotic centromeric cohesion completely during prophase I and also led to the bipolar orientation of the kinetochores during the first meiotic division and accordingly resulted in premature separation of sister chromatid during meiosis I. Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8. By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins. These results suggest that OsREC8 has several essential roles in the meiotic processes.

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Alleles ofafd1dissect REC8 functions during meiotic prophase I

Cited by 145 publications

References 40 publications

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

OsAM1 is required for leptotene-zygotene transition in rice

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

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